Effects of Gelam and Acacia honey acute administration on some biochemical parameters of Sprague Dawley rats

Abstract

BackgroundSince ancient times, honey has been used for medicinal purposes in many cultures; it is one of the oldest and most enduring substances used in wound management. Scientific evidence for its efficacy is widely studied, but systemic safety studies are still lacking. It is essential to study the impact of consumption of honey on the health and proper development of the consumer. Therefore, the present study was designed to observe the effects of acute administration (14 days) of Gelam honey (GH), a wild harvesting honey and Acacia honey (AH), a beekeeping honey, on male and female Sprague Dawley (SD) rats.MethodsAn acute oral study was performed following OECD test guideline 423, with minor modifications. In the study, GH, AH and sucrose (S) were administered at 2000 mg/kg body weight. Animals were observed for the next 14 days. Gross pathology was performed at the end of the study. Animals were observed for mortality, morbidity, body weight changes, feed and water intake. Clinical biochemistry, gross pathology, relative organ weight and histopathological examination were performed.ResultsRats fed with honey did not exhibit any abnormal signs or deaths. Results showed a decrease in weight gain and energy efficiency, but significantly increased in total food intake and total calories in female rats fed with GH, compared to control (p < 0.05). Nevertheless, a significant increase in body weight was observed in male rats in all honey-treated groups. Male rats fed with AH significantly decreased in total food intake, total calories and energy efficiency. Both male and female rats fed with GH displayed a significant decrease in triglycerides compared to control group. Hepatic and renal function levels were within acceptable range. The gross necropsy analysis did not reveal changes in any of the organs examined.ConclusionsOur results suggest that acute consumption of GH and AH at 2000 mg/kg body weight of male and female SD rats has some discrepancy effects on biochemical parameters but in line with OECD regulation. Gelam honey may have potential in controlling weight gain and triglyceride levels in female rats compared to Acacia honey. SD rats have some effect on biochemical parameters, an exploration of which would make for intriguing analysis.

Bitter Gourd Honey Ameliorates Hepatic and Renal Diabetic Complications on Type 2 Diabetes Rat Models by Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Mechanisms

“…Intubation of 2 g/kg/day BGH significantly reduced the liver damage, which was almost similar to the effect of glibenclamide. Similar results were observed in rats treated with Tualang, Gleam, and Apica honey [50][51][52]. The increased urea and creatinine levels in the serum indicates impaired renal function [53].…”

<p>Superiority of L-tartaric Acid Modified Chiral Mesoporous Silica Nanoparticle as a Drug Carrier: Structure, Wettability, Degradation, Bio-Adhesion and Biocompatibility</p>

“…According to Table 3, almost no significant difference was detected in all biochemical parameters after administration of CMSN (except GLB and TP). 18,[42][43][44] The results indicated that CMSN did not promote severe abnormalities of the hepatic and renal functions, and was proven to be safe in vivo. Based on the in vitro and in vivo results, it was reasonable to speculate that CMSN was a biocompatible bio-material with negligible toxicity, and was suitable for further applications in biological fields.…”

Insights into Putative Health Implications of Gelam (Melaleuca cajuputi) Honey: Evidence from In-Vivo and In-Vitro Studies

“…In an animal model, Samat et al [ 73 ] observed the effects of GH acute administration on biochemical parameters. Rats fed with 2000 mg/kg body weight of GH did not show any adverse effects or deaths.…”