Effects of GDNF-Transfected Marrow Stromal Cells on Rats with Intracerebral Hemorrhage

Deng, Li; Gao, Xiaoqing; Fan, Guangbi; Yang, Chaoxian

doi:10.1016/j.jstrokecerebrovasdis.2019.06.002

Cited by 15 publications

(7 citation statements)

References 43 publications

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“…In addition, glial cell-derived neurotrophic factor (GDNF) was also recognized as an important neurotrophic factor in the growth, differentiation, development, maintenance, and injury repair for several types of neurons. Not only transfection but also drug-induced increase of GDNF could significantly enhance the functional improvement of grafted MSCs on ICH (Yang et al, 2011;Lee et al, 2015;Deng et al, 2019). Furthermore, delivered hypoxia-preconditioned MSCs could reduce the tissue loss of the ipsilateral striatum, and enhance neuroregeneration and neurological functional recovery after ICH, which was attributed to the neuronal nourish and protect effect of MSC-mediated upregulation of GDNF, VEGF, and BDNF (Sun et al, 2015).…”

Section: Msc Transplantation In Rodentsmentioning

confidence: 97%

“…In addition, the CX3CR1 overexpressed MSCs increased the viability and migration ability of transplanted MSCs, and improved the sensory and motor functions of the collagenase induced mice ICH model . Moreover, GDNF-transfected MSCs could improve the neurological function of experimental ICH rats through enhancing neurotrophic factor secretion (Deng et al, 2019). Therefore, miRNA-based MSC therapy was expected to purposefully regulate the proliferation and differentiation ability of MSCs in the pathological microenvironment of ICH.…”

Section: Preconditioning Treatmentsmentioning

confidence: 99%

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Mesenchymal Stem Cells Transplantation in Intracerebral Hemorrhage: Application and Challenges

Gong

Hao

Wang

2021

Front. Cell. Neurosci.

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Intracerebral hemorrhage (ICH) is one of the leading causes of death and long-term disability worldwide. Mesenchymal stem cell (MSC) therapies have demonstrated improved outcomes for treating ICH-induced neuronal defects, and the neural network reconstruction and neurological function recovery were enhanced in rodent ICH models through the mechanisms of neurogenesis, angiogenesis, anti-inflammation, and anti-apoptosis. However, many key issues associated with the survival, differentiation, and safety of grafted MSCs after ICH remain to be resolved, which hinder the clinical translation of MSC therapy. Herein, we reviewed an overview of the research status of MSC transplantation after ICH in different species including rodents, swine, monkey, and human, and the challenges for MSC-mediated ICH recovery from pathological microenvironment have been summarized. Furthermore, some efficient strategies for the outcome improvement of MSC transplantation were proposed.

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Section: Msc Transplantation In Rodentsmentioning

confidence: 97%

Section: Preconditioning Treatmentsmentioning

confidence: 99%

Mesenchymal Stem Cells Transplantation in Intracerebral Hemorrhage: Application and Challenges

Gong

Hao

Wang

2021

Front. Cell. Neurosci.

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“…This study found that treatment with GLC induced the expression of BDNF. Previous studies determined that BDNF stimulates neural progenitor cells to differentiate into mature neurons, and it exerts a neurotrophic effect at sites of injury (Shimotake et al, 2010;Deng et al, 2019). Previous studies have shown that neuroinflammation reduces the expression of BDNF and negatively affects many stages of neurogenesis (Hashimoto, 2015;Zhang et al, 2018;Zhong et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Glibenclamide Attenuates Neuroinflammation and Promotes Neurological Recovery After Intracerebral Hemorrhage in Aged Rats

Jiang

Zhang

Wang

et al. 2021

Front. Aging Neurosci.

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Intracerebral hemorrhage (ICH) is a common disease in the elderly population. Inflammation following ICH plays a detrimental role in secondary brain injury, which is associated with a poor prognosis of patients with ICH, and no efficient pharmacological preventions are available. Here, we investigated the effects of glibenclamide (GLC) on neuroinflammation in an autoblood-induced aged rat (18 months old) model of ICH. Rats were randomized into the sham, vehicle, and GLC groups. First, we investigated the expression level of sulfonylurea receptor 1 (Sur1) surrounding the hematoma after ICH. Then, neurological scores were calculated, and water maze tests, brain water content analysis, western blotting, and immunofluorescence assays were implemented to detect the neuroprotective effect of GLC. The expression of the Sur1-Trpm4 channel was significantly increased in the perihematomal tissue following ICH in aged rats. The GLC administration effectively reduced brain edema and improved neurofunction deficits following ICH. In addition, GLC increased the expression of brain-derived neurotrophic factors and decreased the expression of proinflammatory factors [tumor necrosis factor (TNF)-α,interleukin (IL)-1, and IL-6]. Moreover, GLC markedly reduced Ikappa-B (IκB) kinase (IKK) expression in microglia and nuclear factor (NF)-κB-P65 levels in perihematomal tissue. GLC ameliorated ICH-induced neuroinflammation and improved neurological outcomes in aged rats. In part, GLC may exert these effects by regulating the NF-κB signaling pathway through the Sur1-Trpm4 channel.

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“…Likewise, Yang et al reported that GDNF-transfected BMSC transplantation for ICH caused significantly lowered apoptosis and consequent better functional recovery [28]. Similarly, Deng et al have suggested that GDNF-transfected MSC have the potential to promote neurological recovery [29]. Thus, HGF and GDNF are also important trophic factors in stem cell therapy for ICH.…”

Section: Stem Cells Internationalmentioning

confidence: 99%

Intracerebral Transplantation of Mesenchymal Stromal Cell Compounded with Recombinant Peptide Scaffold against Chronic Intracerebral Hemorrhage Model

Takamiya

Kawabori

Kitahashi

et al. 2022

Stem Cells International

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Background. Due to the lack of effective therapies, stem cell transplantation is an anticipated treatment for chronic intracerebral hemorrhage (ICH), and higher cell survival and engraftment are considered to be the key for recovery. Mesenchymal stromal cells (MSCs) compounded with recombinant human collagen type I scaffolds (CellSaics) have a higher potential for cell survival and engraftment compared with solo-MSCs, and we investigated the validity of intracerebral transplantation of CellSaic in a chronic ICH model. Methods. Rat CellSaics (rCellSaics) were produced by rat bone marrow-derived MSC (rBMSCs). The secretion potential of neurotrophic factors and the cell proliferation rate were compared under oxygen-glucose deprivation (OGD) conditions. rCellSaics, rBMSCs, or saline were transplanted into the hollow cavity of a rat chronic ICH model. Functional and histological analyses were evaluated, and single-photon emission computed tomography for benzodiazepine receptors was performed to monitor sequential changes in neuronal integrity. Furthermore, human CellSaics (hCellSaics) were transplanted into a chronic ICH model in immunodeficient rats. Antibodies neutralizing brain-derived neurotrophic factor (BDNF) were used to elucidate its mode of action. Results. rCellSaics demonstrated a higher secretion potential of trophic factors and showed better cell proliferation in the OGD condition. Animals receiving rCellSaics displayed better neurological recovery, higher intracerebral BDNF, and better cell engraftment; they also showed a tendency for less brain atrophy and higher benzodiazepine receptor preservation. hCellSaics also promoted significant functional recovery, which was reversed by BDNF neutralization. Conclusion. Intracerebral transplantation of CellSaics enabled neurological recovery in a chronic ICH model and may be a good option for clinical application.

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Effects of GDNF-Transfected Marrow Stromal Cells on Rats with Intracerebral Hemorrhage

Cited by 15 publications

References 43 publications

Mesenchymal Stem Cells Transplantation in Intracerebral Hemorrhage: Application and Challenges

Mesenchymal Stem Cells Transplantation in Intracerebral Hemorrhage: Application and Challenges

Glibenclamide Attenuates Neuroinflammation and Promotes Neurological Recovery After Intracerebral Hemorrhage in Aged Rats

Intracerebral Transplantation of Mesenchymal Stromal Cell Compounded with Recombinant Peptide Scaffold against Chronic Intracerebral Hemorrhage Model

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