Effects of Fucoidan from Mozuku on Human Stomach Cell Lines

Abstract

Cladosiphon okamuranus (Okinawamozuku) is a species of Mozuku known to contain the most fucoidan of any brown algae. Fucoidan is a sulfated hetero polysaccharide with various biological activities. We isolated and purified fucoidan from C. okamuranus. The yield of fucoidan was +.*ῌ (w/w) based on wet algae from C. okamuranus. The sulfate content and molecular weight of the fucoidan were 3.2ῌ (w/w) and approximately -,,**,***, respectively. We examined the e#ects of fucoidan on human stomach cell lines. Fucoidan from C. okamuranus showed an inhibitory e#ect on anticancer agent /-fluorouracil (/-FU) in Hs 011.st normal stomach cells with minimum inhibition of the original e#ect of /-FU in cancer cells. Furthermore, fucoidan from C. okamuranus showed growth inhibition of stomach cancer cells but did not show any e#ects on normal cells. Thus, fucoidan from Mozuku, especially C. okamuranus, may be useful for cancer therapy.

Detection of Fucoidan in Urine after Oral Intake of Traditional Japanese Seaweed, <i>Okinawa mozuku</i> (<i>Cladosiphon okamuranus</i> Tokida)

“…Japanese people are familiar with most mozuku species and consume them as daily food (3). Mozuku, as well as konbu and wakame, contains approximately 1% fucoidan, based on wet weight (4).…”

“…Fucoidan is mainly composed of fucose and sulfate bases, each with a molecular weight typically of a magnitude of 1,000 kDa, and the molecular weight of fucoidan from Okinawa mozuku is 3,200 kDa (4,5). Fucoidan is known to have various biological activities including antithrombotic (6), anti-inflammatory (7-9), antiviral (10,11), anti-adhesive (12), and antitumor (13)(14)(15) activity.…”

Microbial Fucoidan Degradation byLuteolibacter algaeH18 with Deacetylation

“…It was extracted and purified from C. okamuranus by the method of Kawamoto et al 12) All other chemicals used were of the highest commercially available grade.…”

Isolation and Characterization of a Novel Fucoidan-Degrading Microorganism

“…As a high-molecular-weight fucoidan, we used ''SEA ALGA-F'' (Marine Products Kimuraya, Sakaiminato, Japan), which was extracted and purified from C. okamuranus by the method of Kawamoto et al 12) It contained 41.3% (w/w) fucose, 16.6% (w/w) uronic acid (calculated as glucuronic acid), 14.2% (w/w) sulfate, 17.8% (w/w) ash, and 8.5% (w/w) water. All of the other chemicals used were of the highest grade commercially available.…”