Isolation and Characterization of a Novel Fucoidan-Degrading Microorganism

Ohshiro, Takashi; Ohmoto, Yoshiyuki; Ono, Yoshihisa; Ohkita, Ryo; Miki, Yasuaki; Kawamoto, Hitoshi; Izumi, Yoshikazu

doi:10.1271/bbb.100327

Cited by 14 publications

(7 citation statements)

References 18 publications

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“…F31, 11) but separation of strain F31 cells from the culture broth by centrifugation was extremely problematical, because strain F31 appeared to produce viscous substances. Besides strain F31, we isolated bacteria showing an ability to grow in media containing fucoidan as sole carbon source.…”

Section: )mentioning

confidence: 99%

“…F31, degrading the fucoidan of C. okamuranus, and found that the strain had intracellular enzymes desulfating fucoidan, besides lowering its molecular weight. 11) In addition, no desulfation activity was detected until the molecular weight of fucoidan was decreased to less than several tens of thousand by enzymatic degradation.…”

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confidence: 99%

“…One strain, designated H18, was selected for further experiments. Whole DNA was extracted from cells of strain H18, as previously reported, 11) and the 16S rDNA was amplified by PCR using universal bacterial 16S rDNA primers 27f (5 0 -AGAGTTTGATCCTGGCTCAG-3 0 ) and 1525r (5 0 -AAAGGAGGTGATCCAGCC-3 0 ). The amplified DNA fragment, of approximately 1,500 bp, was then purified using a QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany) and then sequenced using universal 16S rDNA primers.…”

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confidence: 99%

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Microbial Fucoidan Degradation byLuteolibacter algaeH18 with Deacetylation

Ohshiro¹,

Harada²,

Kobayashi³

et al. 2012

Bioscience, Biotechnology, and Biochemistry

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Microbial Fucoidan Degradation byLuteolibacter algaeH18 with Deacetylation

Ohshiro¹,

Harada²,

Kobayashi³

et al. 2012

Bioscience, Biotechnology, and Biochemistry

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“…The preparation of obtained clones followed purification of recombinant DNA was carried out as described previously [Lin et al, 2009;Ohshiro et al, 2010;Rattanachomsri et al, 2011]. Escherichia coli DH5á and recombinant E. coli were cultured in LB containing 50 μg/mL ampicillin at 37 o C. Sequencing was performed with an ABI Prism BigDye Terminator Cycle Sequencing Ready Kit (Applied Biosystems, Foster City, CA) according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Influence of Chinese Milkvetch (Astragalus sinicus L.) with no-tillage on soil biotic factors and rice yield

Kim

Lee

Kang

et al. 2011

J Korean Soc Appl Biol Chem

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The bacterial community, biotic factors, and rice yield of Chinese Milkvetch (Astragalus sinicus L.) with no-tillage and conventional chemical fertilizer treatment were evaluated. Analysis of bacterial isolates from each treatment plots with 16S rDNA sequences revealed 486 isolates from 18 different bacterial species. The frequency of spore-forming bacteria, including Bacillus sp. and some Actinomycetes appeared to be high under low temperature conditions, whereas Proteobacteria disappeared at low temperature. Most extracellular hydrolytic enzyme activity showing bacteria belong to low G + C Gram(+) bacteria (LGCGPB) compared to high G + C Gram(-) bacteria (HGCGPB) and Proteobacteria. In addition, the total soil carbon content in Chinese Milkvetch treated soil was higher than that of conventional tilled soil samples. Rice yield was slightly higher in the conventionaltilled field (4.13±0.08 Mg/ha) compared to the Chinese Milkvetch-treated field (3.94±0.15 Mg/ha). However, the field treated with Chinese Milkvetch followed by no-tillage was effective in sustaining LGCGP bacterial species that promote biogeochemical cycling and enrich microbial biomass on paddy soil (3.94±0.15 Mg/ha). These results showed that the Chinese Milkvetch treatment with no-tillage is effective in conserving the soil environment without significant yield loss.

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“…Lfucanase is an example of endofucoidanase obtained from F. algae, which can produce fuco-oligosaccharides with immunomodulatory activity. Exofucoidanases act on the non-reducing end of sulfated fucose residues to produce oligomers and reduce the molecular weight at a relatively slower rate (Berteau and Mulloy, 2003;Ohshiro et al, 2010;Silchenko et al, 2013).…”

Section: In Vitro Enzymatic Degradation Of Marine Sulfated Polysacchamentioning

confidence: 99%

Absorption, distribution, metabolism and elimination (ADME) and toxicity profile of marine sulfated polysaccharides used in bionanotechnology

Majee¹,

Avlani²,

Ghosh³

et al. 2018

Afr. J. Pharm. Pharmacol.

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Sulfated polysaccharides extracted from marine algae and bacteria constitute an important class of biomacromolecules as they are characterized by biocompatibility, biodegradability and low immunogenicity. Recent advances in bionanotechnology are attributed to identification of marine sulfated polysaccharides of unique composition and functional properties. Promising results obtained so far justify the need for additional research in the study of absorption, distribution, metabolism and elimination (ADME) of these novel biopolymer-based nanomaterials in human body after administration by oral or parenteral route for therapeutic or diagnostic purpose. In vitro enzymatic degradation pathways should be investigated in order to yield commercially valuable oligomers. The goal of the present review is to enlighten on the ADME, cytotoxicity and in vitro enzymatic degradation of three marine sulfated polysaccharides, fucoidan, ulvan and mauran, obtained from brown seaweeds or macroalgae in the class of Phaeophyceae, members of Ulvales (green algae) and halophilic bacteria, respectively. They are presently being exploited in fabrication of nanoplatforms with novel applications in the field of controlled drug delivery, tissue regeneration scaffolds, cancer therapy, and bioimaging. However, significant research still needs to be carried out to characterize ADME of mauran and to improve production of the biopolymers on a large scale in order to find out clinically relevant solutions to establish these sulfated polysachharide-based nanotools as novel bionanotechnology strategies in future.

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Isolation and Characterization of a Novel Fucoidan-Degrading Microorganism

Cited by 14 publications

References 18 publications

Microbial Fucoidan Degradation byLuteolibacter algaeH18 with Deacetylation

Microbial Fucoidan Degradation byLuteolibacter algaeH18 with Deacetylation

Influence of Chinese Milkvetch (Astragalus sinicus L.) with no-tillage on soil biotic factors and rice yield

Absorption, distribution, metabolism and elimination (ADME) and toxicity profile of marine sulfated polysaccharides used in bionanotechnology

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