Effects of follicle stimulating hormone (FSH) on follicular development, oocyte retrieval, and in vitro fertilization (IVF) in ewes during breeding season and seasonal anestrus

Stenbak, T.K.; Redmer, Dale A.; Berginski, H.R.; Erickson, Aron; Navanukraw, Chainarong; Toutges, Michelle; Bilski, Jerzy J.; Kirsch, James D; Kraft, Kim C.; Reynolds, Lawrence P.; Grazul-Bilska, Anna T.

doi:10.1016/s0093-691x(01)00542-8

Cited by 48 publications

(25 citation statements)

References 34 publications

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“…Moor et al (1985) reported that exogenous FSH administration at high levels could disrupt the biochemical functions of oocytes causing them to undergo premature activation, resulting in the oocytes being already aged at the time of ovulation or collection. Previous studies in our laboratory have indicated that in vivo FSH administration for 3 days results in a decline in the cleavage rate as compared with a 2-day treatment in ewes (Stenbak et al 2001). Since, we observed a decrease in Cx43 expression after a 3-day treatment with FSH, we can suggest that disruption of gap junction function by prolonged FSH treatment is affecting oocyte quality; however, this subject requires further study.…”

Section: Discussionsupporting

confidence: 44%

“…For day 15 of the estrous cycle, half the COCs were immediately embedded in agarose gel and then fixed in Carnoy's solution. The other half were incubated in maturation medium (TCM-199, 10% FBS, 5 mg/ml ovine FSH (oFSH-RP-1; NIAMDD-NIH, Bethesda, MD, USA), 5 mg/ml ovine LH (oLH-26; NIADDK-NIH), 1 mg/ml estradiol (0.5 ml/ml; Sigma), 2 mM glutamine (Sigma), 0.25 mM sodium pyruvate (Sigma), and penicillin -streptomycin (doses as above) Stenbak et al 2001) for 21 -24 h at 39 8C, 5% CO 2 and 95% air, at 100% humidity under mineral oil stabilized for 24 h at conditions listed above, and then embedded in agarose gel and fixed in Carnoy's solution. For day 16 of the estrous cycle, COCs were embedded in agarose gel and fixed immediately after collection.…”

Section: Animal Treatment and Coc Collection In Experimentsmentioning

confidence: 99%

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Expression of connexin 43 and gap junctional intercellular communication in the cumulus–oocyte complex in sheep

Pant¹,

Reynolds²,

Luther³

et al. 2005

Reproduction

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To evaluate the effects of FSH, LH and/or cAMP on expression of connexin 43 (Cx43) in the ovine cumulus-oocyte complex (COC) and gap junctional intercellular communication (GJIC) of cumulus cells, two experiments were carried out. In experiment 1, Cx43 was immunodetected in the COC, before or after maturation, obtained from non-treated or FSH-treated ewes. The expression of Cx43 in the COC was greater (P < 0.01) on day 16 than on day 15 of the estrous cycle. In vivo FSH treatment decreased (P < 0.02) Cx43 expression on day 16 but not on day 15 of the estrous cycle. In experiment 2, intact COCs or isolated cumulus cells obtained from small and large follicles from FSH-treated ewes were cultured with or without FSH, LH or cAMP agonist and evaluated for GJIC by laser cytometry. For large follicles, the basal rate of GJIC was greater (P < 0.01) for cumulus cells in intact COCs than for isolated cumulus cells. FSH increased (P < 0.04) GJIC in cumulus cells in intact COCs and tended to increase (P < 0.1) GJIC in isolated cumulus cells from small follicles but decreased (P < 0.01) GJIC in cumulus cells in intact COCs from large follicles. LH also increased (P < 0.01) GJIC in isolated cumulus cells from small follicles but decreased GJIC in intact COCs (P < 0.01) and isolated cumulus cells (P < 0.02) from large follicles. cAMP increased (P < 0.01) the GJIC in both intact COCs and cumulus cells from small and large follicles. These results indicate that day of estrous cycle, stage of maturation and duration of FSH treatment affect expression of Cx43 in ovine COCs. In intact COCs, GJIC in cumulus cells was enhanced, probably due to the presence of the oocyte. In addition, the effects of FSH and LH, but not cAMP, on GJIC of cumulus cells depended on the stage of follicular development and on the presence of the oocyte.

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Section: Discussionsupporting

confidence: 44%

Section: Animal Treatment and Coc Collection In Experimentsmentioning

confidence: 99%

Expression of connexin 43 and gap junctional intercellular communication in the cumulus–oocyte complex in sheep

Pant¹,

Reynolds²,

Luther³

et al. 2005

Reproduction

View full text Add to dashboard Cite

show abstract

“…However, the treatment with melatonin in anoestrus did not improve fertilization rate in superovulated ewes when compared with controls (McEvoy et al, 1998;Forcada et al, 2006). Likewise, Stenbak et al (2001) reported a higher in vitro fertilization (IVF) of oocytes recovered from superovulated ewes in the breeding season than during the anoestrus, whereas no differences in fertilization rate were elucidated after IVF of oocytes recovered from superovulated ewes treated or not with melatonin in anoestrus (Luther et al, 2005).…”

Section: Fertilization Ratementioning

confidence: 91%

Effect of exogenous melatonin on the ovary, the embryo and the establishment of pregnancy in sheep

Abecia¹,

Forcada²,

Casao³

et al. 2008

Animal

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Administration of melatonin to advance the breeding season in sheep has been widely used, since this hormone conveys the photoperiodic signal to the reproductive neuroendocrine axis. An increased lambing percentage has been reported following such treatment during anoestrus, which could be mediated through a higher rate of embryonic survival, either by an improvement in luteal function or by a reduction in the antiluteolytic mechanisms. The aim of this article is to review the body of knowledge on the effect of melatonin on the ovine ovary, the embryo and the establishment of pregnancy. Some studies using synchronized ewes have found that melatonin treatments during anoestrus do not improve ovulation rate by modifying the timing of follicle emergence, but increasing the number of ovulatory follicles by decreasing the atresia of medium and large follicles. On the other hand, the addition of melatonin to the in vitro maturation medium does not improve oocyte maturation rate in oocytes from sheep ovaries recovered either in anoestrus or in the breeding season. However, a luteotrophic effect of melatonin at either short or medium term has been reported. We have recently observed that melatonin implants tend to improve the survival of embryos collected from ewes after superovulation in anoestrus. More specifically, melatonin induced a significant reduction of the number and rate of non-viable (degenerate and retarded) embryos. Preliminary data from our laboratory suggest that the uterine sensitivity to progesterone -in terms of progesterone receptor expression -of superovulated ewes could be reduced by melatonin treatment. It can be concluded that the success of exogenous melatonin as a means to improve lamb production of sheep is due, at least in part, to an improvement of luteal support and embryonic survival.

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“…In sheep, some studies have suggested that seasonal anoestrous can have a significant detrimental effect on the number of recovered oocytes per female, the competence of the oocytes, and the in vitro fertilization rate (Stenbak et al, 2001;Vázquez et al, 2009); however, information about the effects of melatonin treatments on oocyte quality and in vitro fertilization (IVF) during anoestrous is limited. In one study, after IVF of the oocytes recovered from superovulated ewes in the nonreproductive season, melatonin-treated and untreated ewes did not differ significantly in their fertilization rates (Luther et al, 2005).…”

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confidence: 99%

The effects of melatonin on in vitro oocyte competence and embryo development in sheep

Casao

Abecia

Pérez

et al. 2010

Span. j. agric. res.

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The aim of this study was to assess the effects of melatonin on the in vitro maturation and fertilization of sheep oocytes, and on the in vitro culture of the embryos. Oocytes from sheep ovaries collected at the slaughterhouse were divided into four groups, two of which were treated with either 10E5 M (M5) or 10E6 M (M6) melatonin, while the other two groups served as untreated controls (C5 and C6). After in vitro fertilization with fresh ram semen, the embryos produced in each group were divided into two sets, one cultured with melatonin (M5M, C5M, M6M and C6M), and the other without melatonin (M5C, C5C, M6C, and C6C). A melatonin concentration of 10E6 M increased maturation rate (82.5% vs. 73.7% for M6 and C6, respectively; P < 0.05) and tended to improve cleavage rate 36 hours after in vitro fertilization (79.4% vs. 72.6% for M6 and C6, respectively, P = 0.08). A higher melatonin concentration (10E5 M) did not have significant effects on those parameters. Blastocyst rates on day 8 did not differ significantly among groups.

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Effects of follicle stimulating hormone (FSH) on follicular development, oocyte retrieval, and in vitro fertilization (IVF) in ewes during breeding season and seasonal anestrus

Cited by 48 publications

References 34 publications

Expression of connexin 43 and gap junctional intercellular communication in the cumulus–oocyte complex in sheep

Expression of connexin 43 and gap junctional intercellular communication in the cumulus–oocyte complex in sheep

Effect of exogenous melatonin on the ovary, the embryo and the establishment of pregnancy in sheep

The effects of melatonin on in vitro oocyte competence and embryo development in sheep

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