Effects of Flutriafol Fungicide on the Lipid Accumulation in Human Liver Cells and Rat Liver

Kwon, Hyuk-Cheol; Sohn, Hyejin; Kim, Dohyun; Jeong, Chang-Hee; Kim, Dong-Wook; Han, Sung Gu

doi:10.3390/foods10061346

Cited by 7 publications

(4 citation statements)

References 56 publications

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“…These results suggest that the increased liver weights and macrovesicular fatty change of the hepatocytes in the HFrD group were caused by the accumulation of triglycerides in the liver. In other words, the daily consumption of excessive fructose for 4 weeks induced a typical NAFLD-like situation, as seen in other reports (12,33,34). In this study, periportal vacuolation in the hepatocytes was observed in the HFrD group after 4-weeks consumption (Table II).…”

Section: Discussionsupporting

confidence: 87%

Novel Biomarker Establishment for Evaluation of Excessive Fructose Consumption Using a Rat Model

Suzuki

Kondo

Toyoda

et al. 2023

In Vivo

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Background/Aim: The habitual consumption of excessive fructose is associated with the onset and progression of lifestyle-related diseases, such as nonalcoholic fatty liver disease (NAFLD). In this study, we investigated the physiological changes observed when consuming a diet containing excessive fructose on the viewpoints of hepatotoxicity biological markers using a rat model and explored the biomarker candidates that could detect the effects of excessive fructose intake at an early stage. Materials and Methods: Male rats were fed 63% high fructose diet (HFrD) ad libitum and their blood samples were collected before and at 1, 2, 3, and 4 weeks after allocation. The plasma biochemical parameters, hepatotoxic enzyme activities including alkaline phosphatase (ALP) isozymes were analyzed. Results: HFrD consumption for 4-weeks created NAFLD-like symptoms, including elevated plasma lipid parameters and hepatotoxicity markers, as well as fat accumulation in the liver compared with rats consuming a control diet. Alanine aminotransferase (ALT) and glutamate dehydrogenase (GLDH) were increased from the 3 rd and 2 nd weeks, respectively, but no changes were observed on ALP activity. However, the daily consumption of the HFrD increased the plasma activities of liver-type ALP isozyme, and decreased plasma small intestinaltype ALP isozyme soon after the start of feeding. Conclusion: ALP isozyme analysis in combination with GLDH and ALT activities in the plasma samples could be a useful tool to detect the physiological changes induced by excessive fructose intake at an early stage of the development of NAFLD.

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Section: Discussionsupporting

confidence: 87%

Novel Biomarker Establishment for Evaluation of Excessive Fructose Consumption Using a Rat Model

Suzuki

Kondo

Toyoda

et al. 2023

In Vivo

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“…Body weight of flutriafol treated rats was increased compared to control at 1 and 2 wk. However, the body weights at 3 wk were not significantly different ( Kwon et al, 2021a ). No significant effects of tebuconazole treatment were observed on body condition, growth, and sex ratio of chicks ( Lopez-Antia et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

“…Exposure to ethylated dialkylphosphates, which are known to have immunomodulatory potential, can induce long-term damage to the heart, leading to fibrosis ( Medina-Buelvas et al, 2019 ). Exposure to residual pesticides increased liver fibrosis and nonalcoholic fatty liver disease in HepG2 cells and rat liver ( Kwon et al, 2021a ; Kwon et al, 2021b ). Furthermore, meta-analysis revealed that the risk of idiopathic pulmonary fibrosis increased in agricultural workers exposed to pesticides ( Park et al, 2021 ; Ramani et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of Flutriafol Exposure on Residue Characteristics in Pig Muscle and Fat Tissue

Jeong¹,

Kim²,

Ji³

et al. 2022

Food Sci Anim Resour

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This study investigated the effect of exposure to flutriafol based on residues in pigs. Pigs were exposed to different concentrations (0.313, 0.625, 3.125, 6.25, and 12.5 mg/kg bw/d, n=20) for 4 wk in different treatment groups. Serum biochemical analysis, residue levels, and histological analysis were conducted using the VetTest chemistry analyzer, liquid chromatography mass spectrometry, and Masson’s trichrome staining, respectively. The body weight (initial and final) was not significantly different between groups. Parameters such as creatinine, blood urea nitrogen, alanine aminotransferase, and lipase levels were significantly different as compared to the control group. Flutriafol increased the residue limits in individual tissue of the pigs in a dose dependent manner. Flutriafol exposures indicated the presence of fibrosis, as confirmed from Masson’s trichrome staining. These results suggest that flutriafol affects the morphology and serum levels in pigs. The dietary flutriafol levels can provide a basis for maximum residue limits and food safety for pork and related products.

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“…A previous study showed that insecticide exposure impaired lipid metabolism, thereby resulting in the development of NAFLD [21]. Moreover, recent studies have reported that fungicides such as propiconazole, flutriafol, cyproconazole, dazomet, fluazinam, hexaconazole, pyrasulfotole metabolite, and myclobutanil can cause NAFLD pathogenesis [22][23][24][25]. However, the toxic effects of TEB on lipid accumulation and lipid metabolism in the liver have rarely been studied.…”

Section: Discussionmentioning

confidence: 99%

Tebuconazole Fungicide Induces Lipid Accumulation and Oxidative Stress in HepG2 Cells

Kwon

Kim

Jeong³

et al. 2021

Foods

Self Cite

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Tebuconazole (TEB), a triazole fungicide, is frequently applied to agriculture for the increase of food production. Although TEB causes liver toxicity, its effects on cellular lipid accumulation are rarely investigated. Therefore, this study aimed to study the effects of TEB on lipid metabolism and accumulation in HepG2 cells. HepG2 cells were exposed to 0–320 µM TEB for 1–24 h. TEB (20–80 µM, 24 h)-treated cells showed lipid accumulation. Further, TEB (20–80 µM, 1–12 h) increased the nuclear translocation of peroxisome proliferator-activated receptors and the expression of lipid uptake and oxidation-related markers such as cluster of differentiation 36, fatty acid transport protein (FATP) 2, FATP5, and carnitine palmitoyltransferase 1. Oxidative stress levels in TEB-treated cells (20–80 µM, 24 h) were higher, compared to those in the control. TEB (20–80 µM, 24 h) also induced the loss of mitochondrial membrane potential and lower levels of microsomal triglyceride transfer protein in the cells. Thus, TEB can induce lipid accumulation by altering the expression of lipid-metabolizing molecules and can therefore impair lipid metabolism. Our data suggest that human exposure to TEB may be a risk factor for non-alcoholic fatty liver disease.

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Effects of Flutriafol Fungicide on the Lipid Accumulation in Human Liver Cells and Rat Liver

Cited by 7 publications

References 56 publications

Novel Biomarker Establishment for Evaluation of Excessive Fructose Consumption Using a Rat Model

Novel Biomarker Establishment for Evaluation of Excessive Fructose Consumption Using a Rat Model

Effect of Flutriafol Exposure on Residue Characteristics in Pig Muscle and Fat Tissue

Tebuconazole Fungicide Induces Lipid Accumulation and Oxidative Stress in HepG2 Cells

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