Effects of Fibroin Treatments on Physical and Biological Properties of Chitosan/Hydroxyapatite/Fibroin Bone's Scaffold

Abstract

The development of bio-mimetic scaffold for tissue engineering proposed a novel method to tissue or bone repairing. The biological and physical properties of the scaffold have been recognizing such as biocompatibility, porosity, pore size, and biodegradability. In this work, Chitosan, Hydroxyapatite (HA), and Fibroin were used for bone's scaffold fabrication by freeze drying technique. Those materials are known as biodegradable materials that serve different properties in bone's scaffold. In common fabrication process, the fibroin treatment is requiring for increasing the stiffness of the fibers. Recently, the fibroin treatment is process before the scaffold fabrication. However, the treatment could process after the scaffold fabrication complete. Thus, we compared the biological and physical of the scaffolds between three conditions of fibroin treatment that consist of 1) Non-treatment (NON), 2) Pre-treatment (PRE), and 3) Post-treatment (POST). From the result, both of biological and physical properties, the PRE porous scaffold is the appropriated condition for this research. Finally, we are looking forward to compare the growth of osteoblast cells on the scaffold with different fibroin treatment and aim to implant those scaffolds for bone repairing in the very near future.

The Possibility of Interlocking Nail Fabrication from FFF 3D Printing PLA/PCL/HA Composites Coated by Local Silk Fibroin for Canine Bone Fracture Treatment

“…Each cocoon was cut small and degummed in 100 • C Na 2 CO 3 solution for 30 min and rinsed with warm deionized (DI) water. The degummed silk fibers were dried overnight in a 37 • C hot air oven and then dissolved at 70 • C for 6 h in a ternary solvent (CaC l2 /CH 3 CH 2 OH/H 2 O; 1:2:8 in mole ratio) [31,32]. The silk-ternary solution was dialyzed using a cellulose membrane with 4 • C DI water for 3 days, and the DI water was changed every day.…”

“…mole ratio) [31,32]. The silk-ternary solution was dialyzed using a cellulose membrane with 4 °C DI water for 3 days, and the DI water was changed every day.…”

The 3D-Printed Bilayer’s Bioactive-Biomaterials Scaffold for Full-Thickness Articular Cartilage Defects Treatment

“…CS flakes derived from a squid pen with 94.69% deacetylation degree (Taming Enterprise co., Ltd., Samutsakon, Thailand) were cut into small pieces and soaked in 1% (v/v) acetic acid solution (100% acetic acid, Merck, Darmstadt, Germany) in the ratio of 0.5 g/100 mL, until completely dissolved (approximately 3–5 days). Then, 0.5 g of SF sponge [ 15 , 57 , 58 , 84 ] was added to the CS solution and homogeneously mixed. The 25% glutaraldehyde (GA: 25% in water, Merck, Darmstadt, Germany) was diluted in DI water (1/100, v/v) and used as a cross-linking agent.…”

“…HA is used in bone tissue engineering applications owing to its bioactivity, osteo-conductivity, and biodegradability [ 55 , 56 ]. HA powder can be added to other biomaterials to increase their biological properties [ 57 , 58 ]. Moreover, chitosan (CS) and silk fibroin (SF) were selected to be additional materials for AC layer fabrication.…”

“…CS is a type of polysaccharide derived from chitin with a structure similar to the glycosaminoglycans (GAGs), which are a component of the cartilage extracellular matrix [ 12 , 59 ]. CS is widely used in tissue engineering applications owing to its porous structure, biocompatibility, biodegradability, non-cytotoxicity, and low cost [ 57 , 58 , 60 ]. Furthermore, implantation of the CS scaffold could reduce the risk of bacterial infection owing to its antimicrobial property [ 61 , 62 , 63 ].…”