Effects of fetuin on zona pellucida hardening, fertilization and embryo development in cattle

Landim-Alvarenga, F. C.; Boyazoglu, S.; Carvalho, Lídia Raquel de; Choi, Young Ho; Squires, E.L.; Seidel, G. E.

doi:10.1016/s0378-4320(02)00045-3

Cited by 11 publications

(9 citation statements)

References 36 publications

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“…In our study, even the short exposure (10–30 min) to FCS during handling gave a developmental advantage (other than a zona‐protective effect) to oocytes, which was apparent on the ninth day of development in blastocyst rates. This is in accordance with the results of the study by Landim‐Alvarenga et al. (2002).…”

Section: Discussionsupporting

confidence: 93%

“…However, it was recently reported that cryopreservation does result in zona pellucida changes in bovine oocytes, which can be bypassed by sperm injection (Mavrides and Morroll 2005). According to Landim-Alvarenga et al (2002), fetuin at three different concentrations had no beneficial effect on cumulusintact bovine oocytes, but as little as 1 mg fetuin/ml prevented zona hardening of oocytes stripped at 8 h of maturation. We therefore chose this concentration to investigate the effects of fetuin on bovine oocyte vitrification and later development.…”

Section: Discussionmentioning

confidence: 99%

“…FCS also prevents zona hardening during spontaneous maturation in mouse and cattle oocytes (Eppig et al. 1992; Landim‐Alvarenga et al. 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Foetal calf serum (FCS), which is included in vitrification and handling media in many vitrification protocols (Martino et al 1996a;Vajta et al 1998), prevents zona hardening (George et al 1992;Carroll et al 1993), but not cortical granule exocytosis (Ducibella et al 1990;Vincent et al 1991). FCS also prevents zona hardening during spontaneous maturation in mouse and cattle oocytes (Eppig et al 1992;Landim-Alvarenga et al 2002). Fetuin, a component of FCS, inhibits zona hardening, possibly by preventing proteolytic conversion of zona protein 2 to zona protein 2f (Schroeder et al 1990).…”

Section: Introductionmentioning

confidence: 99%

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Use of Fetuin Before and During Vitrification of Bovine Oocytes

Horváth

Seidel

2007

Reprod Domestic Animals

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After vitrification of oocytes, fertilization rates and subsequent development are unsatisfactory, possibly due in part to zona hardening. Foetal calf serum (FCS) can prevent zona hardening because of its fetuin content, but FCS composition varies among batches, and may contain viruses. In this study, we therefore compared media supplemented with different sources of macromolecules, 2% bovine serum albumin (BSA), 2% BSA + 1 mg/ml fetuin and 20% FCS, for handling oocytes for 10-30 min prior to vitrification. None of the treatments resulted in developmental rates comparable with the non-vitrified controls, but FCS inclusion in pre-vitrification handling medium resulted in higher blastocyst production per oocyte (p < 0.05) (10.8%) on day 9 of culture than BSA (5.3%) or BSA + fetuin (6.4%). Blastocysts developing from oocytes from all vitrification treatments were somewhat retarded relative to those developed from non-vitrified oocytes. We also tested the use of fetuin during vitrification as well as two different exposure times with cryoprotectants, 180 and 30 s. There was no significant effect of fetuin or exposure time on rates of subsequent blastocyst production.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

“…FCS also prevents zona hardening during spontaneous maturation in mouse and cattle oocytes (Eppig et al. 1992; Landim‐Alvarenga et al. 2002).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Use of Fetuin Before and During Vitrification of Bovine Oocytes

Horváth

Seidel

2007

Reprod Domestic Animals

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“…We find that like naturally produced embryos, in vitro fertilized Scc1 Δ zygotes activate a checkpoint that delays entry into mitosis (Figure 7C). Bovine, and sometimes mouse, in vitro fertilizations are carried out in the presence of fetuin to increase the fertilization efficiency (Dietzel et al., 2013, Landim-Alvarenga et al., 2002). Unexpectedly, we found that fetuin leads to faster kinetics of mitotic entry (Figure 7C), suggesting that the checkpoint was attenuated under these conditions.…”

Section: Resultsmentioning

confidence: 99%

A Surveillance Mechanism Ensures Repair of DNA Lesions during Zygotic Reprogramming

Ladstätter

Tachibana-Konwalski

2016

Cell

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SummarySexual reproduction culminates in a totipotent zygote with the potential to produce a whole organism. Sperm chromatin reorganization and epigenetic reprogramming that alter DNA and histone modifications generate a totipotent embryo. Active DNA demethylation of the paternal genome has been proposed to involve base excision and DNA repair-based mechanisms. The nature and consequence of DNA lesions generated during reprogramming are not known. Using mouse genetics and chemical biology, we discovered that Tet3-dependent zygotic reprogramming generates paternal DNA lesions that are monitored by a surveillance mechanism. In vivo structure-function rescue assays revealed that cohesin-dependent repair of paternal DNA lesions prevents activation of a Chk1-dependent checkpoint that delays mitotic entry. Culturing conditions affect checkpoint stringency, which has implications for human in vitro fertilization. We propose the zygotic checkpoint senses DNA lesions generated during paternal DNA demethylation and ensures reprogrammed loci are repaired before mitosis to prevent chromosome fragmentation, embryo loss, and infertility.

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