Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalus bubalis) oocytes in vitro

Puri, G. S.; Chaudhary, Sudhir; Singh, Virendra; Sharma, Arun

doi:10.14202/vetworld.2015.143-146

Cited by 11 publications

(7 citation statements)

References 22 publications

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“…Thus, based on cumulus cells expansion, the in vitro maturation performance of bovine oocytes increased significantly (P< 0.05) in serum basic maturation media than serum free media which might be due to addition of bovine serum. The results recorded in the present experiment were in close agreement with the earlier reports [6,14,15] where they reported better maturation rates of bovine follicular oocytes than serum free medium. On the contrary, ovarian types (type I without CL and type II with CL) did not have any significant effect on in vitro maturation rate based on cumulus cells expansion and nuclear maturation.…”

Section: Resultssupporting

confidence: 93%

Effect of fetal bovine serum on in vitro maturation and fertilization of bovine oocytes

Baishya¹,

Bora²,

Goswami³

et al. 2020

J. Entomol. Zool. Stud.

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The present experiment was conducted to investigate the effect of fetal bovine serum on in vitro maturation and fertilization of bovine oocytes. The mean percentages of in vitro maturation performance of bovine oocytes based on cumulus cells expansion in both serum and serum free basic maturation media in type-I & type-II ovaries were recorded as 76.52 ± 3.95 and 64.81+ 4.60 & 73.33 ± 5.71 and 62.96 ± 6.57 respectively. The mean percentages of in vitro maturation based on cumulus cells expansion were recorded as 75.43 ± 3.25 and 64.20 ± 3.77 respectively in serum and serum free culture media irrespective of ovarian types. So, the mean percentage of in vitro maturation performance of bovine oocytes based on cumulus cells expansion increased significantly (P< 0.05) in serum basic maturation media than in serum free media in both type I and type II ovaries and also irrespective of ovarian types. The mean fertilization rates (%) were found to significantly higher (P< 0.05) in serum culture media (70.33 ± 3.21) than the serum free media (55.81 ± 4.33). The mean cleavage percentages recorded from 4-cell to 16-cell stages of embryos in serum culture media were found to be significantly higher (P< 0.05) than the serum free media.

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Section: Resultssupporting

confidence: 93%

Effect of fetal bovine serum on in vitro maturation and fertilization of bovine oocytes

Baishya¹,

Bora²,

Goswami³

et al. 2020

J. Entomol. Zool. Stud.

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“…Adding FCS to maturation media enhance oocytes maturation compare with BSA in buffalo (Deneke et al 2013). Similar results were reported in cattle (Sreenivas et al 2014;del Collado et al 2015), This may be due to the abundance of nutrients, growth factors, antioxidants in FCS ( Sreenivas et al 2014;Puri et al 2015). Also, its high content of lipid compared to BSA (del Collado et al 2015).…”

Section: Resultssupporting

confidence: 78%

Effect of Culture Media, Gonadotropins, Proteins, Growth Factors and Hyaluronic Acid on In-Vitro Maturation of Buffalo Oocytes

Khadr

abdou

El-Sherbiny

et al. 2022

Journal of Animal and Poultry Production

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This study was carried out to investigate the effect of culture media, gonadotropins, proteins, growth factors and hyaluronic acid (HA) on the IVM rate of buffalo oocytes. Cumulus oocyte complexes were aspirated from ovaries and classified into 4 Grades, excellent and good quality COCs. Oocyte maturation was tested using TCM199, CR1aa or DMEM (Experiment 1); 10 µg/mL FSH + 50 IU/mL eCG, 10µg/mL FSH or 50 IU/mL eCG (Experiment 2): 10% FCS, 4mg/ml BSA or 1 µg/mL PVA (Experiment 3). Control group;10 µg/mL IGF-1 or 10 µg/mL EGF (Experiment 4) or 0, 2.5, 5 and 10 µg HA (Experiment 5). In all experiments, COCs were cultured for 252-24 h, then cytoplasmic and nuclear maturation was conducted. Results indicated that TCM199 achieved the best cytoplasmic and nuclear maturation. TCM199 supplemented with FSH and/or eCG enhanced in-vitro cytoplasmic and nuclear maturation of buffalo oocytes. Adding FCS, using growth factors, or 2.5 µg/mL HA toTCM199 lead to improving maturation rate. the results indicated that in-vitro maturation of buffalo oocytes is improved in TCM-199 supplemented with 50 IU eCG, 10μg/mL FSH, 10% FCS, 10 μg/ml EGF or 2.5 μg/mL HA.

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“…Sreenivas et al (2013) achieved better maturation and cleavage when FBS supplemented in IVM and IVF media. Also Puri et al (2015) obtained higher maturation rate for buffalo oocytes in the presence of FBS as compared to Estrus Buffalo Serum (EBS). Similar results were obtained in the present study conducted using crossbred cattle oocytes.…”

Section: Discussionmentioning

confidence: 95%

“…Gordon (2003) reported that ECS had a significant and marked effect compared with FCS on the developmental competence of bovine oocytes. In contrary, Puri et al (2015) obtained higher maturation rate for buffalo oocytes in the presence of fetal bovine serum (FBS) as compared to estrus buffalo serum (EBS). The objective of the present study was to examine the effect of FCS and mid ECS in culture media on IVM and IVF of oocytes from crossbred cattle under tropical conditions.…”

Section: Introductionmentioning

confidence: 96%

Effect of Fetal Calf Serum and Mid Estrus Cow Serum on In Vitro Maturation and Fertilization of Oocytes from Crossbred Cattle in the Tropics

Vettical

2016

Int J Appl Sci Biotechnol

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The objective of the study was to examine the effect of fetal calf serum (FCS) and mid estrus cow serum (ECS) in culture media on in vitro maturation (IVM) and in vitro fertilization (IVF) of oocytes from crossbred cattle in the tropics. Oocytes from abattoir ovaries were cultured in three different maturation media at 39oC temperature, 5% CO2 tension with maximum humidity for 24 h. TCM-199 containing 25mM HEPES, 1mM glutamine L, 2.2mg/mL sodium bicarbonate, antibiotics, 22 µg/mL pyruvate, 1µg/mL estradiol-17β, 0.5µg/mL FSH and 0.06 IU hCG without serum supplement used as treatment-1 and the above media were further supplemented with 10% FCS as treatment-2. In treatment-3, instead of 10% FCS, 20% heat inactivated ECS (serum collected in mid estrum) was used as serum supplement. Oocytes with maximum degree of cumulus expansion were selected as matured and used for further IVF studies using frozen semen. The IVF medium consisted of Fert-TALP medium supplemented with 1µM epinephrine, 10µM hypotaurine, 20µM pencillamine and 0.56µg/ml heparin. Culture conditions set for IVF were 39oC temperature, 5% CO2 tension with maximum humidity. Oocytes showing sperm penetration evidence like presence of enlarged sperm head, male pronuclei with its accompanying sperm tail in the cytoplasm, oocytes with two pronuclei and a clear second polar body but without a sperm tail were considered as fertilized. Significantly higher result of cumulus expansion percentage (p< 0.05) was observed when oocytes matured in media supplemented with FCS as compared to other two treatments. There was no influence of source of serum in maturation media on further IVF of matured oocytes in this study. Int J Appl Sci Biotechnol, Vol 4(3): 308-310

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Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalus bubalis) oocytes in vitro

Cited by 11 publications

References 22 publications

Effect of fetal bovine serum on in vitro maturation and fertilization of bovine oocytes

Effect of fetal bovine serum on in vitro maturation and fertilization of bovine oocytes

Effect of Culture Media, Gonadotropins, Proteins, Growth Factors and Hyaluronic Acid on In-Vitro Maturation of Buffalo Oocytes

Effect of Fetal Calf Serum and Mid Estrus Cow Serum on In Vitro Maturation and Fertilization of Oocytes from Crossbred Cattle in the Tropics

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