Effects of fertilizer practice on fungal and actinobacterial cellulolytic community with different humified particle-size fractions in double-cropping field

Abstract

Cellulose plays an important role in maintaining or improving soil carbon (C) cycling and soil fertility of paddy field. There had close relationship between functional cellulose genes (cbhI and GH48) with characterize of soil organic matter chemical components (fulvic acid and humic acid) and soil physical fractions. However, there is still limited information about how functional cellulose degradation response to long-term fertilizer management and their relative importance for C sequestration under the double-cropping rice paddy field in southern of China. Therefore, the objective of this study were investigated the effects of 34-years long-term fertilizer regime on community abundance of cbhI and GH48 genes in five soil particle-size fractions (> 2000 μm, 2000–200 μm, 200–50 μm, 50–2 μm and 2–0.1 μm) by using polarization magic angle spinning 13C nuclear magnetic resonance spectroscopy. The field experiment was included four different fertilizer treatments: chemical fertilizer alone (MF), rice straw and chemical fertilizer (RF), 30% organic manure and 70% chemical fertilizer (OM), and without fertilizer input as a control (CK). The results showed that distribution of soil humus and cellulolytic microbial community abundance was significant increased under long-term application of crop residue and organic manure condition. And the FA, HA and HM C contents in > 2000 μm and 2000–50 μm fractions with MF, RF and OM treatments were significant higher than that of CK treatment. Meanwhile, the alkyl C and Oalkyl C groups of FA and HA in > 2000 μm fraction with MF, RF, OM and CK treatments were higher than that of the other fractions. There had higher AL% and lower ARO% of FA and HA in different particle-size fractions with MF, RF, OM and CK treatments. The results indicated that abundance of cbhI and GH48 genes in different particle-size fractions with RF and OM treatments were significant increased, compared with CK treatment. There had significant positive correlation between soil humus C components (FA and HA) with abundance of cbhI and GH48 genes, and the o-alkyl C and AL% of FA were positively correlated with abundance of cbhI and GH48 genes. As a result, the community abundance of cbhI and GH48 genes were significant increased under combined application of crop residue and organic manure with chemical fertilizer condition.

Consequences of the Long-Term Fertilization System Use on Physical and Microbiological Soil Status in the Western Polissia of Ukraine

“…According to our results, greater development of cellulolytic microorganisms was found in both variants with an organic-mineral fertilization system (O1MFS and O2MFS). Other data [77] from a 34-yearlong regime of fertilization experiments also showed a significant increase in the number of cellulolytic microbial communities with the use of crop remains, organic manure, and chemical fertilizers compared with unfertilized soils. Based on this fact, we conclude that in conditions of long-term use of various fertilization systems, the development of cellulolytic organisms increases in the case of a combination of mineral and organic fertilizers, which have a significant content of undecomposed plant remains (straw, siderate).…”

Microbial transformation of soil organic matter under varying agricultural management systems in Ukraine

“…Figures 1 – 3 depict the dynamics of cellulose substrate decomposition in different types of arable soils under various fertilisation systems. Various types of fertilisers had distinct and notable impacts on the activation of cellulolytic microorganisms, which play a key role in breaking down plant-based biopolymers ( Tang et al, 2021 ). Notably, these microorganisms thrived in scenarios where organic fertilisers, such as manure and cover crops, were applied.…”

Nitrapyrin Addition Mitigated CO2 Emission from a Calcareous Soil Was Closely Associated with Its Effect on Decreasing Cellulolytic Fungal Community Diversity

“…Tian et al found that inoculation of cellulolytic fungal increased the abundance of Aspergillus (genus level), thus significantly accelerating the cellulose degradation process. Similarly, Tang et al showed that organic manure combined with rice straw and chemical fertilizers significantly increased cbh I community abundance. Therefore, the cellulolytic fungal glycoside hydrolase family 7 cellobiohydrolase I gene ( cbh I) often acted as a suitable biomarker to explore SOC degradation. ,, Zhang et al found that long-term application of mineral fertilizers together with pig manure significantly increased the abundance of the cbh I cellulolytic fungal gene.…”