Effects of feeding Original XPC™ to broilers with a live coccidiosis vaccine under industrial conditions: Part 2. Cecal microbiota analysis

Park, S. H.; Roto, Stephanie M.; Pavlidis, Hilary O.; McIntyre, D.R.; Striplin, K.; Brammer, Lynnette; Ricke, Steven C.

doi:10.3382/ps/pex014

Cited by 17 publications

(21 citation statements)

References 47 publications

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“…While both methods, DGGE-band sequencing and NGS results were somewhat similar there were differences as well. Such differences in outcomes between DGGE profiles and NGS microbiome sequencing of broiler cecal samples has been observed by our group in previous work [ 27 ]. Therefore, direct comparisons between the two methods must be exercised with caution as they differ considerably in both method and approach as discussed previously by Park et al, 2017 [ 27 ].…”

Section: Resultssupporting

confidence: 65%

“…Such differences in outcomes between DGGE profiles and NGS microbiome sequencing of broiler cecal samples has been observed by our group in previous work [ 27 ]. Therefore, direct comparisons between the two methods must be exercised with caution as they differ considerably in both method and approach as discussed previously by Park et al, 2017 [ 27 ]. Obviously, DGGE-band sequencing relies on visualization of bands and physically excising these bands for sequencing which immediately introduces some operator error.…”

Section: Resultssupporting

confidence: 65%

“…However, DGGE is limited for comprehensive identification of individual organisms in these complex microbial populations making it difficult to detect shifts in microbial groups comprising the range of taxonomic groupings. In-depthtaxonomic identification is now possible with next generation 16S rDNA microbiome sequencing and this approach has been shown to provide more information on cecal microbial populations when compared directly with DGGE analyses in previous studies in our laboratory [ 27 ]. Therefore as a followup analyses to our previous work [ 24 ], the objectives of the current study include not only bird performance assessment but Illumina Miseq 16S rDNA sequencing analysis of the cecal microbiome of conventionally raised broilers fed with commercial prebiotic MB40 compared to negative control group and antibiotic BMD50 fed birds.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of antibiotic supplementation versus a yeast-based prebiotic on the cecal microbiome of commercial broilers

et al. 2017

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Prebiotics are defined as fermentable food ingredients that selectively stimulate beneficial bacteria in the lower gastrointestinal tract of the host. The purpose of this study was to assess growth performance of broilers and the cecal microbial populations of an antibiotic, BMD50, supplemented birds compared to broiler chickens fed the prebiotic, Biolex® MB40. Weight response data including feed conversion ratios (FCR), carcasses without giblets (WOG), wing, skin, white meat were collected during processing. Extracted DNA from cecal contents was utilized for microbiome analysis via an Illumina Miseq. In conclusion, white meat yield of Biolex® MB40 supplemented group exhibited significant improvement compared to both negative control (NC) and BMD50 supplemented groups. In addition, antibiotic significantly decreased level of Lactobacillus in 2 wk compared to other groups. A significantly higher percentage of Campylobacter was observed from the 4 wk old birds treated with antibiotic BMD50 compared to the NC and prebiotic group. Retention of broiler performance and improvement of white meat yield suggest that the prebiotic MB40 appears to be a potential alternative to replace the antibiotic growth promoter.

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Section: Resultssupporting

confidence: 65%

Section: Resultssupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

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Comparison of antibiotic supplementation versus a yeast-based prebiotic on the cecal microbiome of commercial broilers

et al. 2017

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“…Furthermore, several studies ( 25 – 29 ) investigated prebiotic effects on the GIT microbiota through 16S microbiome sequencing. By also noting changes in metabolite concentrations or metabolomics, this approach may be able to correlate changes in the microbiome to changes in the metabolite concentration such as SCFAs and other, possibly unknown, metabolites that can stymie Salmonella growth.…”

Section: Introductionmentioning

confidence: 99%

A Review of Prebiotics Against Salmonella in Poultry: Current and Future Potential for Microbiome Research Applications

et al. 2018

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Prebiotics are typically fermentable feed additives that can directly or indirectly support a healthy intestinal microbiota. Prebiotics have gained increasing attention in the poultry industry as wariness toward antibiotic use has grown in the face of foodborne pathogen drug resistance. Their potential as feed additives to improve growth, promote beneficial gastrointestinal microbiota, and reduce human-associated pathogens, has been well documented. However, their mechanisms remain relatively unknown. Prebiotics increasing short chain fatty acid (SCFA) production in the cecum have long since been considered a potential source for pathogen reduction. It has been previously concluded that prebiotics can improve the safety of poultry products by promoting the overall health and well-being of the bird as well as provide for an intestinal environment that is unfavorable for foodborne pathogens such as Salmonella. To better understand the precise benefit conferred by several prebiotics, “omic” technologies have been suggested and utilized. The data acquired from emerging technologies of microbiomics and metabolomics may be able to generate a more comprehensive detailed understanding of the microbiota and metabolome in the poultry gastrointestinal tract. This understanding, in turn, may allow for improved administration and optimization of prebiotics to prevent foodborne illness as well as elucidate unknown mechanisms of prebiotic actions. This review explores the use of prebiotics in poultry, their impact on gut Salmonella populations, and how utilization of next-generation technologies can elucidate the underlying mechanisms of prebiotics as feed additives.

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“…Feed additives or changes in feed composition have also been shown to confer considerable effects on the gastrointestinal microbiota of poultry and are associated with enhancements in performance parameters (5)(6)(7)(8)(9). Furthermore, antibiotic supplementation has long been known to confer beneficial effects on bird performance and health, but this practice also has an impact on the avian microbiota and the emergence of drug-resistant bacteria (10)(11)(12)(13). This point has resulted in the reduction or elimination of antibiotic use in commercial poultry worldwide.…”

mentioning

confidence: 99%

A Consistent and Predictable Commercial Broiler Chicken Bacterial Microbiota in Antibiotic-Free Production Displays Strong Correlations with Performance

Johnson

Youmans

Noll

et al. 2018

Appl Environ Microbiol

120

192

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Defining the baseline bacterial microbiome is critical to understanding its relationship with health and disease. In broiler chickens, the core microbiome and its possible relationships with health and disease have been difficult to define, due to high variability between birds and flocks. Presented here are data from a large, comprehensive microbiota-based study in commercial broilers. The primary goals of this study included understanding what constitutes the core bacterial microbiota in the broiler gastrointestinal, respiratory, and barn environments; how these core players change across age, geography, and time; and which bacterial taxa correlate with enhanced bird performance in antibiotic-free flocks. Using 2,309 samples from 37 different commercial flocks within a vertically integrated broiler system and metadata from these and an additional 512 flocks within that system, the baseline bacterial microbiota was defined using 16S rRNA gene sequencing. The effects of age, sample type, flock, and successive flock cycles were compared, and results indicate a consistent, predictable, age-dependent bacterial microbiota, irrespective of flock. The tracheal bacterial microbiota of broilers was comprehensively defined, and was the dominant bacterial taxon in the trachea. Numerous bacterial taxa were identified, which were strongly correlated with broiler chicken performance across multiple tissues. While many positively correlated taxa were identified, negatively associated potential pathogens were also identified in the absence of clinical disease, indicating that subclinical dynamics occur that impact performance. Overall, this work provides necessary baseline data for the development of effective antibiotic alternatives, such as probiotics, for sustainable poultry production. Multidrug-resistant bacterial pathogens are perhaps the greatest medical challenge we will face in the 21st century and beyond. Antibiotics are necessary in animal production to treat disease. As such, animal production is a contributor to the problem of antibiotic resistance. Efforts are underway to reduce antibiotic use in animal production. However, we are also challenged to feed the world's increasing population, and sustainable meat production is paramount to providing a safe and quality protein source for human consumption. In the absence of antibiotics, alternative approaches are needed to maintain health and prevent disease, and probiotics have great promise as one such approach. This work paves the way for the development of alternative approaches to raising poultry by increasing our understandings of what defines the poultry microbiome and of how it can potentially be modulated to improve animal health and performance.

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Effects of feeding Original XPC™ to broilers with a live coccidiosis vaccine under industrial conditions: Part 2. Cecal microbiota analysis

Cited by 17 publications

References 47 publications

Comparison of antibiotic supplementation versus a yeast-based prebiotic on the cecal microbiome of commercial broilers

Comparison of antibiotic supplementation versus a yeast-based prebiotic on the cecal microbiome of commercial broilers

A Review of Prebiotics Against Salmonella in Poultry: Current and Future Potential for Microbiome Research Applications

A Consistent and Predictable Commercial Broiler Chicken Bacterial Microbiota in Antibiotic-Free Production Displays Strong Correlations with Performance

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