Effects of feeding of β-carotene-supplemented rotifers on survival and lymphocyte proliferation reaction of fish larvae (Japanese parrotfish (Oplegnathus fasciatus) and Spotted parrotfish(Oplegnathus punctatus)): preliminary trials

The Effects of Different Types of Antioxidants (Se, Vitamin E and Carotenoids) in Broiler Diets on the Growth Performance, Skin Pigmentation and Liver and Plasma Antioxidant Concentrations

“…In other previous animal studies, the survival rates of parrot fish larvae were found to increase with β-carotene supplementation (Tachibana et al 1997), and lycopene was shown to enhance mouse resistance to bacterial infection with Klebsiella pneumoniae (Lingen et al 1959). However, in our study, β-carotene and lycopene supplementation did not improve survival rates.…”

Enhancement of innate immunity in rainbow trout (Oncorhynchus mykiss Walbaum) associated with dietary intake of carotenoids from natural products

“…In studies with mice, carotenoids such as b-carotene, astaxanthin and lutein enhanced antibody production against T-dependent antigens (TD-Ags), their influences being exerted in the initial stages of antigen presentation [26]. Also, Tachibana et al [14] found that lymphocyte proliferation in response to phytohaemagglutinin, a T-cell mitogen, was enhanced by b-carotene, which may explain the high survival of parrotfish larvae observed in that study. In future studies, emphasis should be placed on the measurement of antibody responses to particular antigens/ stimulants to clarify whether carotenoids have indeed any influence on antibody production.…”

“…Thompson et al [15,16] studied vitamin A and astaxanthin as dietary supplements and found that a combination of these did not increase growth or immune indices measured except serum antiprotease activity. On the other hand, spleen lymphocytes of parrotfish showed increased proliferation when stimulated with a mitogen after feeding the larvae with b-carotene enriched rotifers [14]. Also, in our earlier studies, dietary b-carotene and astaxanthin from synthetic sources enhanced certain aspects of the nonspecific defense mechanism in rainbow trout [17,18].…”

“…Already, the effects of carotenoids on aquatic animals are multi-faceted: they enhance larval growth and survival [10], improve the performance of broodstock [11,12] and nauplii quality [13], as well as increase resistance to diseases [14].…”

Effects of light intensity and addition of carotene rich Dunaliella salina live cells on growth and antioxidant activity of Solea senegalensis Kaup (1858) larval and metamorphic stages

“…Many carotenoids serve as effective quenchers of ROS and can prevent lipid peroxidation in marine animals (Miki et al , 1994). Larval parrotfish, Oplegnathus punctatus (Temminck & Schlegel, 1844) and Oplegnathus fasciatus (Temminck & Schlegel, 1844), fed with β‐carotene enriched rotifers, exhibited enhanced survival and increased proliferation of spleen lymphocytes (Tachibana et al , 1997). In the present work, supplementing the artificial food chain generated, in the larval rearing tanks, with β‐carotene rich D. salina cells did not reflect any significant changes on the antioxidant enzymes SOD, KAT and t‐GPX in Senegal sole larvae.…”