Effects of Feeding Management on the Equine Cecal Microbiota

Venable, E. B.; Fenton, Kelby A.; Braner, Victoria M.; Reddington, Catriona E.; Halpin, Michael J.; Heitz, Sarah A.; Francis, Jesse M.; Gulson, N.; Goyer, Cassandra; Bland, Stephanie D.; Cross, Tzu Wen L.; Holscher, Hannah D.; Swanson, Kelly S.

doi:10.1016/j.jevs.2016.09.010

Cited by 49 publications

(37 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Remarkably, the contrast between R and S ponies was also found while comparing their respective gut microbiota composition. Although the constituent phyla and genera within the gut microbiota of R and S ponies were congruent with other studies based on horses (Costa et al, 2012, 2015; Mach et al, 2017; Shepherd et al, 2012; Steelman et al, 2012; Venable et al, 2017; Weese et al, 2015), e.g. members of Firmicutes, Bacteroidetes, Spirochaetes and Fibrobacteres predominating, R ponies presented an increase of several Clostridiales and Bacteroides species at day 24, whereas only species related to Blautia and Paraprevotella genera were relatively more abundant in the S. Indisputably, individuals with different susceptibility to parasite infection adapt to environmental stress in different ways.…”

Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Metagenomic signature of natural strongyle infection in susceptible and resistant horses

Clark

Sallé

Ballan

et al. 2017

Preprint

View full text Add to dashboard Cite

Gastrointestinal strongyles are a major threat to horses' health and welfare. Given that strongyles inhabit the same niche as the gut microbiota, they may interact with each other. These beneficial or detrimental interactions are unknown in horses and could partly explain contrasted susceptibility to infection between individuals. To address these questions, an experimental pasture trial with 20 worm-free female Welsh ponies (10 susceptible (S) and 10 resistant (R) to parasite infection) was implemented for five months. Fecal egg counts (FEC), hematological and biochemical data, body weight and gut microbiota composition were studied in each individual after 0, 24, 43, 92 and 132 grazing days.The predicted R ponies exhibited lower FEC after 92 and 132 grazing days, and showed higher levels of circulating monocytes and eosinophils, while S ponies developed lymphocytosis by the end of the trial. Although the overall microbiota diversity remained similar between the two groups, R and S ponies exhibited sustained differential abundances in Clostridium XIVa, Ruminococcus, Acetivibrio and unclassified Lachnospiracea at day 0. These bacteria may hence contribute to the intrinsic pony resistance towards strongyle infection. Moreover, Paludibacter, Campylobacter, Bacillus, Pseudomonas, Clostridium III, Acetivibrio, members of the unclassified Eubacteriaceae and Ruminococcaceae and fungi loads were increased in infected S ponies, suggesting that strongyle and fungi may contribute to each other’s success in the ecological niche of the equine intestines. In contrast, butyrate-producing bacteria such as Ruminococcus, Clostridium XIVa and members of the Lachnospiraceae family decreased in S relative to R ponies. Additionally, these gut microbiota alterations induced changes in several immunological pathways in S ponies, including pathogen sensing, lipid metabolism, and activation of signal transduction that are critical for the regulation of immune system and energy homeostasis. These observations shed light on a putative implication of the gut microbiota in the intrinsic resistance to strongyle infection.Overall, this longitudinal study provides a foundation to better understand the mechanisms that underpin the relationship between host susceptibility to strongyle infection, immune response and gut microbiota under natural conditions in horses and should contribute to the development of novel biomarkers of strongyle susceptibility and provide additional control options.

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Metagenomic signature of natural strongyle infection in susceptible and resistant horses

Clark

Sallé

Ballan

et al. 2017

Preprint

View full text Add to dashboard Cite

show abstract

“…Fecal DNA was extracted according to manufacturer’s guidelines using the MoBio Powerlyzer Powersoil Kit (MoBIO Laboratories Inc., Carlsbad, CA, USA). After extraction, sequencing was performed at the W. M. Keck Center for Biotechnology at the University of Illinois as previously described [ 24 ]. Briefly, bacterial (V4 region of 16S rRNA; 515F/806R: 5′-GTGCCAGCMGCCGCGGTAA, 5′-GGACTACHVGGGTWTCTAAT), archaeal (ArchaeaF/ArchaeaR: 5′-GYGCASCAGKCGMGAAW, 5′-GGACTACVSGGGTATCTAAT) and fungal (ITS1F/ITS4R: 5′-TTCGTAGGTGAACCTGCGG, 5′-TCCTCCGCTTATTGATATGC) genes were amplified using a Fluidigm access array (Fluidigm, South San Francisco, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Almond Consumption and Processing Affects the Composition of the Gastrointestinal Microbiota of Healthy Adult Men and Women: A Randomized Controlled Trial

et al. 2018

View full text Add to dashboard Cite

Background: Almond processing has been shown to differentially impact metabolizable energy; however, the effect of food form on the gastrointestinal microbiota is under-investigated. Objective: We aimed to assess the interrelationship of almond consumption and processing on the gastrointestinal microbiota. Design: A controlled-feeding, randomized, five-period, crossover study with washouts between diet periods was conducted in healthy adults (n = 18). Treatments included: (1) zero servings/day of almonds (control); (2) 1.5 servings (42 g)/day of whole almonds; (3) 1.5 servings/day of whole, roasted almonds; (4) 1.5 servings/day of roasted, chopped almonds; and (5) 1.5 servings/day of almond butter. Fecal samples were collected at the end of each three-week diet period. Results: Almond consumption increased the relative abundances of Lachnospira, Roseburia, and Dialister (p ≤ 0.05). Comparisons between control and the four almond treatments revealed that chopped almonds increased Lachnospira, Roseburia, and Oscillospira compared to control (p < 0.05), while whole almonds increased Dialister compared to control (p = 0.007). There were no differences between almond butter and control. Conclusions: These results reveal that almond consumption induced changes in the microbial community composition of the human gastrointestinal microbiota. Furthermore, the degree of almond processing (e.g., roasting, chopping, and grinding into butter) differentially impacted the relative abundances of bacterial genera.

show abstract

“…Equine strongyles are in close contact with a large community of microorganisms in the host intestines, estimated to reach a concentration of 10 9 microorganisms per gram of ingesta in the cecum alone (Mackie and Wilkins, 1988 ), spanning 108 bacterial genera (Steelman et al, 2012 ; Mach et al, 2017 ; Venable et al, 2017 ) and at least seven phyla (Costa et al, 2012 , 2015 ; Shepherd et al, 2012 ; Weese et al, 2015 ; Mach et al, 2017 ). Bacterial populations differ greatly throughout the various compartments of the equine gastrointestinal tract (e.g., duodenum, jejunum, ileum and colon) due to differences in the gut pH, available energy sources, epithelial architecture of each region, oxygen levels and physiological roles (Costa et al, 2015 ; Ericsson et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Strongyle Infection and Gut Microbiota: Profiling of Resistant and Susceptible Horses Over a Grazing Season

et al. 2018

View full text Add to dashboard Cite

Gastrointestinal strongyles are a major threat to horses' health and welfare. Given that strongyles inhabit the same niche as the gut microbiota, they may interact with each other. These beneficial or detrimental interactions are unknown in horses and could partly explain contrasted susceptibility to infection between individuals. To address these questions, an experimental pasture trial with 20 worm-free female Welsh ponies (10 susceptible (S) and 10 resistant (R) to parasite infection) was implemented for 5 months. Fecal egg counts (FEC), hematological and biochemical data, body weight and gut microbiological composition were studied in each individual after 0, 24, 43, 92 and 132 grazing days. R and S ponies displayed divergent immunological profiles and slight differences in microbiological composition under worm-free conditions. After exposure to natural infection, the predicted R ponies exhibited lower FEC after 92 and 132 grazing days, and maintained higher levels of circulating monocytes and eosinophils, while lymphocytosis persisted in S ponies. Although the overall gut microbiota diversity and structure remained similar during the parasite infection between the two groups, S ponies exhibited a reduction of bacteria such as Ruminococcus, Clostridium XIVa and members of the Lachnospiraceae family, which may have promoted a disruption of mucosal homeostasis at day 92. In line with this hypothesis, an increase in pathobionts such as Pseudomonas and Campylobacter together with changes in several predicted immunological pathways, including pathogen sensing, lipid metabolism, and activation of signal transduction that are critical for the regulation of immune system and energy homeostasis were observed in S relative to R ponies. Moreover, S ponies displayed an increase in protozoan concentrations at day 92, suggesting that strongyles and protozoa may contribute to each other's success in the equine intestines. It could also be that S individuals favor the increase of these carbohydrate-degrading microorganisms to enhance the supply of nutrients needed to fight strongyle infection. Overall, this study provides a foundation to better understand the mechanisms that underpin the relationship between equines and natural strongyle infection. The profiling of horse immune response and gut microbiota should contribute to the development of novel biomarkers for strongyle infection.

show abstract

Effects of Feeding Management on the Equine Cecal Microbiota

Cited by 49 publications

References 37 publications

Metagenomic signature of natural strongyle infection in susceptible and resistant horses

Metagenomic signature of natural strongyle infection in susceptible and resistant horses

Almond Consumption and Processing Affects the Composition of the Gastrointestinal Microbiota of Healthy Adult Men and Women: A Randomized Controlled Trial

Strongyle Infection and Gut Microbiota: Profiling of Resistant and Susceptible Horses Over a Grazing Season

Contact Info

Product

Resources

About