Effects of fatty acid salts on fermentation characteristics, bacterial diversity and aerobic stability of mixed silage prepared with alfalfa, rice straw and wheat bran

Mu, Lin; Wang, Qinglan; Cao, Xiaoyan; Zhang, Zhifei

doi:10.1002/jsfa.11482

Cited by 20 publications

(20 citation statements)

References 42 publications

(85 reference statements)

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“…L. parabrevis was also found to have a positive relation with AA. Mu et al reported that the increase of LA and AA in silages was closely related to L. parabrevis [ 63 ]. In addition, Le.…”

Section: Discussionmentioning

confidence: 99%

Effects of Malic Acid and Sucrose on the Fermentation Parameters, CNCPS Nitrogen Fractions, and Bacterial Community of Moringa oleifera Leaves Silage

et al. 2021

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The present study investigated the effects of malic acid, sucrose, and their mixture on the fermentation parameters, Cornell Net Carbohydrate and Protein System (CNCPS) nitrogen fractions, and bacterial community of Moringa oleifera leaves (MOL) silages. The trial was divided into four treatments and labeled as CON (control group) and MLA, SUC, and MIX (respectively denoting the addition of 1% malic acid, 1% sucrose, and 1% malic acid + 1% sucrose to the fresh weight basis). The silage packages were opened on the 2nd, 5th, 10th, 20th, and 40th days of ensiling for subsequent determination. Malic acid and sucrose increased the lactic acid content (p < 0.05) and pH value, and the acetic acid contents of MLA and MIX were lower than those in CON (p < 0.05). Compared with sucrose, malic acid had a better capacity to preserve nutrients and inhibit proteolysis, and thus exerted better effects on the CNCPS nitrogen fractions. The results of 16S rRNA showed that the dominant phyla were Firmicutes and Proteobacteria and that the dominant genera were Lactobacillus and Weissella. With the application of silage additives and the processing of fermentation, there was a remarkable change in the composition and function of the bacterial community. The variation of the fermentation parameters and CNCPS nitrogen fractions in the MOL silages caused by malic acid and sucrose might be attributed to the dynamic and dramatic changes of the bacterial community.

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“…L. parabrevis was also found to have a positive relation with AA. Mu et al reported that the increase of LA and AA in silages was closely related to L. parabrevis [ 63 ]. In addition, Le.…”

Section: Discussionmentioning

confidence: 99%

Effects of Malic Acid and Sucrose on the Fermentation Parameters, CNCPS Nitrogen Fractions, and Bacterial Community of Moringa oleifera Leaves Silage

et al. 2021

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“…In addition, the WSC content of J silage decreased more rapidly compared to that of other treatments during 5 days of ensiling, perhaps due to this silage having more LA and AA. Similarly, lower WSC in calcium propionate-treated silage after 45 days of ensiling was attributed to higher LA and AA ( Mu et al, 2021 ). Concentration of CP was higher in J silage than C silage on Day 45.…”

Section: Discussionmentioning

confidence: 99%

“…The DNA was quantified with a NanoDrop 2000 and quality determined with 1% agarose gels. Single-molecule real-time (SMRT) sequencing was done, with primers 27F and 1492R to detect 16S rRNA genes, and polymerase chain reaction (PCR) was done as described by Mu et al (2021) . All DNA assessments were done by Biomarker Technologies Corporation (Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

“…All DNA assessments were done by Biomarker Technologies Corporation (Beijing, China). A PacBio Sequel (Pacific Biosciences, Menlo Park, CA) was used for analyses, with sequences determined as described by Mu et al (2021) . Alpha diversity used Shannon, Simpson’s diversity, Chao1 and rarefaction estimators, principle component analysis (PCA), R heatmaps were prepared as described by Mu et al (2020) , and Venn diagrams (VennDiagram, Version 1.6.16) were used to compare bacterial communities.…”

Section: Methodsmentioning

confidence: 99%

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The Potential of Pre-fermented Juice or Lactobacillus Inoculants to Improve the Fermentation Quality of Mixed Silage of Agro-Residue and Lucerne

Wang

Cao

et al. 2022

Front. Microbiol.

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The objective of this study was to determine the effect of pre-fermented juice, Lactobacillus plantarum, and L. buchneri on chemical composition, fermentation, aerobic stability, dynamics of microbial community, and metabolic pathway of a mixture of lucerne, wheat bran (WB), and rice straw (RS). All mixtures were ensiled for 1, 3, 5, 7, 15, 30, and 45 days after treatment with uninoculated (control, C); L. plantarum [LP, 1 × 106 cfu/g of fresh weight (FW)]; L. buchneri (LB, 1 × 106 cfu/g of FW); LP + LB (LPB, 1 × 106 cfu/g of FW of each inoculant); and pre-fermented juice (J; 2 × 106 cfu/g of FW). Four lactic acid bacteria (LAB) species from three genera were cultured from the pre-fermented juice, with W. cibaria being dominant. The inoculants increased lactic acid (LA), decreased pH and ammonia nitrogen (AN) compared to C silage at earlier stages of ensiling, and high dry matter (DM) and water-soluble carbohydrate (WSC) content in inoculated silages. Adding LPB increased the abundance of L. plantarum, L. paralimentarius, and L. nodensis, resulting in the lowest pH. Pre-fermented juice enriched W. cibaria, L. sakei, L. parabrevis, Pseudomonas putida, and Stenotrophomonas maltophilia, mainly enhanced accumulation of acetic acid (AA) and LA, and decreased pH, crude protein losses, AN, and hemicellulose contents. L. buchneri and L. brevis had a high abundance in LB-treated and J silages, respectively, inhibited undesirable bacteria, and improved aerobic stability with more than 16 days. In addition, the metabolic pathways changed with time and L. buchneri inoculants promoted global metabolism. In conclusion, inoculations altered bacterial succession and metabolic pathways in silage; LB and pre-fermented juice enhanced ensiling by promoting pH reductions, enhancing concentrations of LA and AA, and extending aerobic stability more than 16 days.

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“…The chemical pretreatment of RH showed a 32% increase in biogas yield compared to untreated RH in the case of mono-digestion. Mu et al (2021) also checked the effect of acid mixtures like acetic and propionic acid but do not measure any variance in the treated and untreated RH. In another study by Dahunsi (2019) , the pretreatment with 2% sulfuric acid may reduce the hemicellulose content by 69%.…”

Section: Discussionmentioning

confidence: 99%

Microbial Pretreatment of Chicken Feather and Its Co-digestion With Rice Husk and Green Grocery Waste for Enhanced Biogas Production

et al. 2022

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To utilize wastes and residues sustainably and excellently, there is a need to fend for efficient methods and resources for biogas production. Use of poultry waste for biogas production represents one of the most important routes toward reaching global renewable energy targets. The current study involves microbial pretreatment of chicken feather waste, followed by its co-digestion with rice husk and green grocery waste in batch and continuous reactors, respectively. Microbial pretreatment of chicken feathers by keratinase secreting Pseudomonas aeruginosa was an effective and eco-friendly approach to make its recalcitrant structure available as a raw substrate for biogas production. The current study also addressed the enhancement and stability of anaerobic digestion by co-digestion. Results demonstrated that biogas production was increased by microbial pretreatment of chicken feathers and that the percentage increase in biogas yield was 1.1% in microbialy pretreated feathers compared to mono-digestion (non-pretreated feathers) in batch fermentation. The highest yield of biogas was obtained in a batch reactor having co-digestion of pretreated rice husk and microbial pretreated chicken feathers. The co-digestion of chicken feathers hydrolysate with green grocery waste in continuous fermentation mode has also enhanced the biogas yield as compared to average of mono-digestion (chicken feather hydrolysate and green grocery waste) and, therefore, improve the efficiency of the overall process.

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Effects of fatty acid salts on fermentation characteristics, bacterial diversity and aerobic stability of mixed silage prepared with alfalfa, rice straw and wheat bran

Cited by 20 publications

References 42 publications

Effects of Malic Acid and Sucrose on the Fermentation Parameters, CNCPS Nitrogen Fractions, and Bacterial Community of Moringa oleifera Leaves Silage

Effects of Malic Acid and Sucrose on the Fermentation Parameters, CNCPS Nitrogen Fractions, and Bacterial Community of Moringa oleifera Leaves Silage

The Potential of Pre-fermented Juice or Lactobacillus Inoculants to Improve the Fermentation Quality of Mixed Silage of Agro-Residue and Lucerne

Microbial Pretreatment of Chicken Feather and Its Co-digestion With Rice Husk and Green Grocery Waste for Enhanced Biogas Production

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