Effects of far red light on the induction changes of prompt and delayed fluorescence and the redox state of P700 in Scenedesmus quadricauda

Lenbaum, V. V.; Булычев, А. А.; Matorin, D. N.

doi:10.1134/s1021443715020132

Cited by 5 publications

(2 citation statements)

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“…The average chlorophyll content in the cells pelleted on filters was ~400 mg/m 2 , which was comparable to its content in leaves of vascular plants (approximately 500 mg/m 2 ) [10]. Control measurements of fluorescence on cell suspensions of Scenedesmus quadricauda using an Aqua-Pen-C (AP-C 100) fluorometer (Photon Systems Instruments, Czech Republic) have shown that the procedure of concen-trating samples on filters had no influence on cell physiological condition [9].…”

Section: Methodsmentioning

confidence: 87%

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Pigment mutants of the green microalga Chlamydomonas reinhardtii: Morphological properties and photosynthetic performance

Sadvakasova

Акмуханова

Zayadan

et al. 2016

Russ J Plant Physiol

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Biodiesel is an alternative energy source which has attracted increasing attention lately. Although algae-based biodiesel production has many benefits, it is still far from industrial application. Research suggests that improving lipid quality and production through genetic engineering of metabolic pathways will be the most promising way. To enhance lipid content, both lysophosphatidic acyltransferase gene (c-lpaat) and glycerol-3-phosphate dehydrogenase gene (c-gpd1), optimized according to the codon bias of Chlamydomonas reinhardtii, were inserted into the genomic DNA of model microalga C. reinhardtii by the glass bead method. Transgenic algae were screened by zeomycin resistance and RT-PCR. The transcription levels of inserted genes and the fatty acid content were significantly increased after intermittent heat shock. Most of all, the transcription levels of c-lpaat and c-gpd1 were increased 5.3 and 8.6 times after triple heat shocks, resulting in an increase of 44.5 and 67.5% lipid content, respectively. Furthermore, the content of long-chain saturated fatty acids and monounsaturated fatty acids, especially C18 and C18:1t, notably increased, while unsaturated fatty acids dramatically decreased. The results of this study offer a new strategy combining genetic manipulation and intermittent heat shock to enhance lipid production, especially the production of long-chain saturated fatty acids, using C. reinhardtii.

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Section: Methodsmentioning

confidence: 87%

“…The duration of each measurement was 60 s. The DF kinetics reflected changes in emission intensity on a time scale of 0.1-0.9 ms in the intervals between actinic light pulses. Characteristics and the protocol of measurements with an M-PEA-2 instrument have been described in detail previously [6,8,9].…”

Section: Methodsmentioning

confidence: 99%