Effects of Extraction Methods and Conditions on Bioactive Compounds Extracted from Phaeodactylum tricornutum

Abstract

The effect of homogenization, ultrasound and microwave extraction methods and conditions on fucoxanthin content, total phenolic content and antioxidant activity of extracts obtained from Phaeodactylum tricornutum were investigated in this study. The solvent/biomass ratio was the most effective parameter on fucoxanthin content, total phenolic content and antioxidant activity. The maximum fucoxanthin content (5.60 ± 0.06 mg/g) and antioxidant activity (763.00 ± 15.88 EC50 μg/mL extract) were obtained with the homogenization extraction method whose optimum conditions were 1.93% biomass/solvent ratio, ~5200 rpm homogenization rate and 14.2 min extraction time. Although the ultrasonic extraction method has reached the approximately same level of fucoxanthin content (5.24 ± 0.07 mg/g)), TPC (67.68 ± 1.58 mg gallic acid/L) and antioxidant activity (619.90 ± 17.16 EC50 μg/mL extract) at an amplitude of 55.72%, a higher biomass/solvent ratio (2.72%) and a longer extraction time (17.37 min) have been required. The lowest fucoxanthin content, total phenolic content and antioxidant activity were determined for the microwave extraction method.

Ultrasound Extraction Mediated Recovery of Nutrients and Antioxidant Bioactive Compounds from Phaeodactylum tricornutum Microalgae

“…This can be explained by the increase in the antioxidant compounds´ extraction with the passage of time. Akyıl et al [34] also found a significant influence of the extraction time on the antioxidant activity, measured by DPPH, when they optimized the bioactive compound extraction from P. tricornutum. However, Kokkali et al [15] found a minimal decrease in antioxidant activity, measured by ABTS in P. tricornutum aqueous extracts, comparing 4 h and 24 h of extraction.…”

Optimization of Extraction Conditions of Bioactive Compounds by Ultrasonic-Assisted Extraction from Artichoke Wastes

“…Moreover, it is vital to determine the suitable extraction method of the bioactive compounds from plants in terms of extraction yield. 16 Most of the industrial applications have tended towards green technologies. Hence, the techniques for the extraction of polyphenols from food wastes should also be innovative and environmentally friendly.…”