Effects of external radiation in a co-culture model of endothelial cells and adipose-derived stem cells

Haubner, Frank; Leyh, Michaela; Ohmann, Elisabeth; Pohl, Fabian; Prantl, Lukas; Gassner, Holger G.

doi:10.1186/1748-717x-8-66

Cited by 37 publications

(47 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Die zellbiologischen Interaktionen von Fettstammzellen mit den relevanten Zellarten der Wundheilung wurden in der Vergangenheit u. a. von der Arbeitsgruppe der Autoren untersucht [13]. Hier wurde gezeigt, dass die direkte Interaktion von Fettstammzellen mit Endothelzellen und Fibroblasten einen modulierenden Effekt auf die Entzündungsreaktion nach externer Bestrahlung ausübt.…”

Section: Rolle Adipogener Stammzellenunclassified

“…Der Anstieg wundheilungsrelevanter und proinflammatorischer Marker nach externer Bestrahlung von Fibroblasten und Endothelzellen scheint durch die Kokultur mit adipogenen Stammzellen moduliert zu werden. Auch die Marker der endothelialen Dysfunktion ICAM und VCAM konnten in vitro durch adipogene Stammzellen moduliert werden [13]. Die vorliegenden In-vitro-Daten unterstützen die Hypothese, dass adipogene Stammzellen positive Effekte auf bestrahlte Wunden ausüben könnten [13].…”

Section: Rolle Adipogener Stammzellenunclassified

“…Auch die Marker der endothelialen Dysfunktion ICAM und VCAM konnten in vitro durch adipogene Stammzellen moduliert werden [13]. Die vorliegenden In-vitro-Daten unterstützen die Hypothese, dass adipogene Stammzellen positive Effekte auf bestrahlte Wunden ausüben könnten [13].…”

Section: Rolle Adipogener Stammzellenunclassified

“…Dies kann zu einer anhaltenden entzündlichen Gewebeverän-derung mit dem Auftreten chronischer Wunden führen. Anhand von Zellkulturmodellen konnte die Arbeitsgruppe der Autoren die proinflammatorischen Zellreaktionen nach externer Bestrahlung dokumentieren [12,13]. Dies spiegelt sich im Anstieg von IL-6 nach externer Bestrahlung von dermalen Fibroblasten und mikrovaskulären Endothelzellen wider (.…”

Section: Introductionunclassified

See 3 more Smart Citations

Potenzial adipogener Stammzellen bei radiogenen Wundheilungsstörungen

Haubner

Gassner

2015

HNO

View full text Add to dashboard Cite

show abstract

Section: Rolle Adipogener Stammzellenunclassified

Section: Introductionunclassified

See 2 more Smart Citations

Potenzial adipogener Stammzellen bei radiogenen Wundheilungsstörungen

Haubner

Gassner

2015

HNO

View full text Add to dashboard Cite

show abstract

“…Smith et al (28) investigated that dermal fibroblasts down-regulate expression of ICAM1, VCAM1, and MMP11 in response to MSCs and suggested that downregulation of ICAM1 expression by dermal fibroblasts may be necessary for resolution of inflammation during wound repair. Haubner et al (29) found the increased expression of cytokines and adhesion molecules as ICAM-1 by human dermal microvascular endothelial cells after external radiation is mitigated in the co-culture setting with human adipose-derived stem Integrins are the main cell surface receptors for proteins within the extracellular matrix (ECM). They underpin cell adhesion and migration and are also involved in cell proliferation, programmed cell death, and differentiation (30).…”

Section: Results Of Wound Healing According To Mcroscopic Signs Aftermentioning

confidence: 99%

The role of placenta‐derived mesenchymal stem cells in healing of induced full‐thickness skin wound in a mouse model

et al. 2015

View full text Add to dashboard Cite

We examined the effect of placenta-derived MSCs (PDMSCs) injection intraregionally and intraperitoneally on healing of induced full thickness mice skin wounds; moreover, the mechanisms by which MSCs exert their effects were also studied. Sixty female mice were divided into three groups after induction of full thickness skin wound; untreated group, wounded mice were injected with MSCs derived from human placenta intraperitoneally or intraregionally. Skin biopsies were obtained 7 and 12 days after wound incision for histological examinations, detection of vascular endothelial growth factor (VEGF) by ELISA, and estimation of expression of mouse ICAM-1, Integrin b1, Integrin b3 genes and human albumin and GAPDH genes by reverse transcription polymerase chain reaction. Human placenta derived-MSCs treated groups showed accelerated wound healing than non-treated group. VEGF, Integrin b1, and Integrin b3 levels were significantly increased in the intraregionally and intraperitoneally treated mice as compared to non-treated group at day 7 after wound induction. ICAM-1 showed significant decrease in its expression in treated groups compared with non-treated group. Interestingly, the intraperitoneal MSCs injections showed better results than intraregional one. PDMSCs accelerate full thickness skin wound healing and the intraperitoneal MSCs injections are more effective than intraregional one. MSCs promote wound healing through release of proangiogenic factors as VEGF, increase healing promoting factors as integrin b1 and b3, and decrease proinflammatory cytokines as ICAM-1. V C 2015 IUBMB Life, 67(9): [701][702][703][704][705][706][707][708][709] 2015

show abstract

Molecular Characterization of Lipoaspirates Used in Regenerative Head and Neck Surgery

Sharaf

Kleinsasser

Schwenk‐Zieger

et al. 2019

JAMA Facial Plast Surg

Self Cite

View full text Add to dashboard Cite

IMPORTANCE Adipose-derived mesenchymal stem cells (ASCs) have been used commonly in regenerative medicine and increasingly for head and neck surgical procedures. Lipoaspiration with centrifugation is purported to be a mild method for the extraction of ASCs used for autologous transplants to restore tissue defects or induce wound healing. The content of ASCs, their paracrine potential, and cellular potential in wound healing have not been explored for this method to our knowledge. OBJECTIVE To evaluate the characteristics of lipoaspirates used in reconstructive head and neck surgical procedures with respect to wound healing. DESIGN, SETTING, AND PARTICIPANTSThis case series study included 15 patients who received autologous fat injections in the head and neck during surgical procedures at a tertiary referral center. The study was performed from October 2017 to November 2018, and data were analyzed from October 2017 to February 2019. MAIN OUTCOMES AND MEASURESExcessive material of lipoaspirates from subcutaneous abdominal fatty tissue was examined. Cellular composition was analyzed using immunohistochemistry (IHC) and flow cytometry, and functionality was assessed through adipose, osteous, and chondral differentiation in vitro. Supernatants were tested for paracrine ASC functions in fibroblast wound-healing assays. Enzyme-linked immunosorbent assay measurement of tumor necrosis factor (TNF), vascular endothelial growth factor (VEGF), stromal-derived factor 1α (SDF-1α), and transforming growth factor β3 (TGF-β3) was performed.RESULTS Among the 15 study patients (8 [53.3%] male; mean [SD] age at the time of surgery, 63.0 [2.8] years), the stromal vascular fraction (mean [SE], 53.3% [4.2%]) represented the largest fraction within the native lipoaspirates. The cultivated cells were positive for CD73 (mean [SE], 99.90% [0.07%]), CD90 (99.40% [0.32%]), and CD105 (88.54% [2.74%]); negative for CD34 (2.70% [0.45%]) and CD45 (1.74% [0.28%]) in flow cytometry; and negative for CD14 (10.56 [2.81] per 300 IHC score) and per 300 IHC score) in IHC staining; they differentiated into osteoblasts, adipocytes, and chondrocytes. The cultivated cells showed high expression of CD44 (mean [SE], 99.78% [0.08%]) and CD273 (82.56% [5.83%]). The supernatants were negative for TNF (not detectable) and SDF-1α (not detectable) and were positive for VEGF (mean [SE], 526.74 [149.84] pg/mL for explant supernatants; 528.26 [131.79] pg/10 6 per day for cell culture supernatants) and TGF-β3 (mean [SE], 22.79 [3.49] pg/mL for explant supernatants; 7.97 [3.15] pg/10 6 per day for cell culture supernatants). Compared with control (25% or 50% mesenchymal stem cell medium), fibroblasts treated with ASC supernatant healed the scratch-induced wound faster (mean [SE]: control, 1.000 [0.160]; explant supernatant, 1.369 [0.070]; and passage 6 supernatant, 1.492 [0.094]). CONCLUSIONS AND RELEVANCEThe cells fulfilled the international accepted criteria for mesenchymal stem cells. The lipoaspirates contained ASCs that had the potential to multidifferentiate w...

show abstract

Effects of external radiation in a co-culture model of endothelial cells and adipose-derived stem cells

Cited by 37 publications

References 26 publications

Potenzial adipogener Stammzellen bei radiogenen Wundheilungsstörungen

Potenzial adipogener Stammzellen bei radiogenen Wundheilungsstörungen

The role of placenta‐derived mesenchymal stem cells in healing of induced full‐thickness skin wound in a mouse model

Molecular Characterization of Lipoaspirates Used in Regenerative Head and Neck Surgery

Contact Info

Product

Resources

About