Potenzial adipogener Stammzellen bei radiogenen Wundheilungsstörungen

Haubner, Frank; Gassner, Holger G.

doi:10.1007/s00106-014-2953-y

Cited by 7 publications

(4 citation statements)

References 33 publications

(29 reference statements)

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“…In addition, we observed increased amounts of VEGF after external radiation of NHF and ASC mono-cultures in previous studies [ 28 ]. Irradiation and surgery are well known to induce hypoxia [ 32 ] which is a relevant factor in inducing VEGF transcription [ 33 ].…”

Section: Resultssupporting

confidence: 63%

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A Co-Culture Model of Fibroblasts and Adipose Tissue-Derived Stem Cells Reveals New Insights into Impaired Wound Healing After Radiotherapy

Haubner¹,

Pohl

Schreml

et al. 2015

IJMS

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External radiation seems to be associated with increased amounts of cytokines and other cellular modulators. Impaired microcirculation and fibrosis are examples of typical long term damage caused by radiotherapy. Adipose tissue-derived stem cells (ASC) are discussed to enhance wound healing, but their role in wounds due to radiotherapy is poorly understood. Normal human fibroblasts (NHF) and ASCs were co-cultured and external radiation with doses from 2–12 Gray (Gy) was delivered. Cell proliferation and mRNA levels of matrix metalloproteinases (MMP1, MMP2 and MMP13) were determined 48 h after irradiation of the co-cultures by qPCR. Additionally, tissue inhibitors of matrix metalloproteinases (TIMP1, TIMP2) were determined by enzyme-linked immunosorbent assay (ELISA). There was a reduction of cell proliferation after external radiation in mono-cultures of NHFs and ASCs compared to controls without irradiation. The co-culture of ASCs and NHFs showed reduced impairment of cell proliferation after external radiation. Gene expression of MMP1 and MMP13 was reduced after external irradiation in NHF. MMP2 expression of irradiated NHFs was increased. In the co-culture setting, MMP1 and MMP2 gene expression levels were upregulated. TIMP1 and TIMP2 protein expression was increased after irradiation in NHFs and their co-cultures with ASCs. ASCs seem to stimulate cell proliferation of NHFs and modulate relevant soluble mediators as well as proteinases after external radiation.

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Section: Resultssupporting

confidence: 63%

“…Comparable to the effect of external radiation on HDMECs, we found modulatory effects of ASCs concerning the IL-6 expression in NHFs [ 28 ]. IL-6 is a potent inducer of MMP1 and TIMP1 in dermal fibroblasts [ 29 ] and has also been reported to increase MMP13 expression in human chondrosarcoma cells by Tang et al [ 30 ].…”

Section: Resultsmentioning

confidence: 98%

A Co-Culture Model of Fibroblasts and Adipose Tissue-Derived Stem Cells Reveals New Insights into Impaired Wound Healing After Radiotherapy

Haubner¹,

Pohl

Schreml

et al. 2015

IJMS

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“…Alone, or combined with scaffold, ADSCs have proven efficiency in restoring maxillary defects, accompanied with apparent bone regeneration [164]. Post-traumatic calvarial defects were also treated with autologous ADSCs and calvarial continuity with bone regeneration were obtained [165]. Their ability to secrete anti-inflammatory factors and decrease pro-inflammatory responses also makes them very attractive and efficient in the treatment of inter-vertebral disc damages, rheumatoid arthritis, osteoarthritis, and tendon damages [19,22,150,151,152,153,154].…”

Section: Current Approaches In Adipose Derived Stem Cells (Adscs)-mentioning

confidence: 99%

Regenerative Capacity of Adipose Derived Stem Cells (ADSCs), Comparison with Mesenchymal Stem Cells (MSCs)

Mazini

Rochette

Amine

et al. 2019

IJMS

297

212

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Adipose tissue is now on the top one of stem cell sources regarding its accessibility, abundance, and less painful collection procedure when compared to other sources. The adipose derived stem cells (ADSCs) that it contains can be maintained and expanded in culture for long periods of time without losing their differentiation capacity, leading to large cell quantities being increasingly used in cell therapy purposes. Many reports showed that ADSCs-based cell therapy products demonstrated optimal efficacy and efficiency in some clinical indications for both autologous and allogeneic purposes, hence becoming considered as potential tools for replacing, repairing, and regenerating dead or damaged cells. In this review, we analyzed the therapeutic advancement of ADSCs in comparison to bone marrow (BM) and umbilical cord (UC)-mesenchymal stem cells (MSCs) and designed the specific requirements to their best clinical practices and safety. Our analysis was focused on the ADSCs, rather than the whole stromal vascular fraction (SVF) cell populations, to facilitate characterization that is related to their source of origins. Clinical outcomes improvement suggested that these cells hold great promise in stem cell-based therapies in neurodegenerative, cardiovascular, and auto-immunes diseases.

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“…Mesenchymal stem cells are suggested to support wound healing due to their production of multiple growth factors and cytokines which are of major interest in wound healing processes [ 4 ]. The discovery of adipose-derived stem cells (ASC) also as multipotent progenitors of various cell types was an important step forward in the field of stem cell research [ 5 , 6 ]. Furthermore, ASC can be obtained easily by rather low invasive procedures such as liposuction.…”

Section: Introductionmentioning

confidence: 99%

Impact of Platelet-Rich Plasma on Viability and Proliferation in Wound Healing Processes after External Radiation

Reinders

Felthaus

Brockhoff

et al. 2017

IJMS

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Platelet-rich plasma is a current subject of studies on chronic wound healing therapy due to possible pro-angiogenic effects. Microvascular compromise represents the major component in radiogenic wound healing complications. The effects of platelet-rich plasma on irradiated cells of the cutaneous wound healing process are poorly understood so far. In this study, the interaction of endothelial cells and adipose-derived stem cells in conjunction with treatment with platelet-rich plasma is investigated in the context of radiation effects. Therefore, the expression of surface-marker CD90 and CD31 was determined. Moreover, cell proliferation and viability after external radiation was analyzed with and without treatment by platelet-rich plasma.

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Potenzial adipogener Stammzellen bei radiogenen Wundheilungsstörungen

Cited by 7 publications

References 33 publications

A Co-Culture Model of Fibroblasts and Adipose Tissue-Derived Stem Cells Reveals New Insights into Impaired Wound Healing After Radiotherapy

A Co-Culture Model of Fibroblasts and Adipose Tissue-Derived Stem Cells Reveals New Insights into Impaired Wound Healing After Radiotherapy

Regenerative Capacity of Adipose Derived Stem Cells (ADSCs), Comparison with Mesenchymal Stem Cells (MSCs)

Impact of Platelet-Rich Plasma on Viability and Proliferation in Wound Healing Processes after External Radiation

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