Effects of exposure to quaternary-ammonium-based biocides on antimicrobial susceptibility and tolerance to physical stresses in bacteria from organic foods

Gadea, Rebeca; Fuentes, Miguel Ángel Fernández; Pulido, Rubén Pérez; Gálvez, Antonio; Ortega, Elena

doi:10.1016/j.fm.2016.10.037

Cited by 76 publications

(54 citation statements)

References 71 publications

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“…The effect of biocidal agents used for disinfection to enhance cross-resistance to antibiotics has been highlighted in different reports. In the case of K. pneumoniae, occurrence of resistance to different antibiotics, including colistin, has been revealed after exposure to benzalkonium chloride [66] and chlorhexidine digluconate [67,68]. Therefore, we have also followed the distribution of disinfectants resistance genes in the analyzed strains.…”

Section: Discussionmentioning

confidence: 99%

Whole genome sequencing snapshot of multi-drug resistant Klebsiella pneumoniae strains from hospitals and receiving wastewater treatment plants in Southern Romania

Surleac

Barbu

Paraschiv³

et al. 2020

PLoS ONE

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We report on the genomic characterization of 47 multi-drug resistant, carbapenem resistant and ESBL-producing K. pneumoniae isolates from the influent (I) and effluent (E) of three wastewater treatment plants (WWTPs) and from Romanian hospital units which are discharging the wastewater in the sampled WWTPs. The K. pneumoniae whole genome sequences were analyzed for antibiotic resistance genes (ARGs), virulence genes and sequence types (STs) in order to compare their distribution in C, I and E samples. Both clinical and environmental samples harbored prevalent and widely distributed ESBL genes, i.e. bla SHV , bla OXA , bla TEM and bla CTX M . The most prevalent carbapenemase genes were bla NDM-1 , bla OXA-48 and bla KPC-2 . They were found in all types of isolates, while bla OXA-162 , a rare bla OXA-48 variant, was found exclusively in water samples. A higher diversity of carbapenemases genes was seen in wastewater isolates. The aminoglycoside modifying enzymes (AME) genes found in all types of samples were aac(6'), ant(2'') Ia, aph(3'), aaD, aac(3) and aph(6). Quinolone resistance gene qnrS1 and the multi-drug resistance oqxA/B pump gene were found in all samples, while qnrD and qnrB were associated to aquatic isolates. The antiseptics resistance gene qacEdelta1 was found in all samples, while qacE was detected exclusively in the clinical ones. Trimethroprim-sulfamethoxazole (dfrA, sul1 and sul2), tetracyclines (tetA and tetD) and fosfomycin (fosA6, known to be located on a transpozon) resistance genes were found in all samples, while for choramphenicol and macrolides some ARGs were detected in all samples (catA1 and catB3 / mphA), while other (catA2,

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Section: Discussionmentioning

confidence: 99%

Whole genome sequencing snapshot of multi-drug resistant Klebsiella pneumoniae strains from hospitals and receiving wastewater treatment plants in Southern Romania

Surleac

Barbu

Paraschiv³

et al. 2020

PLoS ONE

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“…The minimum inhibitory concentration (MIC) values were investigated. 3 Bacteria exposed to sodium hypochlorite showed high tolerance comparing with other disinfectant used in this study. K.pneumoniae were the highest resistance bacteria as MBC result were 33.3 mg/ml.…”

Section: Determination Of the Minimum Inhibitory Concentrations And Mmentioning

confidence: 68%

“…Selection of disinfectant and antiseptic counter to pathogenic microorganisms is become a problem due to extensive use to check and control the growth of microbes in living and non-living entities. The extensive application of disinfectant and antiseptic products has encouraged the assumption: promotion of resistance in microbes especially in cross resistance to antibiotics [3][4][5][6] . Currently, chemicals are available in the market in the form of disinfectants and antiseptics having ingredients like halogen compounds, alcohol, chlorohexidine, sodium hypochlorite, peroxides, phenols and quaternary ammonium compounds (QAC) such as benzalkonium chloride 7,8 .…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Pathogenic Bacteria Showing Resistance against Disinfectants

Johny¹,

Alkhuzaee²

2019

J Pure Appl Microbiol

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Citation: Bassam Oudh Al-johny and Abdulelah M. Alkhuzaee, Isolation and Identification of Pathogenic Bacteria Showing Resistance against Disinfectants, J Pure Appl Microbiol., 2019; 13(4):2065-2072. https://doi.org/10.22207/JPAM.13.4.18 AbstractSuccessive use and misuse of disinfectant against certain microbes may trigger resistance, causing the problem of ineffectiveness of disinfectant due to development of resistance strains. Ultimately, it is speculated to raise public health issues worldwide. Keeping in view the said problem an experiment was designed to test the efficacy of different disinfectants against some pathogenic bacteria isolated from selected restaurants and cafeterias kitchen. A total of 45 isolates were purified and identified. Amongst them, three isolates; Klebsiella pneumoniae, Macrococcus caseolyticus, Staphylococcus sciuri were selected for their pathogenicity while Terribacillus halophilus were selected for significant tolerance to high temperature and salt regimes. Different parameters including Minimum Inhibitory Concentrations (MIC) and Minimum bactericidal concentration (MBC) were determined for disinfectant; sodium hypochlorite, benzalkonium chloride and chloroxylenol. MIC values was: 2500µg/ml to 5000µg/ ml for sodium hypochlorite; 0.54µg/ml to 17.3µg/ml for Benzalkonium chloride; 18.7µg/ml to 150µg/ ml for chloroxylenol. MBC values was: 5000µg/ml to 33320µg/ml for sodium hypochlorite; 8.6µg/ ml to 138µg/ml for Benzalkonium chloride; 75µg/ml to 300µg/ml for chloroxylenol. Conclusively, K.pneumoniae showed high resistance to sodium hypochlorite and benzalkonium chloride compared to other bacteria used in this study. Among the studied, Staphylococcus sciuri were the most tolerant and resistance to chloroxylenol.

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“…Here, tolerance remains even after the removal of the disinfectant, and it can be transferred across generations [16]. Several bacterial adaptation and tolerance mechanisms are known to be involved, such as modulation of cell growth rate, biofilm formation, efflux pump activity, fatty-acid profile, and the cell size, cell membranes and cell wall [16][17][18][19]. However, in general, tolerance development to QACs is still not fully understood [13].…”

Section: Introductionmentioning

confidence: 99%

Transporters and Efflux Pumps Are the Main Mechanisms Involved in Staphylococcus epidermidis Adaptation and Tolerance to Didecyldimethylammonium Chloride

et al. 2020

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Staphylococcus epidermidis cleanroom strains are often exposed to sub-inhibitory concentrations of disinfectants, including didecyldimethylammonium chloride (DDAC). Consequently, they can adapt or even become tolerant to them. RNA-sequencing was used to investigate adaptation and tolerance mechanisms of S. epidermidis cleanroom strains (SE11, SE18), with S. epidermidis SE11Ad adapted and S. epidermidis SE18To tolerant to DDAC. Adaptation to DDAC was identified with up-regulation of genes mainly involved in transport (thioredoxin reductase [pstS], the arsenic efflux pump [gene ID, SE0334], sugar phosphate antiporter [uhpT]), while down-regulation was seen for the Agr system (agrA, arC, agrD, psm, SE1543), for enhanced biofilm formation. Tolerance to DDAC revealed the up-regulation of genes associated with transporters (L-cysteine transport [tcyB]; uracil permease [SE0875]; multidrug transporter [lmrP]; arsenic efflux pump [arsB]); the down-regulation of genes involved in amino-acid biosynthesis (lysine [dapE]; histidine [hisA]; methionine [metC]), and an enzyme involved in peptidoglycan, and therefore cell wall modifications (alanine racemase [SE1079]). We show for the first time the differentially expressed genes in DDAC-adapted and DDAC-tolerant S. epidermidis strains, which highlight the complexity of the responses through the involvement of different mechanisms.

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Effects of exposure to quaternary-ammonium-based biocides on antimicrobial susceptibility and tolerance to physical stresses in bacteria from organic foods

Cited by 76 publications

References 71 publications

Whole genome sequencing snapshot of multi-drug resistant Klebsiella pneumoniae strains from hospitals and receiving wastewater treatment plants in Southern Romania

Whole genome sequencing snapshot of multi-drug resistant Klebsiella pneumoniae strains from hospitals and receiving wastewater treatment plants in Southern Romania

Isolation and Identification of Pathogenic Bacteria Showing Resistance against Disinfectants

Transporters and Efflux Pumps Are the Main Mechanisms Involved in Staphylococcus epidermidis Adaptation and Tolerance to Didecyldimethylammonium Chloride

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