Effects of Exposure to New Inhalational Anesthetics on Spermatogenesis and Sperm Morphology in Rabbits

Abstract

& The effects of chronic exposure to new inhalation anesthetics (sevoflurane and isoflurane) on spermatogenesis and sperm morphology were examined in 23 rabbits, randomly divided in 3 groups. Rabbits received 20 exposure hours (four hours=day  5 days), as follows: group I: 2.3% (1.2 MAC) sevoflurane þ 2L=min oxygen, group II: 1.3% (1.2 MAC) isoflurane þ 2L=min oxygen, and group III (control): 2L=min oxygen. Semen was collected on the 12th, 19th, 26th, 33rd, and 41st days of exposure. Sperm concentration, motility and morphological changes were evaluated. On the 41st day, testicular biopsies were taken and observed with light microscopy. Sperm concentration and motility significantly decreased in the sevoflurane and the isoflurane groups, compared to control. There were no significant changes in the control group. It is concluded that chronic exposure to the new inhalational anesthetics had negative effects on spermatogenesis and sperm morphology.

Unique features of spermiogenesis in the Musky Rat‐kangaroo: reflection of a basal lineage or a distinct fertilization process?

“…However, testicular tissue taken between 9 and 17 days after administration of isofluorane anaesthetic and testicular injection, had disruptions to the seminiferous cycle within varying cellular cohorts depending on time since injection. Inhalation anaesthetics in rabbits disrupts spermatogenesis (Ceyhan et al 2005) and this may also be true for the Musky Rat-kangaroo.…”

Personnel breathing zone sevoflurane concentration adherence to occupational exposure limits in conjunction with filling of vaporisers

“…T he potential negative health effects from work place exposure to trace concentrations of inhaled anaesthetics have been discussed since the 1960s 1 . Animal experimental data have linked chronic exposure to clinical doses of sevoflurane to extensive changes in spermatogenesis and sperm morphology 2 . Chronic exposure to trace concentrations is associated with both cognitive and haematological effects 3,4 .…”

The oxidative/anti-oxidative effects of sevoflurane on reproductive system of females: An experimental study

“…For sevoflurane exposure, rats were moved to a 40x50x60 cm anesthesia chamber, which had a connection with an anesthesia system (Prima SP Alpha, Penlon Limited, Oxon, UK). As previously described by Ceyhan et al ( 11 ) , two holes, one at the top left side of the chamber and the other at the upper right side of the chamber, were opened for anesthetic gas inlet and outlet. Animals were randomly separated into six groups, each group included five rats: the control group (C) was administered 2 L/min O 2 18 min/day for seven days; the first group (S1) was administered 1 minimum alveolar concentration (MAC) of sevoflurane and 2 L/min O 2 18 min/day for seven days; the second group (S2) was administered 1 MAC of sevoflurane and 2 L/min O 2 18 min/day for seven days with no treatment for the next seven days; the third group (S3) was administered 1 MAC of sevoflurane and 2 L/min O 2 18 min/day for 14 days; the fourth group (S4) was administered 1 MAC of sevoflurane and 2 L/min O 2 in 18 min/day for 14 days with no treatment for the next seven days; and the fifth group (S5) was administered 1 MAC of sevoflurane and 2 L/min O 2 18 min/day for 14 days with no treatment for the next 14 days.…”