Effects of explant type, culture media and growth regulators on callus induction and plant regeneration of Chinese jiaotou (Allium chinense)

Yan, Miaomiao; Xu, Chan; Kim, Chun-Hwan; Um, Yeongcheol; Bah, Amadou Apho; Guo, Danlu

doi:10.1016/j.scienta.2009.07.021

Cited by 41 publications

(16 citation statements)

References 32 publications

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“…Irrespective of the explants, 2,4-D was recommended to be the most widely used growth regulator for wheat callus induction and maintenance (Pellegrineschi et al, 2004). Yan et al (2009) reported that the best callus proliferation rate was observed on B5 medium supplemented with 1.5 mg L −1 2,4-D in the basal plate explants of Chinese jiaotou (Allium chinense). Our study revealed that a low concentration was inadequate for the optimal callus proliferation, whilst the concentration at 2.5 mg L −1…”

Section: Discussionmentioning

confidence: 99%

Effect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (<i>centella asiatica</i> L. urban)

Tan¹,

Musa²,

Ariff³

et al. 2010

American Journal of Biochemistry and Biotechnology

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Problem statement: Considering pegaga medicinal properties and over-exploitation, the requirement for a tissue culture technique as an alternative production system was crucial. Approach: Investigation of cell suspension culture response to different plant growth regulators (PRGs) for flavonoid production from elite cell line was carried out. Callus cultures were initiated from the leaf explants of Centella asiatica on Murashige and Skoog (MS) medium containing B5 vitamins and 30 g L −1 sucrose supplemented with different concentrations (0.5-2.5 mg L ). This combination was also found to be best for callus proliferation for all the accessions investigated. Among the four accessions tested, UPM03 was found to have the highest biomass yield (0.041 g DW culture

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Section: Discussionmentioning

confidence: 99%

Effect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (<i>centella asiatica</i> L. urban)

Tan¹,

Musa²,

Ariff³

et al. 2010

American Journal of Biochemistry and Biotechnology

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“…Several factors can influence shoot multiplication [16] such as selection of explant, media type [37], concentration of plant growth regulators, and carbon energy source [38] which maintains osmotic potential [39]. Carbon source is an essential supplement of tissue culture medium that regulates photosynthetic activity of explants.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Regeneration Potential of White Lupin (Lupinus albus) from Cotyledonary Nodes

Aslam

Karanja

Zhang

et al. 2020

Plants

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The tissue culture regeneration system of Lupinus albus has always been considered as recalcitrant material due to its genotype-dependent response and low regeneration efficiency that hamper the use of genetic engineering. Establishment of repeatable plant regeneration protocol is a prerequisite tool for successful application of genetic engineering. This aim of this study was to develop standardized, efficient protocol for successful shoot induction from cotyledonary node of white lupin. In this study, 5 day old aseptically cultured seedlings were used to prepare three explants (half cotyledonary node, HCN; whole cotyledonary node, WCN; and traditional cotyledonary node, TCN), cultured on four concentrations of M519 medium (M519, ½ M519, 1/3 M519, and ¼ M519), containing four carbohydrate sources (sucrose, fructose, maltose, and glucose), and stimulated with various combinations of KT (kinetin), and NAA (naphthalene acetic acid) for direct shoot regeneration. High frequency of 80% shoot regeneration was obtained on ½ M519 medium (KT 4.0 mg L−1 + NAA 0.1 mg L−1) by using HCN as an explant. Interestingly, combinations of (KT 4.0 mg L−1 + NAA 0.1 mg L−1 + BAP 1.67 mg L−1), and (KT 2.0 mg L−1 + NAA 0.1 mg L−1) showed similar shoot regeneration frequency of 60%. Augmentation of 0.25 g L−1 activated charcoal (AC) not only reduced browning effect but also improved shoot elongation. Among the all carbohydrate sources, sucrose showed the highest regeneration frequency with HCN. Additionally, 80% rooting frequency was recorded on ½ M519 containing IAA 1.0 mg L−1 + KT 0.1 mg L−1 (indole acetic acid) after 28 days of culturing. The present study describes establishment of an efficient and successful protocol for direct plant regeneration of white lupin from different cotyledonary nodes.

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“…Therefore, it takes many years to produce a sufficient number of seed bulbs for the practical cultivation of a new variety. Thus, in-vitro tissue culture techniques are feasible alternatives for improving the propagation efficiency, eliminating viruses from infected plants and breeding 2 .…”

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confidence: 99%

Effects of genotypes and explants on garlic callus production and endogenous hormones

Mostafa

Wang

Song

et al. 2020

Sci Rep

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High callus production is a feasible way to improve the propagation coefficient of garlic. It remains unknown how genotypes and explants affect garlic callus formation. In the present investigation, we found that there were significant differences in callus formation among garlic varieties. Tip explants were the best calli-producing source, and 91.05% of the explants from four varieties, on average, formed calli after 45 d of primary culturing. Upper leaf parts explants produced lower values. Among the different varieties and explant types, tip explants of variety T141 induced calli in the shortest time and had the greatest callus fresh weight at 45 d. An endogenous hormone contents analysis showed that auxins (indole-3-acetic acid and methyl indole-3-acetic acetate), cytokinins (trans-zeatin and dihydrozeatin), gibberellins 4, 9,15,19,24 and 53 , abscisic acid, jasmonic acid, jasmonoyl-L-isoleucine, and dihydrojasmonic acid were significantly greater in the tips than those in the upper leaf parts. High endogenous jasmonic acid content might play important roles in callus formation. These results will help us not only establish an efficient garlic callus induction protocol that can be applied to large-scale callus multiplication and regeneration, and to genetically improvement of garlic production, but also understand endogenous hormone roles in tissue/organ differentiation and dedifferentiation.

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Effects of explant type, culture media and growth regulators on callus induction and plant regeneration of Chinese jiaotou (Allium chinense)

Cited by 41 publications

References 32 publications

Effect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (<i>centella asiatica</i> L. urban)

Effect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (<i>centella asiatica</i> L. urban)

In Vitro Regeneration Potential of White Lupin (Lupinus albus) from Cotyledonary Nodes

Effects of genotypes and explants on garlic callus production and endogenous hormones

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