Effects of exopolysaccharide fractions with different molecular weights and compositions on fecal microflora during in vitro fermentation

Mao, Yu-Heng; Song, Ang Xin; Li, Long‐Qing; Siu, Ka Chai; Yao, Zhongping; Wu, Jianyong

doi:10.1016/j.ijbiomac.2019.12.072

Cited by 29 publications

(25 citation statements)

References 47 publications

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“…Preparation of fecal slurry was performed by using a previously described method with slight modifications [18]. The fresh fecal samples were provided by three healthy volunteers (ages 20-28) who had no history of gastrointestinal disease and were not treated with antibiotics within six months.…”

Section: Fecal Microbiota Regulatory Activity Of Eps 2101 Fecal Slurry Preparationmentioning

confidence: 99%

“…The effect of the EPS produced by Lpb. plantarum NMGL2 on the fecal microbiota change could be due to the relatively low molecular weight (3.03 × 10 4 Da) and monosaccharide composition of the EPS, which benefited digestion of the polymer by the microbiota and growth of certain microbes, e.g., Firmicutes in the early fermentation stage [18]. The EPS-induced increase in the abundance of Actinobacteria containing probiotic Bifidobacterium would be beneficial to human health.…”

Section: Otus Analysismentioning

confidence: 99%

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Characterization and In Vitro Fecal Microbiota Regulatory Activity of a Low-Molecular-Weight Exopolysaccharide Produced by Lactiplantibacillus plantarum NMGL2

Yao

Zhang

Lai

et al. 2022

Foods

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The exopolysaccharide (EPS) produced by Lactiplantibacillus plantarum NMGL2 isolated from traditional fermented dairy cheese was purified chromatographically with DEAE-Sepharose and Sepharose CL-6B columns. The purified EPS was characterized by various physicochemical methods and in vitro fecal microbiota regulation assay. The results showed that the EPS had a relatively low molecular weight of 3.03 × 104 Da, and it had a relatively high degradation temperature of 245 °C as determined by differential scanning calorimetry. Observation of the EPS by scanning electron microscopy, transmission electron microscopy, and atomic force microscopy revealed a highly branched and tangled fibrous network microstructure with many hollow microtubules and spherical particles. Structural study by 1H NMR spectroscopy suggested that the EPS contained a tetrasaccharide repeating unit with monosaccharide components of β-galactose (4.6%), α-glucose (20.6%), and α-mannose (74.8%). The EPS was highly resistant to hydrolysis of simulated human saliva, gastric, and intestinal juices. Moreover, the EPS beneficially affected the composition and diversity of the fecal microbiota, e.g., increasing the relative abundance of Firmicutes and inhibiting that of Proteobacteria. The results of this study indicated significant bioactivity of this novel low-molecular-weight EPS produced by Lpb. plantarum NMGL2, which could serve as a bioactive agent for potential applications in the food and health care industry.

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Section: Fecal Microbiota Regulatory Activity Of Eps 2101 Fecal Slurry Preparationmentioning

confidence: 99%

Section: Otus Analysismentioning

confidence: 99%

Characterization and In Vitro Fecal Microbiota Regulatory Activity of a Low-Molecular-Weight Exopolysaccharide Produced by Lactiplantibacillus plantarum NMGL2

Yao

Zhang

Lai

et al. 2022

Foods

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“…Полимеры обеспечивают посредничество в межбактериальных взаимодействиях или/и изменяют колонизационные и персистентные свойства бифидофлоры в различных экологических нишах [22,23]. ЭПС могут выступать в качестве микроб-ассоциированных молекулярных паттернов и взаимодействовать с клетками макроорганизма, способствуя формированию биопленок [12,14] [24,25]. В целом предполагают, что ЭПС у бифидобактерий выполняют те же функции, что и у других микроорганизмов.…”

Section: биологическая роль эпс бифидобактерийunclassified

“…Относительно недавно Salazar et al сообщили о способности кишечной микробиоты использовать ЭПС лактобактерий и бифидобактерий в качестве субстратов для питания [28]. На моделях in vitro показана способность ЭПС бифидобактерий изменять профиль и разнообразие кишечной микробиоты, вызывать сдвиги в метаболической активности микросимбионтов, в частности в синтезе короткоцепочечных жирных кислот [25]. Способность B. fragilis вызывать деградацию ЭПС бифидобактерий была доказана путем их культивирования на минимальной среде с полимерами бифидобактерий в качестве единственного источника углерода.…”

Section: биологическая роль эпс бифидобактерийunclassified

Bifdobacterial exopolysaccharides: a brief review

Захарова¹,

Леванова²,

Отдушкина³

2021

Fundamentalʹnaâ i kliničeskaâ medicina

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Exopolysaccharide (EPS) production is a widespread phenotypic trait in many commensal and pathogenic microorganisms. In bifidobacteria, the discovery of the eps gene cluster propelled the multiple studies of their EPS, which represent heteropolysaccharides and generally consist of three monosaccharides: D-glucose, D-galactose, and L-rhamnose. EPS of B. animalis subsp. lactis additionally contains mannose while EPS of B. adolescentis and B. longum subsp. longum contains 6-deoxytealose. The number of repeat units in bifidobacterial EPS is a straincharacteristic feature. Precursors of the indicated EPS monomers are glucose-1-phosphate and fructose-6-phosphate, and the synthesis involves nucleotide sugar intermediates. Two molecular systems are implica in polymerisation and polymer secretion in bifidobacteria: ABC transporters and fippase polymerase complex (Wzx/Wzydependent pathway). EPS perform numerous functions. They protect bifidobacteria from aggressive gastrointestinal milieu and reactive oxygen species, provide a scaffold for the bacterial-bacterial interactions, and act as the receptors for phage adsorption. Further, EPS are used by the other members of the gut microbiota as substrates for nutrition, i.e. bifidobacteria regulate the composition and metabolic activity of intestinal microorganisms. Therefore, EPS-producing strains exhibit pronounced antibacterial effects due to the binding of opportunistic and pathogenic microbes. Finally, EPS can act as pathogen-associated molecular patterns. Beneficial effects of bifidobacterial EPS determined the possibility of their use as prebiotics or as a part of symbiotics. The main limitation in this regard is the low yield of the target product when culturing EPS-producing strains. Therefore, current research is aimed at finding novel EPSproducing strains among the bifidobacteria and creating favorable technological conditions that promote EPS production.

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“…Non-cultural methods of bacteria enumeration, such as fluorescence in situ hybridization (FISH) and polymerase chain reaction (PCR), have greatly enhanced the accuracy of prebiotic activity assessment. The range of analyzed prebiotics has been greatly expanded recently: exopolysaccharide from Cordyceps sinensis Cs-HK1 [20], xylooligosaccharides produced by Bacillus subtilis 3610 [21], extracts of brown seaweed [22], and so on. Basing on the data obtained from the fermentation of fecal cultures, Palframan, Gibson, and Rastall [23] suggested calculation of a prebiotic index (PI), which was determined as the difference between the number of probiotics and non-probiotics at the end of the fermentation.…”

Section: Introductionmentioning

confidence: 99%

Online Database for Retrieval Information about Prebiotics and Their Activity

et al. 2020

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The number of studies aimed at proving the prebiotic properties of certain substances or compositions has been actively increasing, which has led to a large accumulation of scientific information that is fragmented and not systematized. Moreover, a number of criteria have been applied in these studies. The lack of an accessible and convenient information space to compare the obtained results seems to hold back not only scientific development, but also practical development in this field. A database called the «On-line Database of Researches on Activity of Prebiotics» (ODRAP) is presented in this article, which contains information about both prebiotics and some probiotics, that were used in these researches. Currently, ODRAP collects 25 bacteria genera or their combinations, 59 bacteria species, 140 prebiotic substances, 61 prebiotic production companies, 2 methods of fermentation, and 271 analyzed articles from 2001 till 2019. To facilitate access to the database, a special Web-interface was created, which allows any user who opens the Web-page to obtain information about the features and activities of prebiotics, as well as to sort the data by species and genus of bacteria applied in tests, the chemical nature or source of prebiotics, and other parameters. The convenience of the Web-interface is that it allows access to the database, regardless of the user platform and from anywhere, via the Internet.

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Effects of exopolysaccharide fractions with different molecular weights and compositions on fecal microflora during in vitro fermentation

Cited by 29 publications

References 47 publications

Characterization and In Vitro Fecal Microbiota Regulatory Activity of a Low-Molecular-Weight Exopolysaccharide Produced by Lactiplantibacillus plantarum NMGL2

Characterization and In Vitro Fecal Microbiota Regulatory Activity of a Low-Molecular-Weight Exopolysaccharide Produced by Lactiplantibacillus plantarum NMGL2

Bifdobacterial exopolysaccharides: a brief review

Online Database for Retrieval Information about Prebiotics and Their Activity

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