Effects of exogenous salicylic acid on polysaccharides production of Dendrobium officinale

Abstract

Polysaccharides from Dendrobium officinale possess unparalleled medicinal value. In order to produce the active polysaccharides from D. officinale through tissue culture, the effects of salicylic acid on the accumulation of polysaccharides were investigated. Salicylic acid (SA) was beneficial to increasing the contents of polysaccharides. The highest polysaccharide production occurred on the medium supplied with 100 μmol·L −1 salicylic acid. After 30 days of culture the production of polysaccharides reached 10.09% and 15.81% by phenol-sulfuric acid method and 3, 5-dinitrosalicylic (DNS) colorimetric method respectively. The major constituent of polysaccharides, glucose and mannose, was determined by pre-column derivatization-HPLC. The results showed that the glucose contents were reduced and mannose contents were increased with different salicylic acid concentration. The ANOVA of the mannose contents demonstrated that among the different SAs concentration was significantly different. The SA application influenced polysaccharide production rather than degradation because the sucrose metabolic enzyme activities were modified by SA. The experiments suggest that salicylic acid could be an effective compound to enhance the production of active polysaccharides from D. officinale.

Salicylic acid-induced abiotic stress tolerance and underlying mechanisms in plants

“…SA was reported to inhibit valine and sucrose uptake in a concentration-dependent manner (10–200 μM; Bourbouloux et al, 1998 ). Improved plant health can also be achieved with increased contents of polysaccharides and soluble sugars, respectively, with 100 μmol L -1 ( Yuan et al, 2014 ), and 0.5 and 1.0 mM SA ( Luo et al, 2014 ).…”

Preparation and characterization of Dendrobium officinale powders through superfine grinding

“…Dendrobium officinale , called ‘Tie‐Pi‐Shi‐Hu’ in Chinese, is a perennial evergreen herbaceous plant distributed mainly in the southern area of China, especially in southern Anhui, eastern Zhejiang, western Fujian, north‐western Guangxi, Sichuan and south‐eastern Yunnan provinces . According to Traditional Chinese Medicine, D. officinale has outstanding medicinal qualities, such as immunomodulatory, antineoplastic, antioxidative and antimutagenic activities . From ancient times onward, D. officinale has long been used as a therapeutic agent for nourishing the stomach, promoting saliva secretion and reducing fever, in addition to being used as an ingredient in nutraceutical beverages and food products .…”

Changes in Morphological Characteristics, Regeneration Ability, and Polysaccharide Content in Tetraploid Dendrobium officinale

“…They are a source of tonic, astringent, analgesic, antipyretic, and anti-inflammatory substances (Cakova et al, 2017). Regarded as one of the most famous Dendrobium in China since the Tang dynasty 1300 years ago, D. officinale Kimura et Migom has been used to eliminate heat evil, remediate stomach problems, moisten the lung, relieve cough, nourish the body, and prolong lifespan (Alonso-Sande et al, 2009;Wang et al, 2018;Yuan et al, 2014). More than 190 compounds have been isolated from D. officinale (Tang et al, 2017), of which polysaccharide is one of the major active constituents, exhibiting immunomodulatory and cardioprotective activities (Dou et al, 2016;He et al, 2016).…”