Effects of exogenous nitric oxide on antioxidation and DNA methylation of Dendrobium huoshanense grown under drought stress

Fan, Honghong; Li, Tingchun; Guan, Lei; Li, Zhengpeng; Guo, Na; Cai, Yongping; Lin, Yi

doi:10.1007/s11240-011-0096-3

Cited by 45 publications

(26 citation statements)

References 25 publications

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“…It was demonstrated that drought stress had a great impact on genomic methylation alterations in perennial ryegrass. These results are similar to those in previous reports on drought-treated rice and Dendrobium huoshanense (Fan et al 2012). In addition, our findings showed that fully methylated DNA levels dropped significantly, by 12.58 %, although the number of hemi-methylated sites increased slightly after drought stress.…”

Section: Discussionsupporting

confidence: 92%

Analysis of DNA methylation of perennial ryegrass under drought using the methylation-sensitive amplification polymorphism (MSAP) technique

et al. 2014

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in two other genes decreased, the sites of methylation remained, and the expression increased only slightly. All of these results suggested that drought stress decreased the total DNA methylation level in perennial ryegrass and demethylation up-regulated related gene expressions and that the extent of methylation was negatively correlated with expression. Overall, the induced epigenetic changes in genome probably are an important regulatory mechanism for acclimating perennial ryegrass to drought and possibly other environmental stresses.

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Section: Discussionsupporting

confidence: 92%

Analysis of DNA methylation of perennial ryegrass under drought using the methylation-sensitive amplification polymorphism (MSAP) technique

et al. 2014

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“…Although accumulation of NO during drought stress appears to be a general response in diverse plant species and tissues, its specificity has only been established by using various inhibitors / scavengers such as c-PTIO [2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide] or L-NAME [(N(G)-nitro-L-arginine-methyl ester] that reverse the process(s) [7,14,17,18,23,25,30,38,39]. Similarly, the kinetics of NO release from donors depends on many factors such as the reactivity of donor, surrounding environment, chemical nature of tissue, light, concentration and active duration of exposure etc.…”

Section: Exogenous No Donors In Drought Stress Ameliorationmentioning

confidence: 99%

“…While drought stress decrease the DNA methylation levels in Dendrobium huoshanense, NO increased the demethylation ratio of methylated sites indicating that NO can trigger gene expression under drought [39]. Many studies used high throughput technologies to identify the NO-responsive genes following exogenous application of NO donors or inhibitors [52,77,78].…”

Section: No-responsive Genes and Functional Validation Studiesmentioning

confidence: 99%

NO to drought-multifunctional role of nitric oxide in plant drought: Do we have all the answers?

2015

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Nitric oxide (NO) is a versatile gaseous signaling molecule with increasing significance in plant research due to its association with various stress responses. Although, improved drought tolerance by NO is associated greatly with its ability to reduce stomatal opening and oxidative stress, it can immensely influence other physiological processes such as photosynthesis, proline accumulation and seed germination under water deficit. NO as a free radical can directly alter proteins, enzyme activities, gene transcription, and post-translational modifications that benefit functional recovery from drought. The present drought-mitigating strategies have focused on exogenous application of NO donors for exploring the associated physiological and molecular events, transgenic and mutant studies, but are inadequate. Considering the biphasic effects of NO, a cautious deployment is necessary along with a systematic approach for deciphering positively regulated responses to avoid any cytotoxic effects. Identification of NO target molecules and in-depth analysis of its effects under realistic field drought conditions should be an upmost priority. This detailed synthesis on the role of NO offers new insights on its functions, signaling, regulation, interactions and co-existence with different drought-related events providing future directions for exploiting this molecule towards improving drought tolerance in crop plants.

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“…Class I: non-methylated fragments, Class II: hemi-methylated fragments; Classes III and IV: fully methylated fragments R. glutinosa to lead to a pronounced decline in the yield of tuberous roots , an effect which was reproduced in the present experiment. The replanting disease syndrome is thought to reflect the modulation of gene expression (Richards 1997;Ding et al, 2014;Yang et al, 2015;Naydenov et al, 2015), most likely arising from epigenetic mechanisms (Fan et al, 2012;Ding et al, 2014). The present MSAP profiling showed that the global level of methylation level was somewhat (about 6.58%) higher in the R2 than in the R1 material.…”

Section: Discussionmentioning

confidence: 57%

DNA Methylation in Rehmannia glutinosa Roots Suffering from Replanting Disease

Yang¹,

Li²

2015

IJAB

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Abstract"Replanting disease" is a serious constraint to root growth in the medicinal species Rehmannia glutinosa (Gaertn.) Libosch. ex Fisch. and C.A. Mey. The syndrome involves an array of morphological, physiological and biochemical changes to the plant, which culminates in a major loss in tuberous root growth. Here, the tendency of replanting disease to induce differential cytosine methylation in the root DNA was explored via the methylation-sensitive amplified polymorphism (MSAP) method. Exposure to the disease measurably altered the global methylation level. Of the 231 differentially methylated MSAP fragments identified, 136 involved replanting disease-induced methylation and 95 demethylation. A set of 31 differentially methylated fragments was isolated and sequenced. The sequences were used to analyze the function of the genes involved and to investigate whether any were differentially transcribed as a result of exposure to replanting disease. Of the eight genes subjected to transcription profiling, the three which were demethylated in the diseased roots were transcribed more abundantly in these roots, and the five which were methylated were down-regulated by a real-time quantitative PCR (qPCR) method. Our study gives an insight into the DNA methylation of R. glutinosa subjected to replanting disease and provides valuable information for further exploring epigenetic regulation of responses to the disease in the species and other plants.

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Effects of exogenous nitric oxide on antioxidation and DNA methylation of Dendrobium huoshanense grown under drought stress

Cited by 45 publications

References 25 publications

Analysis of DNA methylation of perennial ryegrass under drought using the methylation-sensitive amplification polymorphism (MSAP) technique

Analysis of DNA methylation of perennial ryegrass under drought using the methylation-sensitive amplification polymorphism (MSAP) technique

NO to drought-multifunctional role of nitric oxide in plant drought: Do we have all the answers?

DNA Methylation in Rehmannia glutinosa Roots Suffering from Replanting Disease

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