Effects of exogenous fibrolytic enzymes on ruminal fermentation and digestion of Guinea grass hay

Avellaneda, J.; Pinos-Rodríguez, Juan Manuel; González, S.; Bárcena, R.; Hernández, A.; Cobos, M.A.; Hernández, D.; Montañez, O. D.

doi:10.1016/j.anifeedsci.2008.05.003

Cited by 21 publications

(13 citation statements)

References 21 publications

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“…The results from this Rusitec study are in agreement with previous in situ work carried out with bermudagrass (C. dactylon) silages (Mandebvu et al, 1999) and with bermudagrass hay and Pensacola bahiagrass (P. notatum) hay (Dean et al, 2008) and in vivo with Guinea grass (P. maximum var. Mombasa) hay (Avellaneda et al, 2009), studies which did not find any effects of fibrolytic enzymes on the digestion of these high-fibre tropical grasses.…”

Section: Rusitecmentioning

confidence: 90%

“…Some studies have examined the application of enzymes to tropical forages, with little benefit to the feed value of bermudagrass (Cynodon dactylon) silages (Mandebvu et al, 1999), bermudagrass, Pensacola bahiagrass (Paspalum notatum) (Dean et al, 2008) or Guinea grass (Panicum maximum var. Mombasa; Avellaneda et al, 2009). The large variation in nutritional quality among tropical grasses, along with differences in preservation method (fresh forage, hay and silage), suggest that there is a need to further screen fibrolytic enzyme additives for their ability to improve the digestibility of tropical forages.…”

Section: Introductionmentioning

confidence: 99%

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Effect of fibrolytic enzymes added to a Andropogon gayanus grass silage-concentrate diet on rumen fermentation in batch cultures and the artificial rumen (Rusitec)

Ribeiro

Gonçalves

Pereira

et al. 2015

Animal

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In vitro batch cultures were used to screen four fibrolytic enzyme mixtures at two dosages added to a 60 : 40 silage : concentrate diet containing the C 4 tropical grass Andropogon gayanus grass ensiled at two maturities -vegetative stage (VS) and flowering stage (FS). Based on these studies, one enzyme mixture was selected to treat the same diets and evaluate its impact on fermentation using an artificial rumen (Rusitec). In vitro batch cultures were conducted as a completely randomized design with two runs, four replicates per run and 12 treatments in a factorial arrangement (four enzyme mixtures × three doses). Enzyme additives (E1, E2, E3 and E4) were commercial products and contained a range of endoglucanase, exoglucanase and xylanase activities. Enzymes were added to the complete diet 2 h before incubation at 0, 2 and 4 μl/g of dry matter (DM). Gas production (GP) was measured after 3, 6, 12, 24 and 48 h of incubation. Disappearance of DM (DMD), NDF (NDFD) and ADF (ADFD) were determined after 24 and 48 h. For all four enzyme mixtures, a dosage effect (P < 0.05) was observed for NDFD and ADFD after 24 h and for DMD, NDFD and ADFD after 48 h of incubation of the VS diet. For the FS diet, a dosage effect was observed for GP and NDFD after 24 h and for GP, DMD, NDFD and ADFD after 48 h of incubation. There was no difference among enzyme mixtures nor was there an enzyme × dose interaction for the studied parameters. Because of the greatest numerical effect on NDF disappearance and the least cost price, enzyme mixture E2 at 4 µl/g of diet DM was selected for the Rusitec experiment. The enzyme did not impact (P > 0.05) DM, N, NDF or ADF disappearance after 48 h of incubation nor daily ammonia-N, volatile fatty acids or CH 4 production. However, enzyme application increased (P < 0.05) microbial N production in feed particle-associated (loosely-associated) and silage feed particle-bound (firmly associated) fractions. With A. gayanus silage diets, degradation may not be limited by microbial colonization, but rather by the ability of fibrolytic enzymes to degrade plant cell walls within this recalcitrant forage.

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Section: Rusitecmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Effect of fibrolytic enzymes added to a Andropogon gayanus grass silage-concentrate diet on rumen fermentation in batch cultures and the artificial rumen (Rusitec)

Ribeiro

Gonçalves

Pereira

et al. 2015

Animal

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“…Enhanced TDN, CP digestibility with normal blood and rumen parameters in sheep were observed by Gomaa et al (2012) after treating rations with exogenous enzymes. As suggested by the study of Avellaneda et al (2009) fibrolytic enzymes reduced duodenal ADF flow and forestomach digestion and there were no enzyme effects on DMI, N balance, ruminal degradation, total tract digestion, ruminal fermentation, as well as ruminal protozoal counts. Mota et al (2011) conducted a study to evaluate the effect of exogenous enzyme, glucoamylase from Aspergillus niger addition in finishing diets for lambs.…”

Section: Sheepmentioning

confidence: 99%

Exogenous Enzymes in Ruminant Nutrition: A Review

Sujani¹,

Seresinhe²

2015

Asian J. of Animal Sciences

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The concept of supplementation of ruminant diets with exogenous enzymes is not new though a considerable research interest on this field has been emerged from 1990s. Availability of wide range of exogenous enzyme products, development of better methods to evaluate enzyme activity, revised knowledge on rumen functions and recent advances of biotechnology which lowers the cost of enzyme production have fuelled the enzyme utilization in ruminant industries. The exogenous enzymes used in ruminant diets can be characterized in to main categories as fibrolytic, amylolytic and proteolytic based on specific substrate on which their enzyme activity can perform. The sources of these exogenous enzymes are mainly four bacterial species, three fungal species and some yeasts. Solid State Fermentation (SSF) and Submerged Fermentation (SmF) are the major methods for enzyme extraction which have combined with several other biotechnological aspects. The enzyme application method can vary in a wide range from applying to forage at harvesting, at ensiling, at feeding and the portion of feed which is mixed with enzyme may also vary from to forage to concentrate to a part of totally mixed rations. Up to date numerous number of researches have been done with ruminants like cattle both dairy and beef, goat, sheep and less with buffalo. The results seems to be inconsistent but positive results on feed intake, nutrient digestibility, growth performance, other production parameters, manure nutrient excretion etc., have been obtained. Therefore more fine-tuned research efforts are highly suggested for the generalization of exogenous enzyme usage in ruminant nutrition.

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“…Estas respuestas concuerdan a las reportadas por Yescas et al (2002) quienes evaluaron un complejo enzimático fibrolítico exógeno sobre la degradabilidad de dietas completas que contenían rastrojo de avena y maíz, para estos autores la degradabilidad in situ de la MS de dietas completas no fue diferente (p>0.05) a las 12, 48 y 72 h, sin embargo, a las 24 h fue mayor (p≤0.05) la degradabilidad de la dieta con paja de avena, sin ser lo último efecto del complejo fibrolítico exógeno. Similarmente, Moreno et al (2007) no encontraron efecto del complejo enzimático después de las 12 horas de incubación; así como, también Avellaneda et al (2009) en su estudio valorando el efecto de las enzimas en la digestión del pasto Panicum maximum. La mayor respuesta observada en la degradabilidad de la MS de pasto de 35 d, puede deberse al mayor contenido de carbohidratos de fácil fermentación y al menor contenido de compuesto fenólicos que limiten el acceso por parte de los microorganismos ruminales.…”

Section: Resultados Y Discusión Degradabilidad In Situ De La Ms Dietaunclassified

Enzimas fibrolíticas exógenas en la degradación ruminal in situ del pasto king grass (Pennisetum hybridum) en dos edades de corte

Zamora¹,

Álvarez²,

Moreno³

et al. 2016

CyT

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Effects of exogenous fibrolytic enzymes on ruminal fermentation and digestion of Guinea grass hay

Cited by 21 publications

References 21 publications

Effect of fibrolytic enzymes added to a Andropogon gayanus grass silage-concentrate diet on rumen fermentation in batch cultures and the artificial rumen (Rusitec)

Effect of fibrolytic enzymes added to a Andropogon gayanus grass silage-concentrate diet on rumen fermentation in batch cultures and the artificial rumen (Rusitec)

Exogenous Enzymes in Ruminant Nutrition: A Review

Enzimas fibrolíticas exógenas en la degradación ruminal in situ del pasto king grass (Pennisetum hybridum) en dos edades de corte

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