Effects of Ethanolic Extract of Raphanus Sativus Seeds on Fertility Hormone and Sperm Parameters in Male Wistar Rats

Abstract

This study was conducted to assess the effect of Raphanus sativus extract on the reproductive performance of male wistar rats. a total of 24 male rats were divided into 4 groups(6 of each) namely group A, B,C and D. All groups were given orally 100, 200, 400 and 0.0, mg/kg body weight extract respectively for 30 days. Symptoms caused by acute toxicity were absorbed through the experimental period. At the end of the experiment body weights and sexual organs weights (testes and epididyims) and semen characteristic were determined. Serum was analyzed for testosterone, Follicle-stimulating hormone (FSH), and Luteinizing hormone (LH) levels. The results showed the body weights was significantly (P≤0.01) decreased, while testicular weight increased. In a dose dependant manner following administration of R. sativus extracts. The extract also improved semen characteristics and elevated significantly the level of fertility hormones. it study conclude that the administration of the Raphanus sativus seeds extract improve normal male rats fertility.

Ameliorative Effect of Moringa oleifera Extract on Male Fertility in Paroxetine Treated Rats

“…Kujo [20] illustrated that the presence of flavonoids in Moringa oleifera extract have a role in altering androgen levels. In the current study, the improvement of reproductive hormones was in agreement with Dafaalla et al [21] who mentioned that Moringa oleifera extract improved the testosterone secretion and consequently its availability to gonads. The hormonal alterations induced by paroxetine administration recorded in this study (reduced the serum levels of FSH, LH, testosterone and estrogen) directly resulted in deterioration of the spermatogenesis, which was evidenced by marked reduction in sperm count and motility percentages, a significant increase of sperm abnormalities percentage and sperm DNA fragments.…”

Improving Libido and Sperm Quality of Bali Bulls by Supplementation of Moringa oleifera Leaves

“…The higher plasma concentrations of testosterone during the Moringa leaves supplementation was in accordance with the study in rats by Prabsattroo et al (2015) and Dafaalla et al (2016). They reported that Moringa leaves supplementation significantly increased serum testosterone in the rat.…”

“…They reported that Moringa leaves supplementation significantly increased serum testosterone in the rat. The treatment of Moringa leaves could also increase Leydig cells (Prabsattroo et al, 2015) and the levels of FSH and LH (Dafaalla et al, 2016). The testosterone synthesis performed by Leydig cells in the testes depends on the adequacy of Zn in the diet (Roy et al, 2013).…”

Androgenic effect of aqueous leaf extract of Moringa oleifera on Leydig TM3 cells in vitro

“…The primary source of androgens (mainly testosterone) in males is Leydig cells, which is essential for the regulation and differentiation of germ cells during spermatogenesis as well as in maintaining male reproductive function and health (Liu et al, 2014; Teerds & Huhtaniemi, 2015; Tremblay, 2015; Yuan, Ma, Ye, & Piao, 2016). MO has been shown to increase testosterone in animal models (Dafaalla et al., 2017; Ogunsola et al., 2017; Suarni, Oka, Mahardika, & Suyadnya, 2019; Syarifuddin, Toleng, Rahardja, Ismartoyo, & Yusuf, 2017). The focus of our study was to investigate the direct effect of aqueous leaf extract of MO on the Leydig cells.…”

“…Traditionally, MO is used to enhance male sexual functions such as libido, improve sperm quality and as an aphrodisiac in the treatment of sexual dysfunction (Lalas & Tsaknis, 2002; Prabsattroo, Wattanathorn, Iamsaard, Muchimapura, & Thukhammee, 2012). For instance, studies have shown increased weight of male reproductive organs, sperm parameters, luteinising hormone (LH), follicle‐stimulating hormone (FSH), testosterone levels, as well as enhanced sexual activities in rats (Dafaalla, Abass, Abdoun, Hassan, & Idris, 2017; Ogunsola, Joshua, Sunday, Nl, & As, 2017; Prabsattroo et al., 2012; Zade, Dabhadkar, Thakare, & Pare, 2013). In mice, however, an increase in the weight of reproductive organs, the diameter of the seminiferous tubule and thickness of the epididymal wall were observed, without affecting LH and FSH (Cajuday & Pocsidio, 2010).…”