Effects of Estradiol on the Paracrine Regulator Expression of In Vitro Maturated Murine Ovarian Follicles

Kim, Yong Jin; Park, Kyung Eui; Kim, Yoon Young; Kim, Hoon; Ku, Seung‐Yup; Suh, Chang Suk; Kim, Seok Hyun; Choi, Young Min

doi:10.1007/s13770-016-0006-1

Cited by 17 publications

(14 citation statements)

References 31 publications

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“…The methods used for in vitro culture of follicles have been taken from previous studies (Kim et al , , ,c). Non‐coated and alginate‐coated single and multiple follicles were placed in droplets (20–40 μl) of culture media on a culture dish (Falcon 353004, 60 × 15 mm; BD Biosciences, Irvine, CA, USA), one follicle or follicle cluster per droplet, 25 droplets per dish.…”

Section: Methodsmentioning

confidence: 99%

Induction of multiple ovulation via modulation of angiotensin II receptors in in vitro ovarian follicle culture models

Kim

Kang

et al. 2016

J Tissue Eng Regen Med

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In vitro culture of ovarian follicles is a promising bioengineering technique for retrieving fertilizable oocytes from preserved ovarian tissues of cancer survivors. However, current in vitro follicle culture techniques are labour-intensive and of low efficiency, as only single follicle culture (SFC) has been possible to date. The present study investigated the feasibility of multifollicular cluster culture (MFCC) system using angiotensin II receptor (ATII-Rc) analogues. Ovarian pre-antral follicles isolated from 2-week-old C57BL6 mice were cultured with ATII-Rc agonist or antagonist and their maturation outcomes were compared with control group. When single follicles were cultured, the ovulation and maturation rates were similar in all three groups. When three-follicle clusters were cultured, up to three follicles were ovulated in the ATII-Rc agonist group while none or one follicle ovulated in control or antagonist groups (p < 0.0001). Significantly higher numbers of mature oocytes were obtained in the agonist group (three-follicle 28.2 ± 4.9 vs. SFC 11.0 ± 1.3, per 25 cultured droplets) (p < 0.0001), and the development of each fertilized oocytes was comparable to those from SFC. It is therefore concluded that this novel MFCC system can significantly improve the efficiency of in vitro mature oocyte retrieval via ATII-Rc modulation. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Methodsmentioning

confidence: 99%

Induction of multiple ovulation via modulation of angiotensin II receptors in in vitro ovarian follicle culture models

Kim

Kang

et al. 2016

J Tissue Eng Regen Med

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“…The ovary offers a safe storage space for oocytes within follicles (Y. Y. Kim et al, ; Y. Y. Kim, J. W. Yun et al, ; Ross, ; Tamadon, Park, Kim, Kang, & Ku, ). It releases steroid hormones after puberty and thereby fosters follicular development and periodic oocyte release (Y. J. Kim et al, ; Y. J. Kim, Ku, Rosenwaks et al, ; Y. J. Kim, K. E. Park et al, ). Ideally and anatomically, the artificial ovary needs to contain follicles isolated from cryopreserved ovarian tissue and possibly other ovarian cells to provide growth factors (Chiti et al, ).…”

Section: Theoretical Overview Of the Artificial Ovarymentioning

confidence: 99%

“…Inflammatory responses can be evaluated through CD45 and F4/80 immunostaining (Laronda et al, ; Vanacker, Dolmans, Luyckx, Donnez, & Amorim, ). Ovarian functional recovery in mice can be validated with lowered serum follicle stimulating hormone concentration, elevated estrogen level, increased inhibin level, and pregnancy (Carroll & Gosden, ; Kim, Perez, et al, ; Kim, Park, et al, ; Y. Y. Kim, H. Kim, Ku et al, ; Laronda et al, ; Laronda et al, ; Smith et al, ).…”

Section: Evaluation and Monitoringmentioning

confidence: 99%

“…J. Kim, Ku, Rosenwaks et al, 2010; Y. J. Kim, K. E. Park et al, 2017). Ideally and anatomically, the artificial ovary needs to contain follicles isolated from cryopreserved ovarian tissue and possibly other ovarian cells to provide growth factors (Chiti et al, 2017).…”

Section: Theoretical Overview Of the Artificial Ovarymentioning

confidence: 99%

“…Ovarian functional recovery in mice can be validated with lowered serum follicle stimulating hormone concentration, elevated estrogen level, increased inhibin level, and pregnancy (Carroll & Gosden, 1993; Kim, Perez, et al, 2016;Kim, Park, et al, 2017;Laronda et al, 2015;Laronda et al, 2017;Smith et al, 2014).…”

Section: When Heterotopic Transplantation Is Conducted Several Factorsmentioning

confidence: 99%

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A new possibility in fertility preservation: The artificial ovary

Cho

Kim

Noh

et al. 2019

J Tissue Eng Regen Med

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Conventional fertility preservation methods such as oocyte or embryo cryopreservation are currently insufficient to treat including those patients with prepubertal cancer and premature ovarian failure. Ovarian tissue cryopreservation presents as an alternative but has limitations with a potential risk of reintroducing malignant cells in patients who recover from cancer, those of chemotherapy prior to tissue cryopreservation. The so called “artificial ovary” aims to resolve this issue by transplanting isolated follicles with or without a biological scaffold. The artificial ovary may also offer an effective alternative option for those who cannot benefit from traditional assisted reproductive techniques such as in vitro fertilisation. To date, in animal studies and human trial, the artificial ovary restored endocrine function, achieved in vivo follicular development, and resulted in successful pregnancies. However, development of a technique for higher follicular recovery rate and a more optimised design of delivery scaffold, better transplantation techniques to prevent postsurgical ischemia, and consideration for genetic safety are required for safer and consistent human clinical applications. Ideas from different transplantation surgeries (e.g., entire ovary, ovarian cortex, and transplantation with tissue‐engineered products) can be applied to enhance the efficacy of artificial ovarian transplantation. For the better application of artificial ovary, a deeper understanding of mechanical and biochemical properties of the ovary and folliculogenesis after cryopreservation, transplantation with or without scaffold, and development of sophisticated in vivo imaging techniques of transplanted artificial ovary need to precede its efficient clinical application.

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Preservation of fertility in female and male prepubertal patients diagnosed with cancer

Albamonte,

Vitullo

2023

J Assist Reprod Genet

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Over the past two decades, the importance of fertility preservation has grown not only in the realm of medical and clinical patient care, but also in the field of basic and applied research in human reproduction. With advancements in cancer treatments resulting in higher rates of patient survival, it is crucial to consider the quality of life post-cure. Therefore, fertility preservation must be taken into account prior to antitumor treatments, as it can significantly impact a patient’s future fertility. For postpubertal patients, gamete cryopreservation is the most commonly employed preservation strategy. However, for prepubertal patients, the situation is more intricate. Presently, ovarian tissue cryopreservation is the standard practice for prepubertal girls, but further scientific evidence is required in several aspects. Testicular tissue cryopreservation, on the other hand, is still experimental for prepubertal boys. The primary aim of this review is to address the strategies available for possible fertility preservation in prepubertal girls and boys, such as ovarian cryopreservation/transplantation, in vitro follicle culture and meiotic maturation, artificial ovary, transplantation of cryopreserved spermatogonia, and cryopreservation/grafting of immature testicular tissue and testicular organoids.

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Effects of Estradiol on the Paracrine Regulator Expression of In Vitro Maturated Murine Ovarian Follicles

Cited by 17 publications

References 31 publications

Induction of multiple ovulation via modulation of angiotensin II receptors in in vitro ovarian follicle culture models

Induction of multiple ovulation via modulation of angiotensin II receptors in in vitro ovarian follicle culture models

A new possibility in fertility preservation: The artificial ovary

Preservation of fertility in female and male prepubertal patients diagnosed with cancer

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