Effects of estradiol and progesterone on prolactin transcriptional activity in somatolactotrophic cells

Mijiddorj, Tselmeg; Kanasaki, Haruhiko; Purwana, Indri N.; Sukhbaatar, Unurjargal; Oride, Aki; Miyazaki, Kohji

doi:10.1507/endocrj.ej12-0093

Cited by 6 publications

(2 citation statements)

References 46 publications

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“…Our study showed that miR-130a-3p targeted ERα and led to a decrease of PRL expression. It has been reported that ligandbound ERs (ERα or ERβ) can form dimers which directly act on the estrogen response elements (EREs) in the promoter regions of estrogen-regulated genes (e.g., PRL) and activate the genes transcription (32). In our study, we found the degradation of ERα induced PRL expression inhibition in GH3 cells, which is consistent with previous reports (33).…”

Section: Discussionsupporting

confidence: 93%

MiR-130a-3p Inhibits PRL Expression and Is Associated With Heat Stress-Induced PRL Reduction

et al. 2020

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MicroRNAs (MiRNAs) play critical roles in the regulation of pituitary function. MiR-130a-3p has previously been found to be down-regulated in prolactinoma, but its roles in prolactin (PRL) regulation and the underlying mechanisms are still unclear. Heat stress has been shown to induce alteration of endocrine hormones and miRNAs expressions. However, there is limited information regarding the emerging roles of miRNAs in heat stress response. In this study, we transfected miR-130a-3p mimic into the pituitary adenoma cells (GH3 cells) to investigate the function of miR-130a-3p in regulating PRL. Our results showed that miR-130a-3p overexpression significantly decreased the PRL expression at both mRNA and protein levels. Subsequently, estrogen receptor α (ERα) was identified as a direct target of miR-130a-3p by bioinformatics prediction, luciferase reporter assay and western blotting assay. Furthermore, the inhibition of ERα caused by estrogen receptor antagonist significantly reduced the PRL expression. Overexpression of ERα rescued the suppressed expression of PRL caused by miR-130a-3p mimic. Besides, we also studied the effect of heat stress on PRL and miRNAs expressions. Interestingly, we found that heat stress reduced PRL and ERα expressions while it increased miR-130a-3p expression both in vitro and in vivo. Taken together, our results indicate that miR-130a-3p represses ERα by targeting its 3'UTR leading to a decrease in PRL expression, and miR-130a-3p is correlative with heat stress-induced PRL reduction, which provides a novel mechanism that miRNAs are involved in PRL regulation.

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Section: Discussionsupporting

confidence: 93%

MiR-130a-3p Inhibits PRL Expression and Is Associated With Heat Stress-Induced PRL Reduction

et al. 2020

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“…15 Progesterone can inhibit basal prolactin promoter activity. 18 Although in some sources it is also mentioned that prolactin is not very responsible for lactogenesis II, other sources say that prolactin is a hormone that plays a role in breast milk synthesis in the early days of postpartum. 19 The mechanism of inhibition is partly mediated by a cross between the progesterone receptor and prolactin signalling to inhibit the induction of milk protein gene expression.…”

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confidence: 99%

Delayed lactogenesis II and unsuccessful exclusive breastfeeding

Pramaningtyas

2019

JKKI

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Breast milk is the main source of nutrition for newborns. Nutrients and bioactive substances that are important for babies are found in breast milk. Cells, anti-infectious and anti-inflammatory agents, growth factors, and prebiotics are bioactive factors in breast milk. 1 These contents are essential to ensure the growth, health, and cognitive development of human infants. 2 World Health Organization recommends mothers to exclusively breastfeed infants for the first six months. Adequate nutritional complementary foods begin at the age of 6 months along with continued breastfeeding until the age of 2 years or more. 3 Exclusive breastfeeding has many benefits for both mother and baby. Benefits for babies include reducing the incidence of respiratory tract infections, otitis media, gastrointestinal infections, allergies, obesity, diabetes, and sudden infant death. Breastfeeding also helps the development of nerves and the intelligence of infants. 4,5 Benefits of breastfeeding for mothers include reducing the incidence of postpartum haemorrhage, accelerating uterine involution, risk of hypertension, type 2 diabetes, metabolic syndrome, and hyperlipidemia. 4,6 Although exclusive breastfeeding is known to provide many benefits for mothers and infants, coverage worldwide only reaches 40%. 3 Coverage of exclusive breastfeeding in America is even only 18.8%. 7 In Indonesia coverage of exclusive breastfeeding reaches 49.8%. 8 Some of the factors that contributed to the achievement of exclusive breastfeeding include the knowledge of maternal factors, maternal body mass index, and delayed lactogenesis II. 9,10 Lactogenesis is the activity of the number of mammary cells to producemilk. It takes a two-stage event, the initiation and activation of the secretory. The first stage of lactogenesis (lactogenesis I) occurs in during the second half of pregnancy. The placenta provides elevated progesterone concentrations that prevent further differentiation. The second stage of lactogenesis (lactogenesis II) begins after shipment with abundant milk manufacturing. The rapid drop in progesterone, as well as the presence of elevated levels of prolactin, cortisol, and insulin, stimulates this stage with the removal of the placenta at delivery. 11 Delayed lactogenesis II is a condition that starts from the failure of activation of the lactogenesis II process. In this condition, breast milk does not come out until 72 hours after postpartum. 12 This condition raises the perception of lack of milk production. In the initial condition after giving birth, nursing mothers who experience this condition have the possibility of having difficulty being able to breastfeed exclusively for up to 6 months. 13,14 Some mothers even stop breastfeeding in the first two weeks. 7 If breast milk does not come out within the first 72 hours, it must immediately look for the causes and how to overcome them. 12 This shows the critical point in the early hours of breastfeeding to be an important period for the success of exclusive breastfeeding. In some studies, di...

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Sex hormone levels and expression of their receptors in lactating and lactating pregnant rats

González-Arenas

Jiménez-Arellano

Morimoto

et al. 2018

The Journal of Steroid Biochemistry and Molecular Biology

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Effects of estradiol and progesterone on prolactin transcriptional activity in somatolactotrophic cells

Cited by 6 publications

References 46 publications

MiR-130a-3p Inhibits PRL Expression and Is Associated With Heat Stress-Induced PRL Reduction

MiR-130a-3p Inhibits PRL Expression and Is Associated With Heat Stress-Induced PRL Reduction

Delayed lactogenesis II and unsuccessful exclusive breastfeeding

Sex hormone levels and expression of their receptors in lactating and lactating pregnant rats

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