Effects of estradiol-17β and progesterone supplementation on in vitro nuclear maturation of canine oocytes

Kim, Min Kyu; Fibrianto, Yuda Heru; Oh, Hyun Ju; Jang, Goo; Kim, Hye Jin; Lee, Kyu Seung; Kang, Sung Keun; Lee, Byeong Chun; Hwang, Woo Suk

doi:10.1016/j.theriogenology.2004.07.019

Cited by 69 publications

(46 citation statements)

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“…Many researchers have reported the in vitro maturation (IVM) of canine oocytes, and the discussion in the literature now focuses on how to improve development. For example, the age of the donor bitch [3], oocyte diameter [4,5], protein [6][7][8][9][10] and hormonal supplementation of maturation media [11][12][13][14][15][16] have all been examined. However, the proportion of MII oocytes remains low (0-43.4%) [9,[15][16][17][18][19][20], and a number of essential issues have yet to be resolved.…”

mentioning

confidence: 99%

Kinetics of Nuclear Status and Kinase Activities During In Vitro Maturation of Canine Oocytes

Suzukamo

Hoshina

Moriya

et al. 2009

Journal of Reproduction and Development

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Abstract. In contrast to those of other mammals, canine oocytes are ovulated at the germinal vesicle (GV) stage and then progress to the metaphase II (MII) stage in the oviduct. In other species, oocytes at the MII are widely used for in vitro fertilization or as recipients in somatic cell nuclear transfer. Many researchers have tried to improve the in vitro maturation (IVM) of canine oocytes. However, the proportion of MII oocytes remains low, resulting in poor efficiency of embryogenesis in vitro. This leads us to the possibility that the in vitro cytoplasmic maturation of canine oocytes is insufficient. Furthermore, the optimal culture period for IVM of canine oocytes is controversial, and physiological evaluation is required to improve canine IVM. We show here the time-dependent changes in mitogen-activated protein kinase (MAPK) and p34 cdc2 kinase activities in canine oocytes during IVM, since it is well known that both MAPK and p34 cdc2 kinase are activated following meiotic progression and show high activities in the MII stage in other species. Immediately after collection from ovaries, most oocytes were arrested at the GV stage, which was maintained until 24 h of culture. At 48 h of culture, more than half of the oocytes had progressed beyond the MI stage. A higher proportion of MII oocytes were observed with 72 h of culture compared with other culture periods. MAPK activity was found to increase in a time-dependent manner and reached a plateau at 72 h of culture. The level of p34 cdc2 kinase activity also increased in a time-dependent manner, with its maximal level observed after 72 h of culture. Activity was decreased with 96 h of culture, although there was no significant difference in the proportion of MII oocytes between 72 and 96 h. Our data thus show that the optimal culture period for IVM of canine oocytes is 72 h because both MAPK and p34 cdc2 kinase showed high activities at that time. Key words: Canine, In vitro maturation, Kinase, Oocyte (J. Reprod. Dev. 55: [116][117][118][119][120] 2009) ocytes in most mammalian species progress to the metaphase II (MII) stage in follicles and are then ovulated into the oviduct; however, canine oocytes are ovulated as immature oocytes at the germinal vesicle (GV) stage. These ovulated canine oocytes require at least 48 h to progress to the MII stage within the oviduct [1,2]. Many researchers have reported the in vitro maturation (IVM) of canine oocytes, and the discussion in the literature now focuses on how to improve development. For example, the age of the donor bitch [3], oocyte diameter [4,5], protein [6][7][8][9][10] and hormonal supplementation of maturation media [11][12][13][14][15][16] have all been examined. However, the proportion of MII oocytes remains low (0-43.4%) [9,[15][16][17][18][19][20], and a number of essential issues have yet to be resolved.One of these issues is the optimal culture period for IVM of canine oocytes. When oocytes are recovered directly from ovarian follicles and cultured in vitro, some progress to the MII stage afte...

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confidence: 99%

Kinetics of Nuclear Status and Kinase Activities During In Vitro Maturation of Canine Oocytes

Suzukamo

Hoshina

Moriya

et al. 2009

Journal of Reproduction and Development

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“…Vet. Zootec., v.63, n.5, p.1054-1059, 2011 concentrações de 1,6ng/mL de progesterona e 31,5pg/mL de estrógeno no soro de cadela em estro, e Kim et al (2005) concluíram a suplementação do meio de cultivo com estrógeno e progesterona, em concentrações de 2,0μg/mL de estrógeno e progesterona após 72 horas de cultivo. Porém, não observaram metáfase II neste tratamento, após 96 horas de cultivo.…”

Section: Methodsunclassified

Efeito do soro de cadela em estro na maturação in vitro de ovócitos caninos

Goretti¹,

Costa²,

Paula³

et al. 2011

Arq. Bras. Med. Vet. Zootec.

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RESUMOAvaliou-se o efeito do soro de cadela em estro na maturação in vitro de ovócitos caninos, utilizando-se 92 ovócitos de cadelas, submetidas à cirurgia eletiva de ovarioisterectomia. Os ovócitos foram selecionados e distribuídos em dois tratamentos: T1 (n = 48), ovócitos cultivados in vitro durante 96 horas utilizando meio base − TCM199 + 5µg/mL de LH + 20µg/mL de FSH − mais 10% de soro inativado de vaca em estro e T2 (n = 44), ovócitos cultivados em meio base mais 10% de soro inativado de cadela em estro. O percentual de ovócitos observados em metáfase I não indicou diferenças (P>0,05) entre T1 (2,1%) e T2 (0,0%), porém a taxa de ovócitos maduros (metáfase II) foi diferente (P<0,05), sendo 27,1% em T1 e 47,7% em T2. O mesmo fato ocorreu com a taxa de cromatina condensada (P<0,01), com 14,6 e 0,0%, respectivamente. Nos ovócitos sem configuração cromossômica, não foram observadas diferenças (P>0,05), sendo 56,3% em T1 e 52,3% em T2. Estes resultados indicam que a adição de soro de cadela em estro no meio de cultivo oferece melhores condições para o desenvolvimento in vitro, quando comparado à de soro de vaca em estro.

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“…The role of oestrus cycle (follicular, luteal or anestrous stages) and the influence of E2 or P4 supplementation on in vitro meiotic competence was investigated by Kim et al (2005). They cultured the canine COC's in different concentration of E2 (0, 0.1, 1.0 or 2.0 mg/ml) and/or P4 (0, 0.5, 1.0 or 2.0 mg/ml) for 72 h. Additionally, they treated the oocytes with the combination of both of these hormones 2 mg/ml E2 and various concentrations of P4 (0, 0.5, 1.0 or 2.0 mg/ml).…”

Section: The Role Of Selected Media Supplements In Canine Oocytes Matmentioning

confidence: 99%

The in vitro culture supplements and selected aspects of canine oocytes maturation

Bukowska¹,

Kempisty²,

Piotrowska³

et al. 2012

Polish Journal of Veterinary Sciences

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The maturation of oocytes is one of the most important steps determining their developmental competence. Due to the low percentage of oocytes of bitches that reach the MII stage, searching for reagents that may stimulate the growth and maturation of oocytes is still present in this species of mammals. The most important media supplements include gonadotropins (LH, FSH, hCG), growth factors (IGF, TGF, EGF, FGF), progesterone and follicular fluid. It is suggested that the supplement of EGF, and/or follicular cells may have an important influence on the percentage of cells that reach the MII stage. Despite plenty of research based on the improvement of bitch oocytes in vitro culture, the results obtained are still unsatisfactory. Moreover, in the long stages of canine oocytes maturation many molecular and morphological modifications (including changes in mitochondria structure and configuration in the cytoplasm) are involved.In this article, the influence of selected media supplements on the efficiency of bitch oocytes in vitro maturation was described. The molecular and morphological modifications during canine oocytes maturation were also considered in the text.

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Effects of estradiol-17β and progesterone supplementation on in vitro nuclear maturation of canine oocytes

Cited by 69 publications

References 24 publications

Kinetics of Nuclear Status and Kinase Activities During In Vitro Maturation of Canine Oocytes

Kinetics of Nuclear Status and Kinase Activities During In Vitro Maturation of Canine Oocytes

Efeito do soro de cadela em estro na maturação in vitro de ovócitos caninos

The in vitro culture supplements and selected aspects of canine oocytes maturation

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