Effects of Enterocytozoon hepatopenaei single-infection or co-infection with Vibrio parahaemolyticus on the hepatopancreas of Penaeus vannamei

Zhu, Bo; Lu, Xiandong; Liu, Yanhong; Wu, Zhongning; HuiFeng, Cai; Jin, Songqing; Zheng, Li; Xie, Jiasong; Li, Xiaobing; Sun, Fuying; Ma, Rongrong; Dong, Qian

doi:10.1016/j.aquaculture.2021.737726

Cited by 9 publications

(3 citation statements)

References 35 publications

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“…The hepatopancreas, as the main target organ of EHP, even plays an important role in regulating the secretion of growth-related hormones in crustaceans [17,37], which is closely related to the growth retardation caused by EHP. Histology analysis observed that the hepatic tubules of EHP-infected shrimp showed varying degrees of atrophy with the duration of infection, and there were vacuoles between the hepatic tubules, accompanied by the shedding of epithelial cells, which is similar to the results of a previous study [38]. At the 20th dpc, the basement membrane of the hepatic tubules in the challenge group was severely disrupted and EHP spores were observed.…”

Section: Discussionsupporting

confidence: 87%

Enterocytozoon hepatopenaei (EHP) Infection Alters the Metabolic Processes and Induces Oxidative Stress in Penaeus vannamei

Cao,

Chen,

Wang

et al. 2023

Animals

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Enterocytozoon hepatopenaei (EHP) is highly contagious and can cause hepatopancreatic microsporidiosis (HPM), which is typically characterized by the slow growth of shrimp. In this study, the differences in histology, metabolism, oxidative stress and growth between healthy and EHP-infected Penaeus vannamei were analyzed using an EHP challenge experiment. Histology showed that EHP caused lesions in the hepatic tubules of P. vannamei, such as hepatic tubular atrophy and epithelial cell shedding, with mature spores. Meanwhile, white feces may appear when the infection is severe. Furthermore, the content of total protein, glycogen, ATP and glucose in the EHP challenge group was significantly reduced. The qPCR results showed that EHP infection changed the expression of key genes in glucose metabolism, among which hexokinase (HK), phosphofructokinase (PFK), pyruvatekinase (PK), citrate synthase (CS) and isocitric dehydrogenase (IDH) were significantly down-regulated, while phosphoenolpyruvate carboxykinase (PEPCK), fructose bisphosphatase (FBP) and glucose-6-phosphatase (G6P) were significantly up-regulated. Obviously, the expression of growth-related genes was disordered. Simultaneously, the antioxidant genes manganese superoxide dismutase (MnSOD), catalase (CAT), glutathione peroxidase (GPX), glutathione-S-transferases (GST) and nuclear factor E2-related factor2 (Nrf2) were up-regulated to varying degrees in the EHP challenge group, and EHP infection induced significant increases in the oxidative damage products lipid peroxide (LPO) and malondialdehyde (MDA). Ultimately, the shrimp weight of the challenge group was 6.85 ± 0.86 g, which was significantly lower than that of the control group (8.95 ± 0.75 g). Taken together, we speculate that EHP changes the substance metabolism and growth process by causing oxidative damage to the hepatopancreas, which may lead to the growth retardation of P. vannamei.

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Section: Discussionsupporting

confidence: 87%

Enterocytozoon hepatopenaei (EHP) Infection Alters the Metabolic Processes and Induces Oxidative Stress in Penaeus vannamei

Cao,

Chen,

Wang

et al. 2023

Animals

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“…The Illumina sequencing and quality assessment, analysis of DEGs, and validation analysis of transcriptomics can be found in previously published literature [ 16 ]. Based on the transcriptome data, this article focuses on analyzing the energy-metabolism-related genes and signaling pathways of low-concentration and high-concentration EHP-infected groups.…”

Section: Resultsmentioning

confidence: 99%

“…Energy-metabolism-related pathway and differentially expressed gene (DEG) analyses were performed using transcriptome data previously obtained by our lab [ 16 ]. In particular, the changes in the oxidative phosphorylation pathway in EHP-infected shrimp were considered.…”

Section: Methodsmentioning

confidence: 99%

The Pathogenic Mechanism of Enterocytozoon hepatopenaei in Litopenaeus vannamei

Ma,

Zhu,

Xiong

et al. 2024

Microorganisms

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Enterocytozoon hepatopenaei (EHP) is a parasite in shrimp farming. EHP mainly parasitizes the hepatopancreas of shrimp, causing slow growth, which severely restricts the economic income of shrimp farmers. To explore the pathogenic mechanism of EHP, the host subcellular construction, molecular biological characteristics, and mitochondrial condition of Litopenaeus vannamei were identified using transmission electron microscopy (TEM), real-time qPCR, an enzyme assay, and flow cytometry. The results showed that EHP spores, approximately 1 μm in size, were located on the cytoplasm of the hepatopancreas. The number of mitochondria increased significantly, and mitochondria morphology showed a condensed state in the high-concentration EHP-infected shrimp by TEM observation. In addition, there were some changes in mitochondrial potential, but apoptosis was not significantly different in the infected shrimp. The qPCR results showed that the gene expression levels of hexokinase and pyruvate kinase related to energy metabolism were both upregulated in the diseased L. vannamei. Enzymatic activity showed hexokinase and lactate dehydrogenase were significantly increased in the shrimp infected with EHP, indicating EHP infection can increase the glycolysis process and decrease the oxidative phosphorylation process of L. vannamei. Previous transcriptomic data analysis results also support this conclusion.

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The effects of CuSO4 on Cryptocaryon irritans tomonts and its potential mechanism

et al. 2022

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Effects of Enterocytozoon hepatopenaei single-infection or co-infection with Vibrio parahaemolyticus on the hepatopancreas of Penaeus vannamei

Cited by 9 publications

References 35 publications

Enterocytozoon hepatopenaei (EHP) Infection Alters the Metabolic Processes and Induces Oxidative Stress in Penaeus vannamei

Enterocytozoon hepatopenaei (EHP) Infection Alters the Metabolic Processes and Induces Oxidative Stress in Penaeus vannamei

The Pathogenic Mechanism of Enterocytozoon hepatopenaei in Litopenaeus vannamei

The effects of CuSO4 on Cryptocaryon irritans tomonts and its potential mechanism

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