Effects of end-stage renal disease and haemodialysis on dendritic cell subsets and basal and LPS-stimulated cytokine production

Agrawal, Sudhanshu; Gollapudi, Pavan; Elahimehr, Reza; Pahl, M. V.; Vaziri, Nosratola D.

doi:10.1093/ndt/gfp580

Cited by 66 publications

(61 citation statements)

References 22 publications

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“…In this regard, we previously described an IL-12 overproduction of monocytes of ESRD patients, as a sign of chronic inflammation in ESRD [30]. The uremic milieu is generally thought to be immunosuppressive, not only in regard to maturation or function [28,29,31], but also in regard to decreased numbers of circulating DC during ESRD, further enhanced by the inflammatory stimulus during hemodialysis [32]. This led us and others [28,29] to the conclusion that the removal of uremic toxins by a more effective dialysis treatment might ameliorate DC function, thus providing a better outcome of patients undergoing hemodialysis.…”

Section: Discussionmentioning

confidence: 96%

Differentiation of Monocyte Derived Dendritic Cells in End Stage Renal Disease is Skewed Towards Accelerated Maturation

Dopheide¹,

Zeller²,

Kuhlmann³

et al. 2015

Adv Clin Exp Med

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Background. Dendritic cells (DC) play an important role in the induction of immune responses. Patients with end stage renal disease (ESRD) suffer from chronic inflammation, leading to a secondary, uremic immunodeficiency associated with alterations in monocyte subpopulations with increased proinflammatory capacities. Objectives. The aim of this study was to examine, under isolated conditions, whether alterations in monocyte subpopulations may affect in vitro maturation of dendritic cells (DC) in patients with ESRD, thus allowing us to draw conclusions for the situation in vivo. Material and Methods. Monocytes from 30 patients undergoing hemodialysis (HD) and 15 healthy volunteers were enriched from peripheral blood leukocytes, differentiated into immature DC (iDC) in medium containing IL-4 and GM-CSF, and were induced with LPS to differentiate into mature DC (mDC). Monocyte subpopulations and DC maturation stages were phenotypically characterized using flow-cytometry. Results. Although phenotypically indistinguishable, the number of both iDC and mDC that were generated from uremic monocytes was significantly higher compared to those from healthy controls (p = 0.02 and p = 0.03, respectively). This was associated with an increased number of CD14+ CD16+ monocytes (p = 0.02) and by a higher maturation efficiency of mDC in patients (p = 0.04). Conclusions. A high percentage of CD14+ CD16+ monocytes in patients with ESRD is associated with an increased propensity to differentiate into DC. This indicates that chronic inflammation may substantiate the biased consistence of monocyte subpopulations leading to profound alteration in DC generation and maturation in ESRD (Adv Clin Exp Med 2015, 24, 2, 257-266).

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Section: Discussionmentioning

confidence: 96%

Differentiation of Monocyte Derived Dendritic Cells in End Stage Renal Disease is Skewed Towards Accelerated Maturation

Dopheide¹,

Zeller²,

Kuhlmann³

et al. 2015

Adv Clin Exp Med

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show abstract

“…7,8 ESRD also results in dendritic cell (DC) depletion and dysfunction. [9][10][11] Given the critical role of DCs in regulation of innate and adaptive immunity, DC depletion can contribute to impaired defense against microbial infections and poor response to vaccination in this population. In addition to profoundly affecting the structure and function of innate immunity, CKD also impacts adaptive immunity mainly T and B lymphocyte.…”

Section: Introductionmentioning

confidence: 99%

Lymphocyte depletion and subset alteration correlate to renal function in chronic kidney disease patients

et al. 2015

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“…The main causes of CKD-associated immune deficiency include the following: (1) depletion of dendritic cells, which are the primary antigen-presenting cells 43 ; (2) reduced CD4 þ helper T-lymphocyte/CD8 þ suppressor T-lymphocyte ratio and depletion of naive and central memory T lymphocytes 44 ; (3) diffuse depletion of B lymphocytes, which contributes to impaired antibody production 45,46 ; (4) defective T-cell and natural killer cell proliferation 47,48 ; and (5) depressed phagocytic capacity and increased apoptosis of the neutrophilic PMN. 49,50 The immune system is affected adversely by both iron deficiency and iron overload.…”

Section: Contribution Of Iron Overload To Systemic Inflammation and Imentioning

confidence: 99%

Safety Issues in Iron Treatment in CKD

Vaziri

2016

Seminars in Nephrology

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Effects of end-stage renal disease and haemodialysis on dendritic cell subsets and basal and LPS-stimulated cytokine production

Abstract: ESRD results in significant DC depletion which is largely due to diminished plasmacytoid DC subset. Haemodialysis procedure intensifies DC depletion and impairs LPS-induced TNFalpha production.

Cited by 66 publications

References 22 publications

Differentiation of Monocyte Derived Dendritic Cells in End Stage Renal Disease is Skewed Towards Accelerated Maturation

Differentiation of Monocyte Derived Dendritic Cells in End Stage Renal Disease is Skewed Towards Accelerated Maturation

Lymphocyte depletion and subset alteration correlate to renal function in chronic kidney disease patients

Safety Issues in Iron Treatment in CKD

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