Effects of Enamel Matrix Derivative on the Viability, Cytokine Secretion, and Phagocytic Activity of Human Monocytes

Khedmat, Sedigheh; Hadjati, Jamshid; Iravani, Azita; Nourizadeh, Maryam

doi:10.1016/j.joen.2010.02.032

Cited by 13 publications

(15 citation statements)

References 20 publications

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“…On the other hand, a study by Khedmat et al . found that EMD did not alter the expression of TNF‐α or IL‐1β as assessed by enzyme‐linked immunosorbent assay and did not modify the phagocytic activity of these cells following treatment with EMD. The effect of EMD on osteoclast differentiation was addressed in a study determining the role of transforming growth factor‐beta receptor type 1 (TGF‐βRI) kinase activity in osteoclastogenesis in the murine macrophage cell line RAW246.7 .…”

Section: Effects Of Enamel Matrix Derivative On Specific Cell Typesmentioning

confidence: 88%

Enamel matrix derivative, inflammation and soft tissue wound healing

Miron

Dard

Weinreb

2014

J of Periodontal Research

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Over 15 years have now passed since enamel matrix derivative (EMD) emerged as an agent capable of periodontal regeneration. Following thorough investigation, evidenced-based clinical application is now established for a multitude of clinical settings to promote regeneration of periodontal hard tissues. Despite the large number of studies and review articles written on this topic, no single review has compiled the influence of EMD on tissue inflammation, an area of research that merits substantial attention in periodontology. The aim of the present review was to gather all studies that deal with the effects of EMD on tissue inflammation with particular interest in the cellular mechanisms involved in inflammation and soft tissue wound healing/resolution. The effects of EMD on monocytes, macrophages, lymphocytes, neutrophils, fibroblasts and endothelial cells were investigated for changes in cell behavior as well as release of inflammatory markers, including interleukins, prostaglandins, tumor necrosis factor-α, matrix metalloproteinases and members of the OPG-RANKL pathway. In summary, studies listed in this review have reported that EMD is able to significantly decrease interleukin-1b and RANKL expression, increase prostaglandin E2 and OPG expression, increase proliferation and migration of T lymphocytes, induce monocyte differentiation, increase bacterial and tissue debris clearance, as well as increase fibroplasias and angiogenesis by inducing endothelial cell proliferation, migration and capillary-like sprout formation. The outcomes from the present review article indicate that EMD is able to affect substantially the inflammatory and healing responses and lay the groundwork for future investigation in the field.

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Section: Effects Of Enamel Matrix Derivative On Specific Cell Typesmentioning

confidence: 88%

Enamel matrix derivative, inflammation and soft tissue wound healing

Miron

Dard

Weinreb

2014

J of Periodontal Research

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“…Since monocytes/macrophages play a key role in the healing process by participating in the innate and acquired immune systems, the increase of their phagocytic activity may accelerate wound healing 19,20,21. Therefore in the current study these cells were used to evaluate the cytotoxicity of the experimented materials.…”

Section: Introductionmentioning

confidence: 99%

In vitrocytotoxicity of four calcium silicate-based endodontic cements on human monocytes, a colorimetric MTT assay

et al. 2014

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ObjectivesThis study was performed to evaluate the cytotoxicity of four calcium silicate-based endodontic cements at different storage times after mixing.Materials and MethodsCapillary tubes were filled with Biodentine (Septodont), Calcium Enriched Mixture (CEM cement, BioniqueDent), Tech Biosealer Endo (Tech Biosealer) and ProRoot MTA (Dentsply Tulsa Dental). Empty tubes and tubes containing Dycal were used as negative and positive control groups respectively. Filled capillary tubes were kept in 0.2 mL microtubes and incubated at 37℃. Each material was divided into 3 groups for testing at intervals of 24 hr, 7 day and 28 day after mixing. Human monocytes were isolated from peripheral blood mononuclear cells and cocultered with 24 hr, 7 day and 28 day samples of different materials for 24 and 48 hr. Cell viability was evaluated using an MTT assay.ResultsIn all groups, the viability of monocytes significantly improved with increasing storage time regardless of the incubation time (p < 0.001). After 24 hr of incubation, there was no significant difference between the materials regarding monocyte viability. However, at 48 hr of incubation, ProRoot MTA and Biodentine were less cytotoxic than CEM cement and Biosealer (p < 0.01).ConclusionsBiodentine and ProRoot MTA had similar biocompatibility. Mixing ProRoot MTA with PBS in place of distilled water had no effect on its biocompatibility. Biosealer and CEM cement after 48 hr of incubation were significantly more cytotoxic to on monocyte cells compared to ProRoot MTA and Biodentine.

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“…It was recently shown that EMD might accelerate wound healing by increasing the phagocytic activity of monocytic cells (22). EMD is also reported to induce a process that mimics normal odontogenesis and can thereby serve as a biologically active pulp-dressing agent that specifically induces pulpal wound healing and hard-tissue formation without affecting healthy pulp (23,24).…”

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confidence: 99%