<i>In vitro</i>cytotoxicity of four calcium silicate-based endodontic cements on human monocytes, a colorimetric MTT assay

Khedmat, Sedigheh; Dehghan, Somayyeh; Hadjati, Jamshid; Masoumi, Farimah; Nekoofar, Mohammad Hossein; Dummer, P. M. H.

doi:10.5395/rde.2014.39.3.149

Cited by 25 publications

(25 citation statements)

References 41 publications

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“…Mori et al [17] microscopically observed that the Biodentine inflammatory reaction at rats subcutaneous tissue had a moderate intensity at 7 analysis days, but at 30 days, the reaction was discrete or insignificant, and similar to MTA. These results agree with Khedmat et al [18] that observed similar cytotoxicity from both material on human monocytes, by the MTT assay.…”

Section: Discussionsupporting

confidence: 83%

A new calcium silicate-based material (Biodentine) for filling radicular perforation in an endodontic-periodontal lesion: A case report

Magro¹,

Venção²,

Kuga³

et al. 2014

sjod

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This case report presents an apical radicular perforation management using new calcium silicate-based cement (Biodentine) in a combined endodontic-periodontal lesion. The presence of apical radicular perforation may interfere in the endodontic treatment prognosis. Radicular perforation filling with bioactive cement through endodontic surgery is a possible treatment. This study presents an apical radicular perforation with periodontal involvement, due to alveolar bone loss on the buccal radicular surface from an incorrect intracanal preparation for the fiber post placing. The chosen alternative was a periapical surgery, the perforation was filled with a silicate and calcium chloride bioactive cement (Biodentine; Septodont, Saint-Maur-des-Fosses Cedex, France), and the radicular surface was etched with citric acid, because the access from root canal was impossible. The follow-up was for 8 months, through clinical and radiographic analysis. At the end of the follow-up, radiographic analyses showed the bone healing, and no clinical changes in periodontal probing depth, gingival recession, and the height of the interproximal mesial and distal papillae were observed. The root perforation treatment has a difficult management, especially when the dental root has a simultaneous periodontal commitment. The Biodentine proves to be a promising material for use in these situations.

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Section: Discussionsupporting

confidence: 83%

A new calcium silicate-based material (Biodentine) for filling radicular perforation in an endodontic-periodontal lesion: A case report

Magro¹,

Venção²,

Kuga³

et al. 2014

sjod

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“…Both MTA and BD demonstrated being non-cytotoxic and did not present a significant difference with regard to viability at 24 and 48 h; at 48 h, cells grown in the presence of MTA and BD possessed a higher percentage of viability than the groups treated with other cements, but there were no significant differences between BD and MTA ( p < 0.05) [17–19]. …”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity and Initial Biocompatibility of Endodontic Biomaterials (MTA and Biodentine™) Used as Root-End Filling Materials

Escobar‐García

Aguirre-López

Méndez-González

et al. 2016

BioMed Research International

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Objective. The aim of this study was to evaluate the cytotoxicity and cellular adhesion of Mineral Trioxide Aggregate (MTA) and Biodentine (BD) on periodontal ligament fibroblasts (PDL). Methods. PDL cells were obtained from nonerupted third molars and cultured; MTS cellular profusion test was carried out in two groups: MTA and BD, with respective controls at different time periods. Also, the LIVE/DEAD assay was performed at 24 h. For evaluation of cellular adhesion, immunocytochemistry was conducted to discern the expression of Integrin β1 and Vinculin at 12 h and 24 h. Statistical analysis was performed by the Kruskal-Wallis and Mann-Whitney U tests. Results. MTA and BD exhibited living cells up to 7 days. More expressions of Integrin β1 and Vinculin were demonstrated in the control group, followed by BD and MTA, which also showed cellular loss and morphological changes. There was a significant difference in the experimental groups cultured for 5 and 7 days compared with the control, but there was no significant statistical difference between both cements. Conclusions. Neither material was cytotoxic during the time evaluated. There was an increase of cell adhesion through the expression of focal contacts observed in the case of BD, followed by MTA, but not significantly.

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“…The MTT assay is a well-established method of determining cell viability and has been used in previous studies to test the cytotoxicity of dental adhesives and other dental materials because it is simple, fast, and objective (Costa et al, '99;Issa et al, 2004;Goldberg, 2008;Huang et al, 2010;Kusdemir et al, 2011;Khedmat et al, 2014;Lee et al, 2015). According to the MTT assay, the control group showed the highest odontoblast viability, followed by the SB, IB, XV, AO, CS, and EB groups.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic effects of one‐step self‐etching adhesives on an odontoblast cell line

Lee

Park

et al. 2015

Scanning

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Summary:The aim of this study was to evaluate the cytotoxic effects of one-step self-etching adhesives. Cells from an immortalized mouse odontoblast cell line (MDPC-23) were cultured with six different dental adhesive systems (diluted to concentrations of 0.5% for 4 h): Adper Easy Bond (EB), Xeno V (XV), iBond (IB), AdheSE One (AO), Clearfil SE primer (CS), and Adper Single Bond 2 (SB). MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and flow cytometric apoptosis assays were used to evaluate cell viability and the rate of apoptosis. The odontoblasts were also examined under a scanning electron microscope. While all of the cultures with adhesives showed reduced viability, the viabilities in the IB and SB groups were not significantly different from the control group. Although increased apoptosis rates were observed in all of the cultures with adhesives, the rate in the SB group was not significantly different from the rate in the control. The control group showed the lowest apoptosis rate followed by the SB, AO, IB, EB, XV, and CS groups. When examined under a scanning electron microscope, control odontoblasts and the SB group exhibited relatively large cytoplasmic extensions. In contrast, in the EB and CS groups, fewer fibroblasts remained adhered to the plate surface. Cytoplasmic membrane shrinkage and cell-free areas with residual membrane fragments from dead cells were observed. In conclusion, all cultures with one-step self-etching adhesives showed increased apoptotic activity. SB, an etch-and-rinse adhesive, was comparable to the control group, and CS and EB showed the lowest odontoblast viabilities according to the MTT assay. SCANNING 38:36-42, 2016.

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In vitrocytotoxicity of four calcium silicate-based endodontic cements on human monocytes, a colorimetric MTT assay

Cited by 25 publications

References 41 publications

A new calcium silicate-based material (Biodentine) for filling radicular perforation in an endodontic-periodontal lesion: A case report

A new calcium silicate-based material (Biodentine) for filling radicular perforation in an endodontic-periodontal lesion: A case report

Cytotoxicity and Initial Biocompatibility of Endodontic Biomaterials (MTA and Biodentine™) Used as Root-End Filling Materials

Cytotoxic effects of one‐step self‐etching adhesives on an odontoblast cell line

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