Effects of Electrical Fields on Neuroblastoma (N2A) Cell Differentiation: Preliminary Results

Fernández, Daniel Becerra; García, Pablo Pérez; Martín, María; Daza, Paula; Serrano-Viseas, Juan A.; Huertas, Gloria; Yufera, A.

doi:10.5220/0010320101520159

Cited by 2 publications

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“…2B); in this way we ensure that the counted cells had fully experienced the electrical stimulation delivered. A MATLAB program was developed in order to analyze these images [43], the cited program allows one to click on cells to count them and displays a ruler tool with moving ends to measure the length of neurites (Fig. 2A).…”

Section: N2a Counting Methods and Differentiation Analysismentioning

confidence: 99%

DC electrical stimulation enhances proliferation and differentiation on N2a and MC3T3 cell lines

et al. 2022

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Background Electrical stimulation is a novel tool to promote the differentiation and proliferation of precursor cells. In this work we have studied the effects of direct current (DC) electrical stimulation on neuroblastoma (N2a) and osteoblast (MC3T3) cell lines as a model for nervous and bone tissue regeneration, respectively. We have developed the electronics and encapsulation of a proposed stimulation system and designed a setup and protocol to stimulate cell cultures. Methods Cell cultures were subjected to several assays to assess the effects of electrical stimulation on them. N2a cells were analyzed using microscope images and an inmunofluorescence assay, differentiated cells were counted and neurites were measured. MC3T3 cells were subjected to an AlamarBlue assay for viability, ALP activity was measured, and a real time PCR was carried out. Results Our results show that electrically stimulated cells had more tendency to differentiate in both cell lines when compared to non-stimulated cultures, paired with a promotion of neurite growth and polarization in N2a cells and an increase in proliferation in MC3T3 cell line. Conclusions These results prove the effectiveness of electrical stimulation as a tool for tissue engineering and regenerative medicine, both for neural and bone injuries. Bone progenitor cells submitted to electrical stimulation have a higher tendency to differentiate and proliferate, filling the gaps present in injuries. On the other hand, neuronal progenitor cells differentiate, and their neurites can be polarized to follow the electric field applied.

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Section: N2a Counting Methods and Differentiation Analysismentioning

confidence: 99%

DC electrical stimulation enhances proliferation and differentiation on N2a and MC3T3 cell lines

et al. 2022

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“…Only cells on top of the electrode surface were considered (Figure 2B); in this way we ensure that the counted cells had fully experienced the electrical stimulation delivered. A MATLAB program was developed in order to analyze these images [43], the cited program allows one to click on cells to count them and displays a ruler tool with moving ends to measure the length of neurites (Figure 2A). In the whole process, four steps were necessary to obtain the results: a) rstly, all cells were counted; b) secondly, only differentiated cells were selected; c) thirdly, the images were stabilized in order to do the last pass d) fourthly, neurite length and orientation were measured with the ruler tool.…”

Section: Electrical Stimulation Protocolmentioning

confidence: 99%

DC Electrical Stimulation effects on N2a and MC3T3 cell lines

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Bocio

Scagliusi

et al. 2022

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Background: Electrical stimulation is a novel tool to promote the differentiation and proliferation of precursor cells. In this work we have studied the effects of direct current (DC) electrical stimulation on neuroblastoma (N2a) and osteoblast (MC3T3) cell lines as a model for nervous and bone tissue regeneration, respectively. We have developed the electronics and encapsulation of a proposed stimulation system and designed a setup and protocol to stimulate cell cultures. Methods: Cell cultures were subjected to several assays to assess the effects of electrical stimulation on them. N2a cells were analyzed using microscope images and an inmunofluorescence assay, differentiated cells were counted and neurites were measured. MC3T3 cells were subjected to an AlamarBlue assay for viability, ALP activity was measured, and a real time PCR was carried out.Results: Our results show that electrically stimulated cells had more tendency to differentiate in both cell lines when compared to non-stimulated cultures, paired with a promotion of neurite growth and polarization in N2a cells and an increase in proliferation in MC3T3 cell line.Conclusions: These results prove the effectiveness of electrical stimulation as a tool for tissue engineering and regenerative medicine, both for neural and bone injuries. Bone progenitor cells submitted to electrical stimulation have a higher tendency to differentiate and proliferate, filling the gaps present in injuries. On the other hand, neuronal progenitor cells differentiate, and their neurites can be polarized to follow the electric field applied.

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Effects of Electrical Fields on Neuroblastoma (N2A) Cell Differentiation: Preliminary Results

Cited by 2 publications

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DC electrical stimulation enhances proliferation and differentiation on N2a and MC3T3 cell lines

DC electrical stimulation enhances proliferation and differentiation on N2a and MC3T3 cell lines

DC Electrical Stimulation effects on N2a and MC3T3 cell lines

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