Effects of early intervention with sodium butyrate on lipid metabolism-related gene expression and liver metabolite profiles in neonatal piglets

Yu, Shijie; Ren, Erdu; Xu, Jingya; Su, Yong; Zhu, Weiyun

doi:10.1016/j.livsci.2016.11.013

Cited by 24 publications

(19 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, obese individuals are often characterized by higher fasting plasma TG and TC compared with lean ones [ 25 ]. Here, we reported that oral administration of SCFAs decreased the concentrations of serum TG, TC and HDL-C, which were consistent with the results of previous studies about different SCFAs [ 26 , 27 ]. These indicated that SCFAs might be beneficial to lowering some risk factors for obesity and diabetes.…”

Section: Discussionsupporting

confidence: 92%

“…In this study, SCFAs treatment down-regulated FAS mRNA level in liver, decreased FAS, ACC and PPARσ mRNA levels in longissimus dorsi, inhibited FAS and ACC mRNA levels in abdominal fat, and enhanced CPT-1α mRNA level in liver and abdominal fat. There were some similar changes of muscles and adipose tissues in previous studies [ 26 , 42 ]. In addition, recent study showed that butyrate and other SCFAs increased the rate of lipolysis in vitro [ 43 ].…”

Section: Discussionsupporting

confidence: 84%

See 1 more Smart Citation

Oral administration of short chain fatty acids could attenuate fat deposition of pigs

Jiao

Diao

et al. 2018

PLoS ONE

View full text Add to dashboard Cite

Short chain fatty acids (SCFAs) are the main products of indigestible carbohydrates that are fermented by microbiota in the hindgut. This study was designed to investigate the effects of oral SCFAs administration on the lipid metabolism of weaned pigs. A total of 21 barrows were randomly allocated into three groups, including control group (orally infused with 200 mL physiological saline per day), low dose SCFAs group (orally infused with 200 mL SCFAs containing acetic acid 20.04 mM, propionic acid 7.71 mM and butyric acid 4.89 mM per day), and high dose SCFAs group (orally infused with 200 mL SCFAs containing acetic acid 40.08 mM, propionic acid 15.42 mM and butyric acid 9.78 mM per day). The results showed that the average daily feed intake of SCFAs groups were lower than that of control group (P<0.05). Oral administration of SCFAs decreased the concentrations of triglyceride (TG), total cholesterol (TC), high density lipoprotein-cholesterol and insulin (P<0.05), and increased the leptin concentration in serum (P<0.05). The total fat, as well as TC and TG levels in liver, was decreased by oral SCFAs administration (P<0.05). In addition, SCFAs down-regulated the mRNA expressions of fatty acid synthase (FAS) and sterol regulatory element binding protein 1c (P<0.05), and enhanced the mRNA expression of carnitine palmitoyltransferase-1α (CPT-1α) in liver (P<0.05). SCFAs also decreased FAS, acetyl-CoA carboxylase (ACC) and peroxisome proliferator activated receptor σ mRNA expressions in longissimus dorsi (P<0.05). And in abdominal fat, SCFAs reduced FAS and ACC mRNA expressions (P<0.05), and increased CPT-1α mRNA expression (P<0.05). These results suggested that oral administration of SCFAs could attenuate fat deposition in weaned pigs via reducing lipogenesis and enhancing lipolysis of different tissues.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 84%

Oral administration of short chain fatty acids could attenuate fat deposition of pigs

Jiao

Diao

et al. 2018

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Besides, SREBP-1c enhances the transcription of targeted genes that encode the enzymes of cholesterol biosynthesis and uptake [35]. Consistent with previous studies [36], we found that SCFAs reduced the mRNA expressions of FAS, ACC and/or SREBP-1c, which could attenuate adipogenesis and cholestrol synthesis. Moreover, suppression of Fiaf increased LPL activity in adipocytes, thereby increasing triglyceride storage [37].…”

Section: Discussionsupporting

confidence: 92%

Acetate, Propionate and Butyrate Reduce Appetite and Fat Accumulation in Mice via Modulating Relevant Genes and Hormones

Chen¹,

Jiao²,

Yu³

et al. 2020

Preprint

View full text Add to dashboard Cite

Acetate, propionate and butyrate, three of the most common short chain fatty acids (SCFAs), can be produced when some non-digestible carbohydrates enter the large intestine and undergo bacterial fermentation. This study was designed to investigate the effects of these three SCFAs on appetite regulation and lipid metabolism, and to what extent appetite contributed to the beneficial influences of SCFAs. In a 35-day study, a total of 48 C57BL/6 male mice were randomly allocated into six groups : (1) control; (2) 5% sodium acetate; (3) 5% sodium propionate; (4) 5% sodium butyrate; (5) pair fed 1; (6) pair fed 2. The results showed that sodium acetate reduced serum triglyceride, free fatty acids, glucose and interleukin (IL) 6 levels (P < 0.05), increased serum glucagon-like peptide 1 and leptin levels (P < 0.05), down-regulated the mRNA expressions of fatty acid synthase, peroxisome proliferator activated receptor and lipoprotein lipase (P < 0.05), and up-regulated the mRNA expressions of fasting induced adipose factor, nuclear respiratory factor 1, mitochondrial transcription factor A, tumor necrosis factor receptor superfamily member 9, cytochromec oxidase IV and free fatty acid receptor 2 (P < 0.05). Sodium propionate also reduced serum IL-1β level (P < 0.05), increased serum peptide YY level (P < 0.05), down-regulated the mRNA expressions of acetyl-CoA carboxylase and sterol regulatory element binding protein 1c (P < 0.05), and up-regulated the mRNA expression of transmembrane protein 26 (P < 0.05). Besides, Sodium butyrate decreased average daily feed intake (P < 0.05), down-regulated the mRNA expression of myosin heavy-chain (MyHc) Ⅱb (P < 0.05), and up-regulated the mRNA expressions of lipase hormone-sensitive, MyHC Ⅱa and carnitine palmitoyltransferase-1α (P < 0.05). Moreover, the metabolic benefits of SCFAs were partly attributed to the reduction of feed intake. Taken together, SCFAs could reduce appetite and fat accumulation via modulating relevant genes and hormones, which might further illustrate the potential mechanisms that underlay the impacts of SCFAs on lipid homeostasis and body weight control.

show abstract

“…In addition, we found that oral infusion of SCFAs markedly downregulated the mRNA expressions of FAS, ACC, and SREBP-1C in liver. Consistently, previous studies reported similar results in liver, longissimus dorsi, and adipose tissues of conventional pigs [9,10,41]. Remarkably, FAS is the pivotal enzyme that catalyzes fatty acid synthesis [42].…”

Section: Discussionsupporting

confidence: 86%

Short-chain Fatty Acids can Improve Lipid and Glucose Metabolism Independently of the Gut Microbiota

Zhang¹,

Yu²,

Sun

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Previous research has shown that exogenously short-chain fatty acids (SCFAs) infusion attenuated the body fat deposition in both mice, humans, and pigs. However, limited studies have evaluated the effects of SCFAs on lipid and glucose metabolism independently of the gut microbiota. Therefore, the present study was conducted to investigate the effects of exogenous infusion of SCFAs on lipid and glucose metabolism in a germ-free (GF) pig model. Methods: Twelve hysterectomy-derived newborn pigs were reared in six sterile isolators. All pigs were hand-fed Co60-γ-irradiated sterile milk powder for 21 days and switched from liquid to sterile solid feed for another 21 days. In the second 21-days period, GF pigs (n = 6) were orally infused with 25 mL/kg sterile saline per day, FA pigs (n = 6) were orally infused with 25 mL/kg SCFAs mixture (acetic, propionic, and butyric acids, 45, 15, and 11 mM, respectively) per day.Results: Orally infused with SCFAs tended to increase the concentration of adiponectin in serum, the activity of CPT-1 in longissimus dorsi, and the ANGPTL4 mRNA expression in colon (P < 0.10). Meanwhile, the mRNA abundances of ACC, FAS, and SREBP-1C in liver and CD36 in longissimus dorsi were decreased (P < 0.05), and the concentrations of ATP, ADP, or AMP in liver and longissimus dorsi were reduced (P < 0.05) in FA group. Besides, the mRNA expressions of PGC-1α in liver and LPL in longissimus dorsi were tended to (P < 0.10) increase and downregulate in FA group respectively. Moreover, the protein level of GPR43 was tended to increase (P < 0.10), and ACC was tended to decrease (P < 0.10), while the p-AMPK/T-AMPK ratio (P < 0.05) was upregulated in liver of FA group. Also, oral administration of SCFAs upregulated the mRNA expressions of SLC2a and GYS2 in liver (P < 0.05). Furthermore, the metabolic pathway associated with the biosynthesis of unsaturated fatty acids was significantly promoted (P < 0.05) by orally infused with SCFAs.Conclusions: Short-chain fatty acids could attenuate fat deposition and to some extent improve glucose control, and were occur independently of the gut microbiota.

show abstract

Effects of early intervention with sodium butyrate on lipid metabolism-related gene expression and liver metabolite profiles in neonatal piglets

Cited by 24 publications

References 34 publications

Oral administration of short chain fatty acids could attenuate fat deposition of pigs

Oral administration of short chain fatty acids could attenuate fat deposition of pigs

Acetate, Propionate and Butyrate Reduce Appetite and Fat Accumulation in Mice via Modulating Relevant Genes and Hormones

Short-chain Fatty Acids can Improve Lipid and Glucose Metabolism Independently of the Gut Microbiota

Contact Info

Product

Resources

About