Effects of drying temperature on viability of macaw palm (Acrocomia aculeata) zygotic embryos

Crabwood, a popular name of several pantropical timber species, has become increasingly important for its seed oil of pharmaceutical and cosmetic use. Due to the recalcitrant character of the seeds, plantations are limited. The aim of this study was to develop a tetrazolium (TZ) staining protocol and validate viability staining with germination tests. Seed preparation was standardized in order to localize and cut the tiny embryonic axis longitudinally, which is inserted in the fused cotyledonal seed mass. Staining intensity was determined by testing different concentrations of TZ solution (0.05, 0.10, 0.25 and 0.50%) at three temperatures (25, 30 and 35°C) during a period of up to 6 h. Taking into account the large seed size, costs and working time, a solution of 0.10% TZ at 30°C for 3 h was considered appropriate for both species. The method was validated with seeds of different qualities (between 0 and 90% germination capacity), obtained by controlled drying over a fan. The desiccation revealed initial damage near the seed surface close to the radicle meristem. Images of the stained seeds were classified in four viability classes and were re-evaluated with the germination results (radicle ≥ 0.5 cm and normal seedlings). The proposed method for tetrazolium staining was effective in assessing seed viability of both species.

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Alternative tube caps on in vitro growth of Orbignya oleifera Burret.: An Arecaceae native cerrado domain

Leite¹,

Faria²,

Pereira³

et al. 2016

Afr. J. Biotechnol.

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The babassu (Orbignya oleifera Burret) is a palm that contains oilseeds whose biomass can be used for biofuel production. Due to difficulties in germinating the species, in vitro cultivation techniques are sought as a viable alternative to expand studies on germination. The objective of this study was to evaluate different types of tube caps for in vitro growth of babassu seedlings. Thus, zygotic embryos were inoculated in tubes containing MS medium with a 50% salt concentration, which were sealed with diferent caps, a cotton plug, plastic cap, PVC film and plastic cap with PVC film. During the in vitro culture, the mean length of seedlings, the formation of the cotyledon petiole and root, as well as oxidation of the explants were evaluated. The cotton plug exhibited a positive effect on the initial steps of in vitro cultivation, which promoted more vigorous plants with greater mean lengths; however, its effect decreased as the cultivation time increased. The cotton plug is an alternative and eight-fold less expensive than a conventional plug, which favors early in vitro growth of O. oleifera Burret.

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Effects of drying temperature on viability of macaw palm (Acrocomia aculeata) zygotic embryos

Cited by 3 publications

References 12 publications

Accelerated ageing and subsequent imbibition affect seed viability and the efficiency of antioxidant system in macaw palm seeds

Accelerated ageing and subsequent imbibition affect seed viability and the efficiency of antioxidant system in macaw palm seeds

Seed quality evaluation by tetrazolium staining during a desiccation study of the recalcitrant seeds of Carapa guianensis Aubl. and Carapa surinamensis Miq. - Meliaceae

Alternative tube caps on in vitro growth of Orbignya oleifera Burret.: An Arecaceae native cerrado domain

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