Effects of dorsal aorta cannulation on the stress response of channel catfish (Ictalurus punctatus)

Mazik, Patricia M.; Plakas, Steven M.; Stehly, Guy R.

doi:10.1007/bf00004308

Cited by 12 publications

(7 citation statements)

References 15 publications

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“…These result indicate that force‐fed rainbow trout might recover from the stress of dorsal aorta cannulation within 48 h of the operation, and the dorsal aorta cannulation would allow repeated sampling on the same individual fish to study nutrient metabolism in the blood circulation. This is in line with findings in rainbow trout (Brown, Eales & Hara 1986) and channel catfish Ictalurus punctatus (Ratinesque) (Mazik, Plakas & Stehly 1994) subjected to dorsal aorta cannulation. The pattern of plasma free amino acid concentrations from force‐fed rainbow trout with dorsal aorta cannulation may be useful in determining the optimum blood sampling time and evaluating protein quality and essential amino acid requirements.…”

Section: Discussionsupporting

confidence: 91%

The patterns of plasma free amino acids after force-feeding in rainbow trout Oncorhynchus mykiss (Walbaum) with and without dorsal aorta cannulation

Bai

Park

et al. 2001

Aquaculture Research

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Two experiments were conducted to compare the patterns of plasma free amino acid concentrations after force‐feeding in rainbow trout Oncorhynchus mykiss (Walbaum) with and without dorsal aorta cannulation. In the first experiment, 35 rainbow trout averaging 504 ± 7.8 g (mean ± SD) were divided into seven groups of five fish each. After 48 h starvation, a group of fish was anaesthetized and blood samples were taken at one of the following time periods: 0, 4, 8, 12, 24, 36 and 48 h after feeding. In the second experiment, five dorsal aorta cannulated rainbow trout averaging 511 ± 6.2 g (mean ± SD) were kept in a cage. After 48 h starvation, the fish were anaesthetized and blood samples were taken from the same fish at 0, 4, 8, 12, 24, 36 and 48 h after feeding. In the first experiment, the concentration of all plasma free amino acids except histidine and glycine peaked at 4 h and returned to the basal level 24 h after feeding. In the second experiment, the concentration of all plasma free amino acids except isoleucine, leucine, phenylalanine and tryptophan also peaked at 4 h and returned to the basal level 24 h after feeding. These results showed that the pattern of plasma free amino acid concentrations from fish with and without dorsal aorta cannulation were similar.

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Section: Discussionsupporting

confidence: 91%

The patterns of plasma free amino acids after force-feeding in rainbow trout Oncorhynchus mykiss (Walbaum) with and without dorsal aorta cannulation

Bai

Park

et al. 2001

Aquaculture Research

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“…The use of cannulation to obtain baseline values may result in persistent alterations to primary and secondary stress responses depending on the species (Duthie & Tort, 1985;Gamperl et al, 1994a,b;Mazik et al, 1994). The negative effects of chronically elevated cortisol on the performance of fish is acknowledged by the aquaculture industry, and selective breeding to reduce stress responses has been proposed (Pickering, 1992).…”

Section: Discussionmentioning

confidence: 99%

Primary and secondary stress responses to line capture in the blue mao mao

Lowe

Wells

1996

Journal of Fish Biology

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Primary and secondary stress responses were measured in wild Scorpis violaceus subjected to burst swimming from angling. Fish were blood sampled from 20 s to 30 min after hooking. Consequent rises in plasma adrenaline (14-316 nmol l 1 ), noradrenaline (25-345 nmol l 1 ), and cortisol (0·4-197 ng ml 1 ) correlated with time since capture, and plasma lactate (0·1-12·2 mmol l 1 ) reflected work done during intense exercise. Haemoglobin concentration and haematocrit also increased with exercise, and erythrocyte swelling occurred. Wild S. violaceus demonstrated a spontaneity and intensity of exercise not seen in fish acclimatized to aquarium conditions. By contrast, the stress responses of fish in captivity, despite careful husbandry, differed qualitatively and quantitatively from those in the wild. Cannulated fish had higher resting plasma cortisol concentrations (61·9 9·5 ng ml 1 ) than did rapidly caught wild fish (<5 ng ml 1 ) and these values were not significantly changed with burst swimming. Catecholamine secretion, possibly suppressed by cortisol, was insufficient to cause erythrocyte swelling. Erythrocyte nucleotides do not play a role in exercise, but are elevated in captive fish. It is hypothesized that primary endocrine responses are triggered by higher cortical processing of sensory information which is fundamentally different in the natural environment. 1996 The Fisheries Society of the British Isles

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“…Although the surgical implantation of a cannula alleviates the stress effects of repeated netting, handling, and anesthesia, the presence of a cannula may still impose stress on the animal (4). Considerable effort has been placed into investigating the effect of cannulation and/or confinement on fish, specifically through describing the time-course changes on indicators of stress such as circulating levels of catecholamines and cortisol (e.g., 38,42,66). Previous studies have shown plasma cortisol levels in rainbow trout peak at 24 h postsurgery (after which levels steadily decline; 3, 19, 21).…”

Section: Transcriptomic Response Of the Rbc To Heat Stressmentioning

confidence: 99%

Transcriptome responses to heat stress in the nucleated red blood cells of the rainbow trout (Oncorhynchus mykiss)

Lewis

Hori

Rise

et al. 2010

Physiological Genomics

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The retention of a nucleus in the mature state of fish red blood cells (RBCs) and the ability to easily collect and manipulate blood in nonterminal experiments make blood an ideal tissue on which to study the cellular stress response in fish. Through the use of the cGRASP 16K salmonid microarray, we investigated differences in RBC global gene transcription in fish held under control conditions (11 degrees C) and exposed to heat stress (1 h at 25 degrees C followed by recovery at 11 degrees C). Repeated blood sampling (via a dorsal aorta cannula) enables us to examine the individual stress response over time. Samples were taken preheat stress (representing individual control) and at 4 and 24 h postheat stress (representing early and late transcriptional regulation). Approximately 3,000 microarray features had signal above threshold when hybridized with RBC RNA-derived targets, and cannulation did not have a detectable effect on RBC mRNA expression at the investigated time points. Genes involved in the stress response, immune response, and apoptosis were among those showing the highest dysregulation during both early and late transcriptional regulation. Additionally, genes related to the differentiation and development of blood cells were transcriptionally upregulated at the 24 h time point. This study provides a broader understanding of the mechanisms underpinning the stress response in fish and the discovery of novel genes that are regulated in a stress specific manner. Moreover, salmonid transcripts that are consistently dysregulated in blood in response to heat stress are potential candidates of nonlethal biomarkers of exposure to this particular stressor.

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Effects of dorsal aorta cannulation on the stress response of channel catfish (Ictalurus punctatus)

Cited by 12 publications

References 15 publications

The patterns of plasma free amino acids after force-feeding in rainbow trout Oncorhynchus mykiss (Walbaum) with and without dorsal aorta cannulation

The patterns of plasma free amino acids after force-feeding in rainbow trout Oncorhynchus mykiss (Walbaum) with and without dorsal aorta cannulation

Primary and secondary stress responses to line capture in the blue mao mao

Transcriptome responses to heat stress in the nucleated red blood cells of the rainbow trout (Oncorhynchus mykiss)

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