Effects of dopants in the imaging of mouse brain by desorption electrospray ionization/post‐photoionization mass spectrometry

Wu, Lisheng; Qi, K. H.; Xu, Minggao; Liu, Chengyuan; Pan, Yang

doi:10.1002/jms.4813

Cited by 5 publications

(11 citation statements)

References 67 publications

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“…The MS images of some representative constituents by imprint DESI/PI and DESI are compared in Figure C, and better images were obtained by imprint DESI/PI for all but choline ( m / z 104.11) and PC (40:6) ( m / z 813.49). The suppression of MS signals of highly polar choline and PC (40:6) in imprint DESI/PI was speculated to be mainly due to the addition of nonpolar toluene in the spray solvent, which is also observed in previous reports. , As imaged by imprint DESI, choline ( m / z 104.11) was mainly distributed in the veins and tails of sage leaves, and PC (40:6) ( m / z 813.49) was mainly distributed in the midvein and lateral veins. Imprint DESI/PI enabled imaging of metabolites, including geroquinol ( m / z 247.17), dehydroabietic acid ( m / z 301.22), abietic acid ( m / z 303.23), cirsimaritin ( m / z 315.08), carnosol ( m / z 331.19), carnosic acid ( m / z 333.21), ballotenic acid ( m / z 335.22), epirosmanol/rosmanol ( m / z 347.19), methyl carnosate ( m / z 347.22), glochidone ( m / z 423.36), lupeol ( m / z 427.39), ursolic acid/oleanolic acid ( m / z 455.35), glabrolide ( m / z 469.33), and diacylglycerol DAG (36:6) ( m / z 613.48).…”

Section: Resultssupporting

confidence: 78%

“…Sages belong to the Lamiaceae family, which is rich in terpenoids with specific biological functions. Most of the metabolites in sage leaves were detected in the form of [M + H] + in imprint DESI/PI via a proton-transfer reaction in the dopant-assisted PI process, and the photon-induced post-ionization reactions have been discussed elsewhere in detail . As shown in Figure C, dehydroabietic acid and abietic acid were detected and imaged by imprint DESI/PI as [M + H] + ions at m / z 301.22 and 303.23, respectively, and they are distributed in the whole sage leaf except veins.…”

Section: Resultsmentioning

confidence: 98%

“…To overcome this issue, post-photoionization (PI) coupled with DESI, named DESI/PI, was developed by our group for the simultaneous imaging of both polar and nonpolar species in biological tissues in 2019 . The unionized molecules desorbed by DESI can be post-ionized by photon-induced ion-molecule reactions, which enabled more than 2 orders of magnitude higher signal intensities for certain neutral metabolites in DESI/PI. The composition of the spray solvent was also carefully studied for enhanced analysis and imaging of various metabolites in DESI/PI. , …”

Section: Introductionmentioning

confidence: 99%

“…The unionized molecules desorbed by DESI can be post-ionized by photon-induced ion-molecule reactions, which enabled more than 2 orders of magnitude higher signal intensities for certain neutral metabolites in DESI/PI. The composition of the spray solvent was also carefully studied for enhanced analysis and imaging of various metabolites in DESI/PI. , …”

Section: Introductionmentioning

confidence: 99%

“…The composition of the spray solvent was also carefully studied for enhanced analysis and imaging of various metabolites in DESI/PI. 27,28 In this work, imprint in combination with DESI/PI was developed for the simultaneous mapping of various metabolites, including terpenoids, flavonoids, glycosides, phenolics, and alkaloids, in plant materials. The MSI performance of DESI/PI and DESI was systematically compared for fresh sage leaves, ginkgo leaves, and tea leaves in both positive and negative ion modes.…”

Section: ■ Introductionmentioning

confidence: 99%

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Enhanced Coverage and Sensitivity of Imprint DESI Mass Spectrometry Imaging for Plant Leaf Metabolites by Post-photoionization

Liu

et al. 2022

Anal. Chem.

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Plant metabolites exhibit a variety of different chemical properties, physiological activities, and biological functions. However, untargeted imaging of highly diverse metabolic profiles is still a great challenge. Here, metabolites in plant leaves were imaged via imprint, followed by desorption electrospray ionization/post-photoionization (imprint DESI/PI) mass spectrometry imaging. In contrast to the traditional imprint DESI method, quite a few metabolites, such as terpenoids, flavonoids, glycosides, alkylphenols, amino acids, phenolic acids, tannins, and lipids, in fresh sage leaves, ginkgo leaves, and tea leaves were well detected and imaged by imprint DESI/PI. More than 80 metabolites were additionally identified, and more than 1 order of magnitude higher signal intensities were obtained for most metabolites in the negative ion mode. By virtue of the significant improvement of coverage and sensitivity of PI, the catechin biosynthesis network in fresh tea leaves could be clearly illustrated, indicating the potential applicability of imprint DESI/PI in exploring the sites and pathways of plant metabolic conversion.

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Section: Resultssupporting

confidence: 78%

Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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Enhanced Coverage and Sensitivity of Imprint DESI Mass Spectrometry Imaging for Plant Leaf Metabolites by Post-photoionization

Liu

et al. 2022

Anal. Chem.

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Applications of ambient ionization mass spectrometry in 2022: An annual review

Rankin‐Turner

Sears

Heaney

2023

Analytical Science Advances

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The development of ambient ionization mass spectrometry (AIMS) has transformed analytical science, providing the means of performing rapid analysis of samples in their native state, both in and out of the laboratory. The capacity to eliminate sample preparation and pre‐MS separation techniques, leading to true real‐time analysis, has led to AIMS naturally gaining a broad interest across the scientific community. Since the introduction of the first AIMS techniques in the mid‐2000s, the field has exploded with dozens of novel ion sources, an array of intriguing applications, and an evident growing interest across diverse areas of study. As the field continues to surge forward each year, ambient ionization techniques are increasingly becoming commonplace in laboratories around the world. This annual review provides an overview of AIMS techniques and applications throughout 2022, with a specific focus on some of the major fields of research, including forensic science, disease diagnostics, pharmaceuticals and food sciences. New techniques and methods are introduced, demonstrating the unwavering drive of the analytical community to further advance this exciting field and push the boundaries of what analytical chemistry can achieve.

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Crown ether dopant to reduce ion suppression and improve detection in the liquid microjunction surface sampling probe

Metwally,

Yu,

Theriault

et al. 2024

Rapid Comm Mass Spectrometry

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RationaleSodium and potassium are required in agar media for the growth of some microorganisms (e.g., marine bacteria). However, alkali cations are a significant source of contamination for mass spectrometry causing ion suppression and adduct formation. Conventionally, salts can be removed before mass spectrometric analysis with appropriate and often lengthy sample preparation. The direct mass spectrometric sampling of bacterial colonies grown on agar media seeks to minimize or eliminate sample preparation to improve workflow. However, this may exacerbate ion suppression and contamination since these metal cations will degrade spectral quality and limit the rapid profiling of microbial metabolites. Different approaches are needed to sequester sodium and potassium ions to minimize unwanted background interferences. Herein, we use crown ethers (CEs) in combination with a liquid microjunction surface sampling probe (LMJ‐SSP) to directly sample the surface of the bacterial colonies from two marine bacteria species (Pseudoalteromonas rubra DSM6842 and Pseudoalteromonas tunicata DSM 14096). CEs (e.g., 18‐crown‐6 or 15‐crown‐5) are added to the carrier solvent of the LMJ‐SSP, the chemical noise is reduced, and spectra are easier to interpret.MethodsThe liquid microjunction formed at the tip of LMJ‐SSP was used to directly touch bacterial colonies on agar. The carrier solvent was either methanol (100%) or methanol: H2O (50:49.9%) with or without 0.01% CEs. Information‐theoretic measures are employed to investigate qualitative changes between spectra before and after adding CEs.ResultsOur work demonstrates the capability of CEs to reduce background interferences within the direct profiling of bacterial colonies from agar plates. The data obtained from both P. rubra DSM6842 and P. tunicata DSM 14096 show that CEs can be used to mitigate the salty background and improve compound detection.ConclusionOur approach can be implemented in natural product discovery using LMJ‐SSP to allow fast and accurate detection of interesting/novel compounds.

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Effects of dopants in the imaging of mouse brain by desorption electrospray ionization/post‐photoionization mass spectrometry

Cited by 5 publications

References 67 publications

Enhanced Coverage and Sensitivity of Imprint DESI Mass Spectrometry Imaging for Plant Leaf Metabolites by Post-photoionization

Enhanced Coverage and Sensitivity of Imprint DESI Mass Spectrometry Imaging for Plant Leaf Metabolites by Post-photoionization

Applications of ambient ionization mass spectrometry in 2022: An annual review

Crown ether dopant to reduce ion suppression and improve detection in the liquid microjunction surface sampling probe

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