Effects of DNMT and HDAC Inhibitors (RG108 and Trichostatin A) on NGF-induced Neurite Outgrowth and Cellular Migration

Tilki, Elif Kaya; Dikmen, Miriş; Öztürk, Yusuf

doi:10.3923/ijp.2016.351.360

Cited by 8 publications

(6 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Before analysis, media containing concentration groups were removed, and wells were imaged for wound diameter variation using a Leica DM 300 light microscope (x4). The cells were then labeled with an immunofluorescent dye (Hoechst 33258), and the fluorescence absorption was measured and plotted with the Cytation 3 cell imaging multimode reader …”

Section: Methodsmentioning

confidence: 99%

“…The cells were then labeled with an immunofluorescent dye (Hoechst 33258), and the fluorescence absorption was measured and plotted with the Cytation 3 cell imaging multimode reader. 49 …”

Section: Methodsmentioning

confidence: 99%

“…The cells were then labeled with an immunofluorescent dye (Hoechst 33258), and the fluorescence absorption was measured and plotted with the Cytation 3 cell imaging multimode reader. 49 Statistical Analysis. Each experiment's data was entered into GraphPad Prism 7.0 software, and then the replicates were averaged, and standard deviations were determined.…”

Section: ■ Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of Cell Proliferation and Wound Healing Effects of Vitamin A Palmitate-Loaded PLGA/Chitosan-Coated PLGA Nanoparticles: Preparation, Characterization, Release, and Release Kinetics

2023

View full text Add to dashboard Cite

In this study, vitamin A palmitate (VAP)-loaded poly(lactic-co-glycolic acid) (PLGA)/chitosan-coated PLGA nanoparticle (NP) systems were prepared by the nanoprecipitation technique. The prepared systems were characterized by parameters such as particle size, polydispersity index (PDI), ζ-potential, encapsulation efficiency, in vitro dissolution, and release kinetic study. Then, the cytotoxicity and wound healing profiles of the designed NP formulations in HaCaT (human keratinocyte skin cell lines) were determined. The particle size of VAP-loaded NPs was obtained between 196.33 ± 0.65 and 669.23 ± 5.49 nm. PDI data proved that all NPs were prepared as high quality and monodisperse. While negative ζ-potential values of Blank-NP-1 and NP-1 encoded PLGA NP formulations were obtained, positive ζ-potential was obtained in chitosan-coated NPs. In vitro release studies of NPs observed rapid dissolution in the first 1–6 h, but prolonged dissolution of VAP after rapid dissolution. As a result of cell culture studies and wound healing activity studies, it was determined that NP-7 was the most effective. It was thought that the reason for this was that the NP-7 coded formulation was a chitosan-coated PLGA nanoparticle with the smallest particle size, and it was concluded that the efficiency of VAP was increased with its nanoparticle structure. This study demonstrated the similar wound healing effects of VAP-loaded nanoparticle systems, in particular NP-7, which increases keratinocyte cell proliferation at lower concentrations (10 μg·mL–1) than vitamin A alone (100 μg·mL–1). VAP-loaded nanocarriers that can be used in the pharmaceutical industry have been successfully produced and the results obtained have been evaluated as promising for this industry.

show abstract

Section: Methodsmentioning

confidence: 99%

“…The cells were then labeled with an immunofluorescent dye (Hoechst 33258), and the fluorescence absorption was measured and plotted with the Cytation 3 cell imaging multimode reader. 49 …”

Section: Methodsmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of Cell Proliferation and Wound Healing Effects of Vitamin A Palmitate-Loaded PLGA/Chitosan-Coated PLGA Nanoparticles: Preparation, Characterization, Release, and Release Kinetics

2023

View full text Add to dashboard Cite

show abstract

“…5 μl Annexin V and 2 μl PI working solution were added to the centrifuge tubes and binded with 100 μl Annexin V binding buffer. After the samples were incubated for 30 minutes on ice and in the dark, 400 μl of Annexin V binding buffer was added to each tube and the samples were measured and analyzed in a BD Accuri™ C6 flow cytometry device [ 59 ].…”

Section: Methodsmentioning

confidence: 99%

Neuroprotective effects of some epigenetic modifying drugs’ on Chlamydia pneumoniae-induced neuroinflammation: A novel model

Tilki

Dikmen

2021

PLoS ONE

Self Cite

View full text Add to dashboard Cite

Chlamydia pneumoniae (Cpn) is a gram-negative intracellular pathogen that causes a variety of pulmonary diseases, and there is growing evidence that it may play a role in Alzheimer’s disease (AD) pathogenesis. Cpn can interact functionally with host histones, altering the host’s epigenetic regulatory system by introducing bacterial products into the host tissue and inducing a persistent inflammatory response. Because Cpn is difficult to propagate, isolate, and detect, a modified LPS-like neuroinflammation model was established using lyophilized cell free supernatant (CFS) obtained from infected cell cultures, and the effects of CFS were compared to LPS. The neuroprotective effects of Trichostatin A (TSA), givinostat, and RG108, which are effective on epigenetic mechanisms, and the antibiotic rifampin, were studied in this newly introduced model and in the presence of amyloid beta (Aβ) 1–42. The neuroprotective effects of the drugs, as well as the effects of CFS and LPS, were evaluated in Aβ-induced neurotoxicity using a real-time cell analysis system, total ROS, and apoptotic impact. TSA, RG108, givinostat, and rifampin all demonstrated neuroprotective effects in both this novel model and Aβ-induced neurotoxicity. The findings are expected to provide early evidence on neuroprotective actions against Cpn-induced neuroinflammation and Aβ-induced neurotoxicity, which could represent a new treatment option for AD, for which there are currently few treatment options.

show abstract

“…The measured absorbance directly correlates with the number of viable cells. Cell viability rates were expressed as a percentage of the solvent control value (0.1 % DMSO in the medium) [30,31] .…”

Section: Lipopolysaccharide (Lps) Inflammation Modelmentioning

confidence: 99%

Evaluation of Real Time Cell Proliferation, Anti-Inflammatory and Wound Healing Potential of Helenalin on HaCaT Keratinocytes Treated with Lipopolysaccharide Stimulated Monocytes

Yüksel¹,

Dikmen²,

Cantürk³

2021

ijps

View full text Add to dashboard Cite

Yuksel et al.: Cell Proliferation, Anti-Inflammatory and Wound Healing Potential of Helenalin Arnica montana, of the family Asteraceae, is used for its anti-inflammatory and wound healing properties, especially for bruises, tissue injuries and other traumas. However, its cellular and molecular mechanisms are not yet fully known. Its main active ingredient is helenalin, a sesquiterpene lactone, known as one of the main active ingredients of Asteraceae species. The aim of this study was to investigate cell proliferation, wound healing and anti-inflammatory activity of helenalin on human keratinocyte cells treated with lipopolysaccharide stimulated human monocytic cell line. Human keratinocyte cells were treated with different helenalin concentrations (0.095-50 μM) to determine toxic and nontoxic concentrations. Then, the effects of nontoxic (0.02 and 0.2 μM) and toxic (2 μM) helenalin concentrations on human keratinocyte cell proliferation and migration were determined using the real time cell analysis system. The scratch assay was performed to determine wound healing activity and supernatants were collected to determine the effects of helenalin on cytokine levels of human keratinocyte cells under inflammatory and non-inflammatory conditions. Under inflammatory conditions, 0.02 and 0.2 μM helenalin increased keratinocyte proliferation and induced the most wound healing and anti-inflammatory activity in human keratinocyte cells. Cell proliferation and migration, wound healing and anti-inflammatory effects were higher in cells exposed to 0.02 μM helenalin than 0.2 μM. Helenalin has been found to decrease the production of inflammatory cytokines, especially under inflammatory conditions and increase wound closure by increasing cell proliferation and migration.

show abstract

Effects of DNMT and HDAC Inhibitors (RG108 and Trichostatin A) on NGF-induced Neurite Outgrowth and Cellular Migration

Cited by 8 publications

References 41 publications

Evaluation of Cell Proliferation and Wound Healing Effects of Vitamin A Palmitate-Loaded PLGA/Chitosan-Coated PLGA Nanoparticles: Preparation, Characterization, Release, and Release Kinetics

Evaluation of Cell Proliferation and Wound Healing Effects of Vitamin A Palmitate-Loaded PLGA/Chitosan-Coated PLGA Nanoparticles: Preparation, Characterization, Release, and Release Kinetics

Neuroprotective effects of some epigenetic modifying drugs’ on Chlamydia pneumoniae-induced neuroinflammation: A novel model

Evaluation of Real Time Cell Proliferation, Anti-Inflammatory and Wound Healing Potential of Helenalin on HaCaT Keratinocytes Treated with Lipopolysaccharide Stimulated Monocytes

Contact Info

Product

Resources

About