Effects of disrupted omega-3 desaturase gene construct on fatty acid composition and expression of four fatty acid biosynthetic genes in transgenic Chlorella vulgaris

Lau, Cher Chien; Loh, Saw Hong; Aziz, Azimon Abdul; San, Thye

doi:10.1016/j.algal.2017.07.011

Cited by 18 publications

(7 citation statements)

References 54 publications

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“…Lipid metabolites play an important role in oleaginous microalgae because the fatty acids that are incorporated into various lipid groups are a major component of the microalgae cell and essentially involved in biochemical processes, building of membrane structures, signalling and storage 1 , 2 . Of the various lipid metabolites, storage lipids, mainly triacylglyceride (TAG), remains an interesting target as its rapid increase can be easily manipulated by culture duration and culture medium manipulation 3 . Microalgae accumulate TAG readily as the component is easy to breakdown thus making it easier for the cell to overcome adverse environmental conditions.…”

Section: Introductionmentioning

confidence: 99%

Lipid accumulation patterns and role of different fatty acid types towards mitigating salinity fluctuations in Chlorella vulgaris

Teh

Loh

Aziz

et al. 2021

Sci Rep

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Mangrove-dwelling microalgae are well adapted to frequent encounters of salinity fluctuations across their various growth phases but are lesser studied. The current study explored the adaptive changes (in terms of biomass, oil content and fatty acid composition) of mangrove-isolated C. vulgaris UMT-M1 cultured under different salinity levels (5, 10, 15, 20, 30 ppt). The highest total oil content was recorded in cultures at 15 ppt salinity (63.5% of dry weight) with uncompromised biomass productivity, thus highlighting the ‘trigger-threshold’ for oil accumulation in C. vulgaris UMT-M1. Subsequently, C. vulgaris UMT-M1 was further assessed across different growth phases under 15 ppt. The various short, medium and long-chain fatty acids (particularly C20:0), coupled with a high level of C18:3n3 PUFA reported at early exponential phase represents their physiological importance during rapid cell growth. Accumulation of C18:1 and C18:2 at stationary growth phase across all salinities was seen as cells accumulating substrate for C18:3n3 should the cells anticipate a move from stationary phase into new growth phase. This study sheds some light on the possibility of ‘triggered’ oil accumulation with uninterrupted growth and the participation of various fatty acid types upon salinity mitigation in a mangrove-dwelling microalgae.

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Section: Introductionmentioning

confidence: 99%

Lipid accumulation patterns and role of different fatty acid types towards mitigating salinity fluctuations in Chlorella vulgaris

Teh

Loh

Aziz

et al. 2021

Sci Rep

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“…Omega-3 desaturase encoded by gene ω-3 FAD plays important role in the conversion of linoleic acid to form trienoic fatty acid known as α-linoleic acid [53]. The expression of ω-3 FAD gene was signi cantly (p 0.05) increased by 1.93-fold and 1.65-fold under N and P-limiting condition after 21 days compare to the control, respectively (Fig.…”

Section: Effect Of Culture Conditions On the Expression Of Rbcl And Accd Genes Of Chlorella Sp T4mentioning

confidence: 92%

“…Omega-3 desaturase gene have been successfully overexpressed to increase the α-linoleic acids content [52,51]. Polyunsaturated fatty acid are major constituents of biological membrane which plays important role in maintaining the membrane uidity and are essential for cell growth at low temperatures [53].…”

Section: Effect Of Culture Conditions On the Expression Of Rbcl And Accd Genes Of Chlorella Sp T4mentioning

confidence: 99%

Lipid Productivity and Biosynthesis Genes Response of Indigenous Chlorella sp. T4 Strain under Different Nitrogen and Phosphorus Load

Gumbi

Kumar

Olaniran

2021

Preprint

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Microalgae can synthesize and accumulate high neutral lipids upon exposure to abiotic stress such as nutrient starvation or limitation. In this study, indigenous microalgae Chlorella sp. T4 was cultivated in nitrogen and phosphorus under both limiting and replete conditions. Growth, lipid yield, fatty acid profiles and biosynthetic gene expression levels were determined to ascertain cell’s response under these conditions. An impaired cell growth was observed under nitrogen limiting condition, evident by the lowest biomass yield (0.58±0.03 g L−1) as revealed by low quantum efficiency of photosystem II (Fv/Fm) value and chlorophyll a content. An increase in lipid content yield was observed under nitrogen and phosphorus limiting conditions as compared to the control. Nutrient limiting conditions produced fatty acid methyl ester that is suitable for biodiesel production compared to the control (BG-11). Gene expression analysis using real time q-PCR for photosynthesis (rbcL) and lipid biosynthesis (accD, KAS-1, ω-6 FAD, ω-3 FAD) genes revealed different expression levels under both limiting and replete conditions. Under nutrient limiting conditions, increase in the expression of accD, KAS-1, ω-6 FAD and ω-3 FAD genes was observed, whereas a decrease in rbcL gene expression level was noted. A significant correlation could be drawn between the expression levels of the biosynthetic genes and growth rate, biomass yield, physiological response, lipid yield and fatty acid composition. These results provide an insight into the physiological response and gene expression level under different nutrient levels, which could be harnessed for future genetic engineering of Chlorella sp. T4 for improved lipid production.

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“…Both UMT-M1 and SE-MC4 used in this research are oleaginous native species isolated from the mangrove areas in Terengganu, Malaysia. UMT-M1 has been intensively studied in our previous research for oil and fatty acid productions under various culture conditions, such as nitrogen starvation [3], phytohormones treatments [[4], [5], [6]], as well as strain improvement through genetic modifications [7,8]. On the other hand, SE-MC4 is a non-model species which has been observed to produce more than 50% (of dry weight) of total oil content in our laboratory.…”

Section: Datamentioning

confidence: 99%

De novo whole genome sequencing data of two mangrove-isolated microalgae from Terengganu coastal waters

et al. 2019

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Interest in harvesting potential benefits from microalgae renders it necessary to have the many ecological niches of a single species to be investigated. This dataset comprises de novo whole genome assembly of two mangrove-isolated microalgae (from division Chlorophyta); Chlorella vulgaris UMT-M1 and Messastrum gracile SE-MC4 from Universiti Malaysia Terengganu, Malaysia. Library runs were carried out with 2 × 150 base paired-ends reads, whereas sequencing was conducted using Illumina Novaseq 2500 platform. Sequencing yielded raw reads amounting to ∼11 Gb in total bases for both species and was further assembled de novo. Genome assembly resulted in a 50.15 Mbp and 60.83 Mbp genome size for UMT-M1 and SE-MC4, respectively. All filtered and assembled genomic data sequences have been submitted to National Centre for Biotechnology Information (NCBI) and can be located at DDBJ/ENA/GenBank under the accession of VJNP00000000 (UMT-M1) and VIYE00000000 (SE-MC4).

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Effects of disrupted omega-3 desaturase gene construct on fatty acid composition and expression of four fatty acid biosynthetic genes in transgenic Chlorella vulgaris

Cited by 18 publications

References 54 publications

Lipid accumulation patterns and role of different fatty acid types towards mitigating salinity fluctuations in Chlorella vulgaris

Lipid accumulation patterns and role of different fatty acid types towards mitigating salinity fluctuations in Chlorella vulgaris

Lipid Productivity and Biosynthesis Genes Response of Indigenous Chlorella sp. T4 Strain under Different Nitrogen and Phosphorus Load

De novo whole genome sequencing data of two mangrove-isolated microalgae from Terengganu coastal waters

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