De novo whole genome sequencing data of two mangrove-isolated microalgae from Terengganu coastal waters

Abstract

Interest in harvesting potential benefits from microalgae renders it necessary to have the many ecological niches of a single species to be investigated. This dataset comprises de novo whole genome assembly of two mangrove-isolated microalgae (from division Chlorophyta); Chlorella vulgaris UMT-M1 and Messastrum gracile SE-MC4 from Universiti Malaysia Terengganu, Malaysia. Library runs were carried out with 2 × 150 base paired-ends reads, whereas sequencing was conducted using Illumina Novaseq 2500 platform. Sequencing yielded raw reads amounting to ∼11 Gb in total bases for both species and was further assembled de novo. Genome assembly resulted in a 50.15 Mbp and 60.83 Mbp genome size for UMT-M1 and SE-MC4, respectively. All filtered and assembled genomic data sequences have been submitted to National Centre for Biotechnology Information (NCBI) and can be located at DDBJ/ENA/GenBank under the accession of VJNP00000000 (UMT-M1) and VIYE00000000 (SE-MC4).

Lipid accumulation patterns and role of different fatty acid types towards mitigating salinity fluctuations in Chlorella vulgaris

“…The observed non-linear growth patterns (Fig. 2 a,b) in response to salinity could point to adaptive flexibility adopted by mangrove-dwelling species like UMT-M1 11 and could have been more beneficial for mangrove habitat dynamics. The results thus affirm that UMT-M1 has euryhaline properties and is capable of easily adapting to different salinities.…”

“…C. vulgaris UMT-M1 11 was obtained from the microalgae culture collection of Universiti Malaysia Terengganu. Inoculum was initiated from a single colony from purified stock agar plate and established in Guillard’s F2 media 42 prepared with 2.5 L of 30 ppt artificial seawater (Instant Ocean Salt, Aquarium Systems).…”

“…Culture condition and salinity treatments. C. vulgaris UMT-M1 11 www.nature.com/scientificreports/ wavelength measurements was recorded at OD 681 using micro plate reader VarioskanTM LUX (Thermo Fisher Scientific, USA). Cell numbers were correlated with OD 681 values to obtain cell calibration curve.…”

“…Mangroves are interesting ecosystems with daily transition gradients between freshwater and marine environments. For this reason, mangrove-dwelling microalgae strains may carry inherent adaptative strategies that may hold the key to overcoming the ‘high yield—low biomass’ conundrum but are unfortunately lesser studied 11 . Hence, experiments carried out at very high salinities (up to 150 parts per thousand (ppt)) may not be able to properly represent physiological aspects of mangrove-dwelling microalgae.…”

Double-high in palmitic and oleic acids accumulation in a non-model green microalga, Messastrum gracile SE-MC4 under nitrate-repletion and -starvation cultivations

“…M. gracile strain SE-MC4, was previously isolated from Setiu Wetland, Terengganu, Malaysia 51 and maintained at the microalgae stock collection in Universiti Malaysia Terengganu. Inoculum culture was initiated from a single colony and subsequently cultivated until Day 12 (early stationary growth phase) in 2.5 L of axenic F2 medium in 3.0 L Erlenmeyer flask.…”