Effects of disinfectants and ciprofloxacin on quorum sensing genes and biofilm of clinical Pseudomonas aeruginosa isolates

Uzunbayir-Akel, Nilufer; Tekintaş, Yamaç; Yılmaz, Fethiye Ferda; Öztürk, İsmail; Okeer, Mustafa; Aydemir, Sabire; Çilli, Fatma Feriha; Hoşgör-Limoncu, Mine

doi:10.1016/j.jiph.2020.10.002

Cited by 13 publications

(8 citation statements)

References 31 publications

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“…QS is one of the principal mechanisms responsible for—among others—the regulation of virulence factor expression and biofilm formation. QS in Pseudomonas consist of a three interconnected systems, namely the Las, Rhl, and Pqs systems; these systems facilitate the changes needed for the pathogens’ survival, by the sensing of diffusible signal molecules found in their surroundings, which is a proxy for the population density in the niche [ 96 , 97 , 98 ]. However, many antibiotics also have the ability to affect these QS systems in Pseudomonas , either by directly acting on gene expression or by the degradation of these signal molecules [ 99 ].…”

Section: Discussion Review Of the Literaturementioning

confidence: 99%

No Correlation between Biofilm Formation, Virulence Factors, and Antibiotic Resistance in Pseudomonas aeruginosa: Results from a Laboratory-Based In Vitro Study

et al. 2021

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Pseudomonas aeruginosa (P. aeruginosa) possesses a plethora of virulence determinants, including the production of biofilm, pigments, exotoxins, proteases, flagella, and secretion systems. The aim of our present study was to establish the relationship between biofilm-forming capacity, the expression of some important virulence factors, and the multidrug-resistant (MDR) phenotype in P. aeruginosa. A total of three hundred and two (n = 302) isolates were included in this study. Antimicrobial susceptibility testing and phenotypic detection of resistance determinants were carried out; based on these results, isolates were grouped into distinct resistotypes and multiple antibiotic resistance (MAR) indices were calculated. The capacity of isolates to produce biofilm was assessed using a crystal violet microtiter-plate based method. Motility (swimming, swarming, and twitching) and pigment-production (pyoverdine and pyocyanin) were also measured. Pearson correlation coefficients (r) were calculated to determine for antimicrobial resistance, biofilm-formation, and expression of other virulence factors. Resistance rates were the highest for ceftazidime (56.95%; n = 172), levofloxacin (54.97%; n = 166), and ciprofloxacin (54.64%; n = 159), while lowest for colistin (1.66%; n = 5); 44.04% (n = 133) of isolates were classified as MDR. 19.87% (n = 60), 20.86% (n = 63) and 59.27% (n = 179) were classified as weak, moderate, and strong biofilm producers, respectively. With the exception of pyocyanin production (0.371 ± 0.193 vs. non-MDR: 0.319 ± 0.191; p = 0.018), MDR and non-MDR isolates did not show significant differences in expression of virulence factors. Additionally, no relevant correlations were seen between the rate of biofilm formation, pigment production, or motility. Data on interplay between the presence and mechanisms of drug resistance with those of biofilm formation and virulence is crucial to address chronic bacterial infections and to provide strategies for their management.

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Section: Discussion Review Of the Literaturementioning

confidence: 99%

No Correlation between Biofilm Formation, Virulence Factors, and Antibiotic Resistance in Pseudomonas aeruginosa: Results from a Laboratory-Based In Vitro Study

et al. 2021

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“…Variability in the sensitivity to antivirulence and/or anti-QS agents among clinical P. aeruginosa isolates has been previously reported [ 53 , 54 ] and needs careful consideration when planning the use of such agents for therapeutic purposes. In a recent study, ciprofloxacin and a number of widely used disinfectants were found to reduce, or, to induce higher expression levels of QS-related genes in different P. aeruginosa strains [ 55 ]. These observations warrant future studies aimed at testing antivirulence agents, used alone or in combination with each other, against large panels of clinical isolates to fully explore the therapeutic potential of virulence-suppressing compounds.…”

Section: Discussionmentioning

confidence: 99%

Antivirulence Properties of a Low-Molecular-Weight Quaternized Chitosan Derivative against Pseudomonas aeruginosa

et al. 2021

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The co-occurrence of increasing rates of resistance to current antibiotics and the paucity of novel antibiotics pose major challenges for the treatment of bacterial infections. In this scenario, treatments targeting bacterial virulence have gained considerable interest as they are expected to exert a weaker selection for resistance than conventional antibiotics. In a previous study, we demonstrated that a low-molecular-weight quaternized chitosan derivative, named QAL, displays antibiofilm activity against the major pathogen Pseudomonas aeruginosa at subinhibitory concentrations. The aim of this study was to investigate whether QAL was able to inhibit the production of relevant virulence factors of P. aeruginosa. When tested in vitro at subinhibiting concentrations (0.31–0.62 mg/mL), QAL markedly reduced the production of pyocyanin, pyoverdin, proteases, and LasA, as well as inhibited the swarming motility of three out of four P. aeruginosa strains tested. Furthermore, quantitative reverse transcription PCR (qRT-PCR) analyses demonstrated that expression of lasI and rhlI, two QS-related genes, was highly downregulated in a representative P. aeruginosa strain. Confocal scanning laser microscopy analysis suggested that FITC-labelled QAL accumulates intracellularly following incubation with P. aeruginosa. In contrast, the reduced production of virulence factors was not evidenced when QAL was used as the main polymeric component of polyelectrolyte-based nanoparticles. Additionally, combination of sub-MIC concentrations of QAL and tobramycin significantly reduced biofilm formation of P. aeruginosa, likely due to a synergistic activity towards planktonic bacteria. Overall, the results obtained demonstrated an antivirulence activity of QAL, possibly due to polymer intracellular localization and QS-inhibition, and its ability to inhibit P. aeruginosa growth synergizing with tobramycin.

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“…Thus, bacteria living in a biofilms matrix can alter their metabolism and modify their gene expression to persist under several stressors or adverse conditions, including the presence of antibiotics (Olsen, 2015; Song et al, 2016), constituting a reservoir for future infections in aquaculture systems (Balcázar et al, 2015; De Carvalho, 2018; Heckman & Soto, 2021). In addition, antibiotics and disinfectants can also induce bacteria to produce biofilms (Ferrer et al, 2017; Kaplan, 2011; Penesyan et al, 2020; Uzunbayir‐Akel et al, 2020). It was recently reported that at subinhibitory concentrations, FLO can increase biofilm formation in A. salmonicida atypical isolates (Desbois et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Subinhibitory concentrations of florfenicol increase the biofilm formation of Piscirickettsia salmonis

Oliver

Céspedes

Santibáñez

et al. 2023

Journal of Fish Diseases

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Piscirickettsia salmonis is a Gram-negative facultative intracellular γ-proteobacterium, classified into two genogroups: LF-89 T (ATCC VR-1361) and EM-90 strains (Nourdin-Galindo et al., 2017). This bacterium is the aetiological agent of piscirickettsiosis, a systemic fish disease characterized by the bacterial colonization of several organs, including liver, kidney, spleen, skeletal muscle, intestine and gills among others (Rozas & Enríquez, 2014), causing severe economic losses in Chilean salmonid farms (Sernapesca, 2022a). Although the main disease outbreaks and the subsequent associated mortality had been identified in southern Chile, this disease has also been reported in several countries (Rozas & Enríquez, 2014). The Chilean salmon industry uses several biosecurity measures as a way to mitigate the impact of infectious diseases. Thus, 29 different vaccine formulations are currently approved for use against piscirickettsiosis (SAG, 2022) Statistical data from The Chilean National Fisheries and Aquaculture Service (SERNAPESCA) indicated that piscirickettsiosis is the most prevalent bacterial disease affecting Chilean salmon (Sernapesca, 2022a). For this reason, antibiotics remain the primary treatment against piscirickettsiosis outbreaks. The Chilean salmon industry used 4634 tons of antibiotics in

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Effects of disinfectants and ciprofloxacin on quorum sensing genes and biofilm of clinical Pseudomonas aeruginosa isolates

Cited by 13 publications

References 31 publications

No Correlation between Biofilm Formation, Virulence Factors, and Antibiotic Resistance in Pseudomonas aeruginosa: Results from a Laboratory-Based In Vitro Study

No Correlation between Biofilm Formation, Virulence Factors, and Antibiotic Resistance in Pseudomonas aeruginosa: Results from a Laboratory-Based In Vitro Study

Antivirulence Properties of a Low-Molecular-Weight Quaternized Chitosan Derivative against Pseudomonas aeruginosa

Subinhibitory concentrations of florfenicol increase the biofilm formation of Piscirickettsia salmonis

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