Effects of dimethylsulfoxide and pentoxifylline in the vitality of cutaneous flaps in rats

Abstract

A B S T R A C T

Benefits of pentoxifylline for skin flap tissue repair in rats

“…Pentoxifylline has a classical therapeutic indication for the treatment of circulatory disorders and occlusive peripheral arterial diseases, studies have administration included a protective effect against flap necrosis, when administered orally and parenterally. Even though, administration of pentoxifylline by subcutaneous route has minimal systemic repercussions, over the oral route, and it does not require repeated doses to reach the desired serum level 3,9 . And provides effective fluid absorption, adequately uniform and slow dispersion, concentration at the site where the action is expected to occur, and easy application 3,9,23,24 .…”

“…Total flap, necrotic tissue and viable tissue area were measured, in vivo, with a digital caliper, and the macroscopic features of the flap on the tenth day (D10) after the operation were recorded and reported to the database for statistical analysis 9,26 . The photographic documentation occurred on days D1, D3, D5, D7 and D10, together with clinical observation of the flaps and all research animals by the same researcher during the experiment stages with a digital camera Cannon EF 100 mm, USM Tokyo, Japan.…”

“…For the morphometric analysis of the histological elements, images of sequential sections of each skin segment were obtained: 25 fields (514.764 µm 2 ) for the number of vessels, 10 fields (30.815 µm 2 ) for epithelial thickness, and 20 fields (137.910 µm 2 ) for collagen density 27 . The analysis, was made from digitized images obtained from the histological fields in a light microscope with a ×40 plan achromatic objective 9,14,27 .…”

“…In several experimental studies 3,[6][7][8][9] , drugs and other therapeutic modalities were used to mitigate the freeradical formation process, stimulate tissue repair and decrease tissue edema. Among these drugs, pentoxifylline has hemorheological properties 10 .…”

“…It plays a role in morphological immunomodulation, decreases the inflammatory action, and has a fibrinogenic action, with decreased collagen synthesis. This potent antioxidant acts by inhibiting the superoxide anion synthesis and may reduce the toxic reperfusion metabolites, with a reduction in those responsible for tissue damage and consequent increase in flap survival 8,9,[11][12][13][14][15][16][17][18] .…”