Effects of Different Zinc Sources on Performance, Bio Distribution of Minerals and Expression of Genes Related to Metabolism of Broiler Chickens

Abstract

Different sources of Zinc (Zn) were compared to assess their possible effects on performance, nutrients retention, mineral distribution and some serum parameters of broiler chickens. A total of 200 one-day old Ross 308 chicks were divided into in equal four dietary treatments groups with five replicates each of ten chicks. The experimental groups were given the basal diet (inorganic ZnO), basal diet supplemented with organic Zn (Zn methionine), nano-ZnO and Znmix (organic Zn and nano-ZnO) at a concentration of 50 mg/kg of diet. After 42 days of feeding trial, the group supplemented with nano-ZnO exhibited the best final body weight and feed conversion ratio (2380 g/bird and 1.69, respectively). Nano-ZnO and Zn-mix supplementation significantly increased crude fat retention (86.70 and 86.75%, respectively). All sources of supplemented Zn other than inorganic ZnO significantly increased (P<0.05) Zn retention especially in the group supplemented with nano-ZnO (41.8%). Organic Zn and/or nano-ZnO sources supplemented to broiler diets significantly increased (P<0.05) iron and copper contents in the hepatic tissue and Zn content in the tibia. The mean of serum total cholesterol, triglycerides and very low density lipoprotien were significantly reduced (P<0.05) by dietary supplementation of organic Zn and/or nano-ZnO. The activity of malondialdehyde was significantly decreased (P<0.05), while Cu/Zn superoxide dismutase activity was significantly increased (P<0.05) by addition nano-ZnO or Zn-mix. Dietary Zn-mix and nano-ZnO positively affected mRNA expression of insulin like growth factor-1 and growth hormone genes in broilers when compared to the inorganic ZnO source. The present findings prospected that replacing traditional inorganic ZnO source with nano-ZnO or combining nano-ZnO and Zn methionine at applied concentration, promoted the growth of broilers, enhanced Zn up take and antioxidant status without negative effect on selected minerals distribution in tissues.

Efficacy of Dietary Zinc Oxide Nanoparticles Supplementation on Serum Biochemical, Nutrients Retention and Chemical Composition of Meat and Tibia in Broiler Chickens

“…The increase in the crude protein and ash of meat, as well as the minerals (Ca, P and Zn) in meat and tibia, is due to increasing the same nutrients in blood serum and to improving their digestibility. The results of the current study agree with those reported by Ibrahim et al (2017) who showed that the highest tibia Zn content was recorded with diets supplemented with N-ZnO compared with that supplemented with I-ZnO of broiler chicks. Similarly, tibia Zn content was significantly higher with the addition of 80 mg N-Zn/kg to broiler diet in comparison with 80 mg Zn-sulphate or the unsupplemented control (Mohammadi et al, 2015).…”

“…In addition, N-Zn decreased (P≤0.05) serum total cholesterol, VLDL and triglyceride, in broiler chicks with the lowest values were observed with N-Zn diets. In spite of that, N-Zn supplementation had no effect on serum lipid and HDL (Ibrahim et al, 2017). Similar results have been obtained by Ahmadi et al(2013) who reported that supplementation of 60 or 90 mg N-ZnO/kg of broiler diet decreased serum triglycerides, total cholesterol and LDLcholesterol with no significant differences, however, HDL-cholesterol increased (P <0.05) in comparison with the control diet (zero N-ZnO).…”

“…Birds fed 40 mg N-ZnO exhibited the highest N, Ca, P and Zn retention percentages compared with those fed the control which recorded the lowest percentages. In harmony with the current data, Ibrahim et al (2017) on broilers and Tsai et al (2016) on laying hens concluded that Zn retention was significantly increased by N-ZnO supplementation compared with I-ZnO because of its higher bioavailability. Also, Yan and Waldroup (2006) reported that Zn excretion could be reduced in the excreta by the supplementation of N-Zn to poultry diets.…”

“…These data are in harmony with those obtained by Berg and Shi (1996) and Walsh et al (1994) who stated inverse relationship between N-ZnO and MDA values and serum ALT and AST enzyme concentrations. Besides, Ibrahim et al (2017) reported that serum MDA level was decreased (P≤0.05), while, the Cu/Zn-SOD level was increased (P≤0.05) with dietary N-ZnO, which indicated that adding of N-Zn increased the antioxidant activity in broilers. However, the hepatic activity of AST and ALT were not significantly influenced by different dietary sources of Zn.…”

Nanotechnology and its Considerations in Poultry Field: An Overview

“…In the study of Hassan et al (2013), it was found that feed treatments with different concentrations of ZnO nano particles inhibited the growth of mycotoxic fungi (A. flavus, A. ochraceus and A. niger) and consequently mycotoxicosis conditions. Ibrahim et al (2017) suggested that replacing traditional inorganic ZnO source with nano-ZnO or combining nano-ZnO and Zn methionine at applied concentration, promoted the growth of broilers, enhanced Zn uptake and antioxidant status without negative influence on selected minerals distribution in broilers tissues. In addition, compared to inorganic-Zn form, supplementation and/or substitution with organic-Zn and/or nano-Zn form (20 ppm) had a positive influence on broilers body weight, body weight gain and feed conversion rate, Zn concentration in bird's serum and tissue, and increased return and net profit (Badawi et al, 2017).…”