Effects of different preservation solutions on skin graft epidermal cell viability and graft performance in a rat model

Başaran, Ö.; Ozdemır, B. Handan; Kut, Altuğ; Şahin, Feride İffet; Deniz, Murat; Sakallıoğlu, Ebru Ayşe; Haberal, Mehmet

doi:10.1016/j.burns.2005.11.010

Cited by 21 publications

(13 citation statements)

References 19 publications

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“…Basaran et al found RPMI superior to other media [21]. They explained this superiority by the rich amino acid content of RPMI, which helps to improve preservation of cells.…”

Section: Discussionmentioning

confidence: 95%

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Histological assessment of skin grafts stored in amniotic fluid and saline

Turhan-Haktanir

Şahin

Yagmurca³

et al. 2010

Journal of Plastic Surgery and Hand Surgery

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We investigated the efficacy of amniotic fluid as a substance in which to store grafts; it is rich in nutrients, proteins, and growth factors, and has well-known antimicrobial features. We compared it with the widely-used and practical saline. Split-thickness grafts 4 × 4 cm were prepared from the back of 20 rats and divided into four groups (n = 5 each). The rolled grafts were wrapped in gauze dampened with saline or amniotic fluid and placed into refrigerators in sterile containers for storage. On days 7, 14, 21, and 28, histological examinations were made. A semiquantitative evaluation of the histological damage to the skin was made by scoring its degree of severity. Compared with saline, histological scores in the grafts stored in amniotic fluid were found to be significantly lower on the 14th, 21st, and 28th days (p values on days 14, 21, and 28; cell swelling: 0.014, 0.006, and 0.005, respectively; nuclear swelling: 0.003, 0.006, and 0.007, respectively; nuclear pleomorphism: 0.004, 0.005, and 0.003, respectively; nuclear haloes: 0.015, 0.005, and 0.005, respectively; nuclear pyknosis: 0.003, 0.005, and 0.003, respectively; dermo-epidermal clefting: 0.005, 0.003, and 0.003, respectively; eosinophilia and mitosis: 0.003, 0.006, and 0.004, respectively; dermal collagen: 0.003, 0.003, and 0.003, respectively). Amniotic fluid maintained preservation better for skin grafts than saline. Comparison with other modern storage media would be beneficial.

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“…Basaran et al found RPMI superior to other media [21]. They explained this superiority by the rich amino acid content of RPMI, which helps to improve preservation of cells.…”

Section: Discussionmentioning

confidence: 95%

“…Although storage at 4 C after the graft has been wrapped in gauze soaked in physiological saline is an inferior technique [21], it is still widely used by clinicians because of practicality and the expense of the other techniques [8,11,17].…”

Section: Discussionmentioning

confidence: 99%

Histological assessment of skin grafts stored in amniotic fluid and saline

Turhan-Haktanir

Şahin

Yagmurca³

et al. 2010

Journal of Plastic Surgery and Hand Surgery

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“…The trypan blue dye exclusion test was used, as previously described. 2,13 The skin was rinsed in normal saline, minced with surgical scissors into 1-mm pieces, and transferred to a Petri dish containing a trypsin solution for 30 minutes of enzymatic treatment at 37-C to separate dermal and epidermal layers (Fig. 1).…”

Section: Keratinocyte Survivalmentioning

confidence: 99%

Amniotic Fluid for Ex Vivo Skin Preservation

Buseman

Rinker

2013

Annals of Plastic Surgery

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Ex vivo skin preservation is important for skin banks, burn centers, and in research; however, the optimal preservation solution is not known. Human amniotic fluid (HAF), in addition to its role in fetal wound healing, has promise as an effective and readily available preservation solution. The purpose of this study was to compare the efficacy of several solutions, including HAF, in full-thickness skin preservation. Human amniotic fluid was obtained from patients undergoing amniocentesis. Full-thickness skin obtained during abdominoplasty was divided into 1-cm(2) samples. These specimens were preserved in either saline, HAF from a single patient, pooled HAF, University of Wisconsin solution, or custodial histidine-tryptophan-ketoglutarate solution at 4°C. There were 5 samples in each group. Specimens were examined for keratinocyte survival at 7, 14, 21, 28, and 35 days using the trypan blue assay. The first 200 cells identified were counted to calculate the degree of cell death. Comparisons were made between the groups, and a multivariable repeated-measures analysis was performed to determine statistical significance, which was defined as P < 0.05. The individual and pooled HAF showed greater keratinocyte survival than the other solutions, at each time point. The difference was statistically significant (P < 0.05) when compared to saline and custodial solution but not statistically significant when compared to University of Wisconsin solution. When used as a tissue preservation solution, HAF produces superior keratinocyte survival than several commercially available solutions. Amniotic fluid, nature's tissue preservation solution, may be an inexpensive, readily available alternative for use in skin banks, burn centers, and research.

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“…However, the availability of fresh allograft do not coincide with occurrence of severe burns and the shelf life of fresh allograft at normal refrigeration temperature is limited (DeBono et al 1998), thus skin graft preservation for the purpose of delayed application has become a vital tool in burn therapeutics. Preserved allograft allows for a longer storage period and allows more time for the sample to be assessed microbiologically to certify its sterility and viability (Bravo et al 2000;Castagnoli et al 2003;Başaran et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Comparing the use of glycerol preserved and cryopreserved allogenic skin for the treatment of severe burns: differences in clinical outcomes and in vitro tissue viability

et al. 2011

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Cryopreserved (CryoPA) and Glycerol-preserved (GPA) skin allografts are commonly used in the treatment of severe burn injuries. However, comparable data on their differences in clinical outcome is scarce. This retrospective review aims to study the effect of allograft viability on clinical outcomes. The records of 48 severe burn patients who either received CryoPA or GPA were reviewed. Key burn mortality determinants were used to match the 2 groups. Clinical outcomes such as mortality rate (MR) and the length of hospital stay (LOS) were obtained. A separate in vitro comparison included histological assessments and the use of tetrazolium reductase activity to compare tissue viability. Both groups showed a comparable profile in burn mortality determinants. Patients who received CryoPA had a lower MR of 25% compared to 34.8% (P=0.250) in the GPA group and a lower LOS of 39.2-45.9 days (P=0.730), respectively. The histological structural integrity was found to be well preserved with both methods although CryoPA was confirmed to be the more viable product (P<0.05). The lower MR associated with CryoPA cannot be totally ignored. However, the mechanism through which viable skin allografts improves MR of severe burns patients remains to be elucidated.

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Effects of different preservation solutions on skin graft epidermal cell viability and graft performance in a rat model

Cited by 21 publications

References 19 publications

Histological assessment of skin grafts stored in amniotic fluid and saline

Histological assessment of skin grafts stored in amniotic fluid and saline

Amniotic Fluid for Ex Vivo Skin Preservation

Comparing the use of glycerol preserved and cryopreserved allogenic skin for the treatment of severe burns: differences in clinical outcomes and in vitro tissue viability

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