Effects of different levels of modified palygorskite supplementation on the growth performance, immunity, oxidative status and intestinal integrity and barrier function of broilers

Abstract

This experiment was designed to investigate effects of different levels of modified palygorskite (MPal) supplementation on growth performance, immunity, oxidative status and intestinal integrity and barrier function of broilers. A total of 320 1-day-old Arbor Acres broilers were randomly assigned into 5 dietary treatments and fed a basal diet supplemented with 0, 0.25, 0.5, 1 and 2 g/kg MPal, respectively, for a 42-day feeding trial. Treatments quadratically reduced feed/gain ratio (F:G) during 1-21 days and linearly decreased average daily feed intake and F:G during 22-42 days, and linearly and quadratically decreased average daily feed intake and F:G during overall period (p < 0.05, 0.50 g/kg treatment showed the lowest F:G). MPal supplementation increased the contents of 21-day jejunal secretory immunoglobulin A (SIgA) quadratically, and 21-day jejunal immunoglobulin G (IgG), immunoglobulin M (IgM) and 42-day jejunal total superoxide dismutase (T-SOD) activity linearly and quadratically (0.50 g/kg treatment showed the highest immunoglobulin concentration), whereas linearly reduced 21-day ileal SIgA level and 42-day jejunal malondialdehyde (MDA) accumulation and serum diamine oxidase activity, and quadratically decreased 21-day ileal MDA level (p < 0.05). The 42-day jejunal SIgA, IgG and IgM concentrations, and T-SOD activity in jejunum at 21 days and ileum at both 21 days and 42 days were quadratically increased with MPal administration (p < 0.05, 0.50 g/kg treatment showed the highest T-SOD activity). The mucin 2 mRNA abundances in 42-day jejunum and 21-day ileum were quadratically increased with MPal supplementation (p < 0.05). Treatments linearly increased 42-day ileal zonula occludens-1, claudin-3 and jejunal claudin-3 mRNA level, whereas linearly and quadratically increased ileal claudin-2 mRNA level (p < 0.05). In conclusion, MPal supplementation exhibited beneficial effects on growth performance, intestinal immunity, antioxidant capacity and intestinal integrity and barrier function of broiler with its optimum dosage being 0.5 g/kg.

Modified Palygorskite Improves Immunity, Antioxidant Ability, Intestinal Morphology, and Barrier Function in Broiler Chickens Fed Naturally Contaminated Diet with Permitted Feed Concentrations of Fusarium Mycotoxins

“…In the current study, we observed that dietary MPal supplementation increased relative bursa of Fabricius weight and SIgA concentration in the jejunal and ileal mucosa at 42 days, and it was in agreement with the results by Yin et al [ 48 ] and Weaver et al [ 50 ]. Researches from our lab have illustrated that this MPal exerted better adsorption capacity to zearalenone [ 39 ], and improved immunity, intestinal antioxidant status, as well as barrier integrity of animals [ 37 , 38 ], which would, partially and eventually, result in better immune function of broiler chickens challenged with permitted feed concentrations of Fusarium mycotoxins in the present study.…”

“…In broiler chickens, Chen et al have reported that dietary Pal supplementation could improve immunity via enhancing intestinal IgM and SIgA contents [ 34 ]. Similarly, improved immunity of broiler chickens was observed by appropriate levels of MPal addition in a recent investigation, as evidenced by increased IgM, IgG, and SIgA contents in the intestinal mucosa at 42 days [ 37 ]. Moreover, it has been proven that a modified clay (montmorillonite) could elevate serum IgG concentration in laying hens [ 49 ].…”

“…In broiler chickens, Chen et al have reported that Pal supplementation into diet could increase intestinal SOD activity [ 34 ]. Moreover, our laboratory members have currently demonstrated that MPal aforementioned could improve intestinal antioxidant status in broiler chickens and laying hens, as evidenced by decreased MDA concentration while increasing antioxidant enzymes including SOD and total-antioxidant capacity [ 37 , 38 ]. In the present study, we observed that this MPal reduced jejunal MDA content at both 21 and 42 days and jejunal SOD activity at 42 days, whereas increased ileal SOD activity at 42 days when compared with the mycotoxins group.…”

“…It is accordingly necessary that Pal needs to be modified (modified Pal, MPal) to improve its adsorption capacity of nonpolar mycotoxins. Recent studies in our lab have shown that Pal modified with an organic cation surfactant could improve intestinal oxidative status, immunity, and/or barrier integrity in laying hens and broiler chickens fed with a normal diet [ 37 , 38 ]. Moreover, this MPal exerted a better adsorptive capacity to zearalenone [ 39 ] and also efficiently reduced hepatic zearalenone residue as well as relieved hepatic oxidative damage in broiler chickens given a diet contaminated with zearalenone [ 40 ].…”

Effects of berberine on the growth performance, antioxidative capacity and immune response to lipopolysaccharide challenge in broilers

“…This finding was also consistent with that of Shen et al (). Immunoglobulins are an important component of the humoural immune system, which regulates the host's defensive immune response by different strategies, such as neutralizing viruses, phagocytosing antigen and antibacterial effects (Su et al, ). In this study, a significant increase in IgM and IgG levels was observed after LPS injection, and it was similar to the previous results of Kariyawasam Wilkie Hunter and Gyles (), who reported that LPS could induce the production of immunoglobulins and participate in the inflammatory response in broilers.…”