Effects of different dietary phytase activities on the concentration of antioxidants in the liver of growing broilers

Abstract

One-hundred and fifty male chickens were used to evaluate the effects of different activities (0, 250, 500, 12 500 FTU/kg) of phytase on their performance and antioxidant concentration in the liver. The chicks were housed in 30 cages and were allocated to six replicates of five dietary treatments. All diets were formulated to be adequate in energy and protein (12.90 MJ/kg metabolizable energy, 214 g/kg crude protein), however, the negative control (NC) was lower in available P compared with the positive control (PC) (2.5 vs. 4.5 g/kg diet). The other three diets were the NC supplemented with phytase at 250, 500 and 12 500 FTU/kg (NC + 250, NC + 500 and NC + 12 500 FTU respectively). The concentration of antioxidants in the liver of the birds was determined using HPLC at 21 days of age. Low P diets (NC) reduced weight gain, however, supplementation with phytase improved weight gain to the extent that it was better than the PC at the 12 500 FTU treatment (p < 0.05). Feed conversion ratio was also improved by the high level of phytase supplement more than other treatments (p < 0.05). Feed consumption was not affected either by dietary phosphorus concentration or by different phytase supplementation. The antioxidant data showed that the unsupplemented diet with low phosphorus (NC) decreased the concentration of coenzyme Q(10) and retinol-linoleate in the liver compared with that of birds on the adequate phosphorus treatment (PC). Phytase supplementation, especially at the higher doses (500 and 12 500 FTU) increased the level of coenzyme Q(10) to the same level as the PC treatment. In addition, the highest dose (12 500 FTU) of phytase increased retinol concentration in the liver of chickens compared with those on the NC treatment. The highest inclusion level of phytase increased the alpha-tocopherol level in the liver compared with the lower levels of phytase (NC + 250 and NC + 500 FTU).

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“…and it is less likely to be an immediate participant in metabolic process. In a recent study, synthesis of myo-inostiol by phytase addition was inferred by Karadas et al (2010) where the addition of 12,500 FTU/kg of a bacterial phytase resulted in increases in vitamin E and A and coenzyme Qm in the liver of chickens. Few contemporary articles have presented the effect of myoinostiol on the performance, physiology, or nutrition of poultry or swine.…”

Effect of inositol and phytases on hematological indices and α-1 acid glycoprotein levels in laying hens fed phosphorus-deficient corn-soybean meal-based diets

“…Phytase B alone, and particularly in combination with phytase A, acted as a potent mediator of the response, whereas supplementary myo-inositol did not. rimental effects of phytate on intestinal amylase, disaccharidases, and Na + K + -ATPase activities, as well as contributes to the elevation of hepatic carotenoid, α-tocopherol, retinol, and coenzyme Q 10 concentrations (Liu et al, 2008;Karadas et al, 2010). Some results of our study (Żyła et al, 2012) demonstrated that phytase B alone, or in combination with 6-phytase A, reduced the total lipid and cholesterol concentrations in egg yolks collected from the hens fed the corn-soybean meal-based (CSM) diets; furthermore, the combination of both phytases also improved the n-6:n-3 polyunsaturated fatty acids (PUFA) ratio.…”

Dietary xylanase increases hepatic vitamin E concentration of chickens fed wheat based diet

“…The concentration of hepatic vitamin E and carotenoids was determined as described by Karadas et al (2006Karadas et al ( , 2010 using high-performance liquid chromatography system (Shimadzu Liquid Chromatograph, LC-10AD, Japan Spectroscopic Co. Ltd.). …”