Effect of inositol and phytases on hematological indices and α-1 acid glycoprotein levels in laying hens fed phosphorus-deficient corn-soybean meal-based diets

Abstract

The effects of feeding low nonphytate phosphorus (NPP) corn-soybean meal-based diets supplemented with myo-inositol at 0.1%, or with phytase B at 1,300 acid phosphatase units/kg, or with phytase B enriched in 6-phytase A at 300 phytase units/kg on the hematological indices and the α-1 acid glycoprotein (AGP) concentrations in the blood of Bovans Brown laying hens were investigated. The experimental design comprised also a negative control diet and an internal control diet that had the NPP content adjusted by the addition of 0.304 g of monocalcium phosphate per kg to reach the NPP level similar to that resulting from the combined action of both phytases. A total of sixty 50-wk-old hens were randomly assigned to the dietary treatments with 12 cage replicates of 1 hen, and fed the experimental diets until wk 62, when the blood samples were taken and analyzed for basic hematological indices and for AGP concentrations in sera. The hematological indices from all the experimental groups remained in a normal range; nevertheless, the statistically significant effects of diet on hemoglobin concentration (P = 0.003), erythrocyte counts (P = 0.035), the percentage of lymphocytes (P = 0.020), heterophils (P = 0.002), eosinophils (P = 0.023), and basophils (P = 0.001) in the leucocyte population, as well as on the heterophil to lymphocyte ratio (P = 0.003), were observed. The highest erythrocyte counts were characteristic for hens fed the diet supplemented with both phytase A and phytase B. The highest heterophil to lymphocyte ratios were found in blood of hens fed the diet supplemented with phytase B, whereas the highest basophil percentages and the highest AGP concentrations occurred in birds fed the negative control diet. A highly significant correlation was observed between AGP concentrations in sera and BW losses determined previously. The results indicate that the low-NPP corn soybean meal-based diets increased acute phase protein level in laying hens. Phytase B alone, and particularly in combination with phytase A, acted as a potent mediator of the response, whereas supplementary myo-inositol did not.

Effect of Royal Jelly on behavioural patterns, feather quality, egg quality and some haematological parameters in laying hens at the late stage of production

“…This suggests that laying hens in the RJ‐treated groups had improved welfare status. In accordance with these findings, previous literatures confirmed an improved H/L ratio in old laying hens, and usually related to some feed additives that have antifree radical and antioxidant characteristics (Żyla, Grabacka, Pierzchalska, Duliński, & Starzyńska, ). Hosseini, Vakili Azghandi, Ahani, and Nourmohammadi () recorded that H:L ratio was lower ( p < .05) in chickens received dietary supplements with beehive products compared to the control group.…”

The Impact of Phytases on the Release of Bioactive Inositols, the Profile of Inositol Phosphates, and the Release of Selected Minerals in the Technology of Buckwheat Beer Production

“…This suggests that the cooperation of both phytase A and B, but not phytase A alone, may be efficacious in effecting MI release from buckwheat malt during mashing. In different applications, but mainly in poultry nutrition, the simultaneous supplementation of phytase A and B was found to be superior to single phytase A [31]. On the other hand, it should be noted that despite high dosages of phosphorolytic enzymes (Finase AP at 10 U/g of malt), the pool of the released bioactive MI increased only to around 20% of its concentration in the buckwheat malt.…”

Royal Jelly Improves the Morphology of the Reproductive Tract, Internal Egg Quality, and Blood Biochemical Parameters in Laying Hens at the Late Stage of Production

“…Meanwhile, others reported that propolis supplements had no effect on the total leucocyte counts in laying hens [43] or mice [44]. In the present study, RJ decreased the heterophil percentage and accordingly the H/L ratio, which may indicate an improved welfare condition of treated hens.Żyla et al [45] also indicated that antioxidants and anti-free-radical supplements could improve the H/L ratio in aged laying hens. However, Çetin et al [43] reported that propolis did not alter the differential ratios of leukocytes in laying birds.…”