Effects of different cytokinins on the shoot regeneration from apple leaves of 'Royal Gala' and 'M.26'

Dobránszky, J.; Hudák, I.; Magyar-Tábori, K.; Jámbor-Benczúr, E.; Galli, Zsolt; Kiss, E.

doi:10.31421/ijhs/10/1/442

Cited by 4 publications

(12 citation statements)

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“…However, the predominant type of cytokinin depended on genotype (Kamboj et al 1999). Furthermore, changes induced by cytokinins were also described both at the tissue and cell level (Dobránszki et al 2004). Pre-treatment of apple shoots in liquid medium with TDZ prior to regeneration also had a wide range of effects on leaf cells (Sriskandarajah and Goodwin 1998), and the structural characteristics of leaves altered by cytokinins are related to the organogenic ability of explants .…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 96%

“…The classical cytokinins, namely N 9 -ribosides (applied at lower concentrations of 17-23 lM than nonribosides at 22-30 lM), showed the same or better regeneration, except for BAR. The OI was highest when TOPR was applied at 21.44 lM for 'M26' (Dobránszki et al 2004). The developmental pattern of shoot regeneration of 'M26' under different cytokinins is presented in Fig.…”

Section: Cytokinins During the Regeneration Processmentioning

confidence: 96%

“…Benková et al (1999) described that a substantial part of leaf cytokinins are localized in the chloroplasts. The first evidence for the role of chloroplast development and/or chloroplast gene expression in the response of leaf cells to Dobránszki et al (2004) cytokinin was published by Kulaeva et al (2002), and it was concluded that unimpaired chloroplast development and chloroplast gene expression is required for normal leaf responses to cytokinin. Dobránszki et al (2005) studied the effects of different aromatic cytokinins on the histology of in vitro apple leaves and their post-effects on subsequent shoot regeneration from these leaves.…”

Section: Effects Of Cytokinins On Leaf Anatomy and Subsequent Shoot Rmentioning

confidence: 99%

“…However, very little information is available about its effect on apple organogenesis (Dobránszki et al 2002;Hudák et al 2003Hudák et al , 2005. In some experiments, cytokinin ribosides (kinetin riboside, KINR; zeatin riboside, ZEAR; meta-topolin ribosid, TOPR) were also tested, ZEAR and TOPR can be highly active but not KINR in apple shoot organogenesis (Dobránszki et al 2004(Dobránszki et al , 2006.…”

Section: Cytokinin and Its Role In Shoot Regenerationmentioning

confidence: 99%

“…Similarly, KIN and ZEA applied alone did not induce adventitious shoot formation in 'Fuji' (Ling et al 2002). Dobránszki et al (2004) tested the effects of several cytokinins in the regeneration media by the scheme presented in Fig. 1 (Experiment 2).…”

Section: Cytokinins During the Regeneration Processmentioning

confidence: 99%

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The role of cytokinins in shoot organogenesis in apple

Magyar-Tábori

Dobránszki

Silva

et al. 2010

Plant Cell Tiss Organ Cult

131

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Effective regeneration in vitro is a necessary precondition for the implementation of different biotechnological approaches in plant breeding. Numerous studies have reported on regeneration from apple somatic tissues, and organogenesis has been proved to be influenced by several factors including mother shoots (genotype, size, type, and age of explant), in vitro conditions (dark period, light intensity, and quality), and others (wounding, orientation of leaf explants). However, one of the most important factors before and during the regeneration process is the type and concentration of cytokinin applied. Thidiazuron and benzyladenine are the most frequently used cytokinins in the regeneration systems, but their efficiency depends on genotype and other factors. Other cytokinins (e.g., zeatin and kinetin) have also been tested in several experiments and they were found in general to be less active. The organogenic ability of explants can also be increased by a properly selected cytokinin pre-treatment. Cytokinins applied in the pre-treatments can influence the leaf structure, which in turn can alter the regeneration capacity of the leaf explant. Interactions between factors of pre-treatments (hormones, light, and culture conditions) and factors of the regeneration phase should be considered. This review brings into focus the role of different cytokinins during in vitro shoot development, discussing their effects on the histology of leaves developed in vitro, and how this affects the subsequent regeneration process.

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Section: Conclusion and Future Perspectivesmentioning

confidence: 96%

Section: Cytokinins During the Regeneration Processmentioning

confidence: 96%

Section: Effects Of Cytokinins On Leaf Anatomy and Subsequent Shoot Rmentioning

confidence: 99%

Section: Cytokinin and Its Role In Shoot Regenerationmentioning

confidence: 99%

Section: Cytokinins During the Regeneration Processmentioning

confidence: 99%

See 3 more Smart Citations

The role of cytokinins in shoot organogenesis in apple

Magyar-Tábori

Dobránszki

Silva

et al. 2010

Plant Cell Tiss Organ Cult

131

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In vitro tissue culture of apple and other Malus species: recent advances and applications

Silva

Gulyás

Magyar-Tábori

et al. 2019

Planta

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Main conclusion Studies on the tissue culture of apple have allowed for molecular, biotechnological and applied breeding research to advance. In the past 8 years, over 100 papers advancing basic biology, genetic transformation and cryobiology have emerged. Apple (Malus × domestica Borkh.; Rosaceae) is an important fruit crop grown mainly in temperate regions of the world. In vitro tissue culture is a biotechnological technique that has been used to genetically improve cultivars (scions) and rootstocks. This updated review presents a synthesis of findings related to the tissue culture of apple and other Malus spp. between 2010 and 2018. Increasingly complex molecular studies that are examining the apple genome, for example, in a bid to identify the cause of epigenetic mutations and the role of transposable elements in this process would benefit from genetically stable source material, which can be produced in vitro. Several notable or curious in vitro culture methods have been reported to improve shoot regeneration and induce the production of tetraploids in apple cultivars and rootstocks. Existing studies have revealed the molecular mechanism underlying the inhibition of adventitious roots by cytokinin. The use of the plant growth correction factor allows hypothetical shoot production from leaf-derived thin cell layers relative to conventional leaf explants to be determined. This updated review will allow novices and established researchers to advance apple and Malus biotechnology and breeding programs.

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Topolins: A panacea to plant tissue culture challenges?

Aremu

Bairu

Doležal

et al. 2011

Plant Cell Tiss Organ Cult

160

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Since the discovery of topolins as naturally occurring aromatic cytokinins (CKs), they have emerged as genuine alternatives to the long serving CKs such as benzyladenine, zeatin and kinetin in plant tissue culture (PTC). Globally, the past 15 years has witnessed a surge in the use of topolins and their derivatives in research laboratories. Topolins, especially the meta-topolin and its derivatives have been employed for culture initiation, protocol optimization and for counteracting various in vitro induced physiological disorders in many species. Evidence from various studies indicate the rising popularity and advantages (although not universal for all species) of topolins compared to other CKs. In this review, we assess the use of topolins in PTC with emphasis on their metabolism, structure-activity relations and effect on morphogenesis in vitro. In addition, the review provides a detailed list of species that have been used to study the effect of topolins in comparison with other CKs, the growth parameters affected and recommended concentrations are also provided.

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Effects of different cytokinins on the shoot regeneration from apple leaves of 'Royal Gala' and 'M.26'

Cited by 4 publications

References 0 publications

The role of cytokinins in shoot organogenesis in apple

The role of cytokinins in shoot organogenesis in apple

In vitro tissue culture of apple and other Malus species: recent advances and applications

Topolins: A panacea to plant tissue culture challenges?

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