Effects of different concentrations of benzylaminopurine on shoot regeneration and hypericin content in<i>Hypericum triquetrifolium</i>Turra.

Karakaş, Özgür; Toker, Zuhal; Tilkat, Engin; Özen, Hasan Çetin; Onay, Ahmet

doi:10.1080/14786410701664528

Cited by 13 publications

(3 citation statements)

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“…In H. perforatum and H. adenotrichum , the elevated production of hypericins was achieved by the addition of polyethylene glycol (PEG) or sucrose to culture media ( Pavlík et al., 2007 ; Yamaner and Erdag, 2013 ), and more than 3-fold increase of hypericins was reported in nanoperlite-treated H. perforatum shoot cultures ( Jafarirad et al., 2021 ). In reaction to phytohormones, the accumulation of naphthodianthrones depended on the type, combination, and concentration of phytohormones including the adenine-type (BAP, 6-benzylaminopurine; Kin, kinetin; ZT, zeatin), or phenylurea-type (TDZ; thidiazuron) cytokinins ( Liu et al., 2007 ; Karakas et al., 2009 ; Coste et al., 2011 ). The hypericins content also increased when the stress-signaling molecules salicylic acid (SA), jasmonic acid (JA) and methyl-jasmonate (MeJA) were used for elicitation of Hypericum shoot cultures ( Sirvent and Gibson, 2002 ; Liu et al., 2007 ; Pavlík et al., 2007 ; Coste et al., 2011 ; Gadzovska et al., 2013 ).…”

Section: Phenotyping Of Secondary Metabolism In Response To Biotic/ab...mentioning

confidence: 99%

Does phenotyping of Hypericum secondary metabolism reveal a tolerance to biotic/abiotic stressors?

et al. 2022

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In this review we summarize the current knowledge about the changes in Hypericum secondary metabolism induced by biotic/abiotic stressors. It is known that the extreme environmental conditions activate signaling pathways leading to triggering of enzymatic and non-enzymatic defense systems, which stimulate production of secondary metabolites with antioxidant and protective effects. Due to several groups of bioactive compounds including naphthodianthrones, acylphloroglucinols, flavonoids, and phenylpropanes, the world-wide Hypericum perforatum represents a high-value medicinal crop of Hypericum genus, which belongs to the most diverse genera within flowering plants. The summary of the up-to-date knowledge reveals a relationship between the level of defense-related phenolic compounds and interspecific differences in the stress tolerance. The chlorogenic acid, and flavonoids, namely the amentoflavone, quercetin or kaempferol glycosides have been reported as the most defense-related metabolites associated with plant tolerance against stressful environment including temperature, light, and drought, in association with the biotic stimuli resulting from plant-microbe interactions. As an example, the species-specific cold-induced phenolics profiles of 10 Hypericum representatives of different provenances cultured in vitro are illustrated in the case-study. Principal component analysis revealed a relationship between the level of defense-related phenolic compounds and interspecific differences in the stress tolerance indicating a link between the provenance of Hypericum species and inherent mechanisms of cold tolerance. The underlying metabolome alterations along with the changes in the activities of ROS-scavenging enzymes, and non-enzymatic physiological markers are discussed. Given these data it can be anticipated that some Hypericum species native to divergent habitats, with interesting high-value secondary metabolite composition and predicted high tolerance to biotic/abiotic stresses would attract the attention as valuable sources of bioactive compounds for many medicinal purposes.

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Section: Phenotyping Of Secondary Metabolism In Response To Biotic/ab...mentioning

confidence: 99%

Does phenotyping of Hypericum secondary metabolism reveal a tolerance to biotic/abiotic stressors?

et al. 2022

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“…Increased aboveground vegetative biomass growth induced with Tria was also reported for spinach plants, the same degree of stimulation being achieved with 25 nM and 1 µM concentrations (Tompa et al, 2022). For BA, the highest shoot number was obtained when 2 mg L -1 BA was added to the nutrient medium of in vitro shoot cultures of St. John's wort, while the highest hypericin percentage in the fresh biomass was achieved when 1 mg L -1 BA was supplied exogenously (Karakas et al, 2009). It is worth mentioning that the plantlets produce their own quantity of cytokinins, and the added benzyladenine is an extra amount which increases the overall cytokinin content, also modifying the molar ratio between the different growth regulators, thus leading to further changes in growth and development (Li et al, 2020).…”

Section: Influences On Vegetative Growth and Developmentmentioning

confidence: 99%

Stimulation of Physiological Processes in St. John’s Wort (Hypericum perforatum L.) Seedlings by Treatments with Triacontanol and Benzyladenine

Fodorpataki

Berkeczi

Lunka

2021

Acta Biologica Marisiensis

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Treatment of St. John’s wort plantlets with 1 µM triacontanol and 2 µM benzyladenine stimulates growth and metabolic processes, being an environmental-friendly approach for optimizing the cultivation of these valuable medicinal plants under controlled conditions. When the two growth regulators (a bioactive cuticular wax constituent and a cytokinin) are applied simultaneously, they act synergistically, enhancing each other’s effect on the biomass accumulation and on certain parameters of the photosynthetic light use efficiency, such as the effective quantum yield of photosystem II and the overall vitality index of the photosynthetic apparatus which performs the conversion of light energy into usable forms for carbon dioxide assimilation. The results concerning the interactions between the two externally applied growth regulators during the early development of St. John’s wort plants may lead to a more efficient cultivation of this herbal medicinal product, including the possibility to modulate the production of pharmacologically active metabolites.

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“…In addition to the clonal multiplication procedure designed for H. perforatum ( Čellárová et al, 1992 ), the in vitro systems involving both, other wide-spread cosmopolitan, and endemic Hypericum species have been established for H. erectum ( Yazaki and Okuda, 1990 ) , H. canariense ( Mederos Molina, 1991 ), H. brasiliense ( Cardoso and de Oliveira, 1996 ) , H. balearicum, H. glandulosum, H. tomentosum, H. maculatum, H. olympicum , and H. bithynicum ( Kartnig et al, 1996 ), H. foliosum ( Moura, 1998 ), H. patulum ( Baruah et al, 2001 ) , H. androsaemum ( Guedes et al, 2003 ), H. heterophyllum ( Ayan and Cirak, 2006 ), H. polyanthemum ( Bernardi et al, 2007 ), H. hookerianum ( Padmesh et al, 2008 ), H. mysorense , ( Shilpashree and Ravishankar Rai, 2009 ), H. frondosum, H. kalmianum , and H. galioides ( Meyer et al, 2009 ), H. triquetrifolium ( Karakas et al, 2009 ; Oluk and Orhan, 2009 ), H. retusum ( Namli et al, 2010 ) , H. rumeliacum, H. tetrapterum, H. calycinum ( Danova, 2010 ) , H. richeri ssp. transsilvanicum, H. umbellatum A. Kern.…”

Section: Introductionmentioning

confidence: 99%

Conservation Strategies in the Genus Hypericum via Cryogenic Treatment

Bruňáková

Čellárová

2016

Front. Plant Sci.

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In the genus Hypericum, cryoconservation offers a strategy for maintenance of remarkable biodiversity, emerging from large inter- and intra-specific variability in morphological and phytochemical characteristics. Long-term cryostorage thus represents a proper tool for preservation of genetic resources of endangered and threatened Hypericum species or new somaclonal variants with unique properties. Many representatives of the genus are known as producers of pharmacologically important polyketides, namely naphthodianthrones and phloroglucinols. As a part of numerous in vitro collections, the nearly cosmopolitan Hypericum perforatum – Saint John’s wort – has become a suitable model system for application of biotechnological approaches providing an attractive alternative to the traditional methods for secondary metabolite production. The necessary requirements for efficient cryopreservation include a high survival rate along with an unchanged biochemical profile of plants regenerated from cryopreserved cells. Understanding of the processes which are critical for recovery of H. perforatum cells after the cryogenic treatment enables establishment of cryopreservation protocols applicable to a broad number of Hypericum species. Among them, several endemic taxa attract a particular attention due to their unique characteristics or yet unrevealed spectrum of bioactive compounds. In this review, recent advances in the conventional two-step and vitrification-based cryopreservation techniques are presented in relation to the recovery rate and biosynthetic capacity of Hypericum spp. The pre-cryogenic treatments which were identified to be crucial for successful post-cryogenic recovery are discussed. Being a part of genetic predisposition, the freezing tolerance as a necessary precondition for successful post-cryogenic recovery is pointed out. Additionally, a beneficial influence of cold stress on modulating naphthodianthrone biosynthesis is outlined.

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Effects of different concentrations of benzylaminopurine on shoot regeneration and hypericin content inHypericum triquetrifoliumTurra.

Cited by 13 publications

References 13 publications

Does phenotyping of Hypericum secondary metabolism reveal a tolerance to biotic/abiotic stressors?

Does phenotyping of Hypericum secondary metabolism reveal a tolerance to biotic/abiotic stressors?

Stimulation of Physiological Processes in St. John’s Wort (Hypericum perforatum L.) Seedlings by Treatments with Triacontanol and Benzyladenine

Conservation Strategies in the Genus Hypericum via Cryogenic Treatment

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